Genome-Wide Characterization of the Maize (Zea mays L.) WRKY Transcription Factor Family and Their Responses to Ustilago maydis
Abstract
:1. Introduction
2. Results
2.1. WRKY Genes Play Important Regulatory Roles in Response to U. maydis Inoculation
2.2. ZmWRKY Gene Identification and Characterization
2.3. Phylogenetic and Conserved Motif Analyses of ZmWRKY Family Genes
2.4. Synteny Analyses of the ZmWRKY Gene Family
2.5. Analyses of Tissue-Specific ZmWRKY Gene Expression Patterns
2.6. Analyses of U. maydis Infection-Related Changes in ZmWRKY Gene Expression Patterns
2.7. Identification of ZmWRKY Proteins Capable of Binding the ZmSWEET4b Promoter
2.8. Subcellular Localization Analyses
3. Discussion
4. Materials and Methods
4.1. Plant Materials and U. maydis Inoculation
4.2. Genome-Wide Identification of the ZmWRKY TF Family
4.3. Phylogenetic Classification, Chromosomal Localization, Gene Structure, and Motif Analyses
4.4. Duplication and Synteny Analyses of ZmWRKY Gene Family
4.5. RNA-Seq and Weighted Gene Co-Expression Network Analyses
4.6. qPCR Validation
4.7. Yeast One-Hybrid Assay
4.8. Subcellular Localization Analyses
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Wang, Y.; Li, W.; Qu, J.; Li, F.; Du, W.; Weng, J. Genome-Wide Characterization of the Maize (Zea mays L.) WRKY Transcription Factor Family and Their Responses to Ustilago maydis. Int. J. Mol. Sci. 2023, 24, 14916. https://doi.org/10.3390/ijms241914916
Wang Y, Li W, Qu J, Li F, Du W, Weng J. Genome-Wide Characterization of the Maize (Zea mays L.) WRKY Transcription Factor Family and Their Responses to Ustilago maydis. International Journal of Molecular Sciences. 2023; 24(19):14916. https://doi.org/10.3390/ijms241914916
Chicago/Turabian StyleWang, Yang, Wangshu Li, Jianzhou Qu, Fenghai Li, Wanli Du, and Jianfeng Weng. 2023. "Genome-Wide Characterization of the Maize (Zea mays L.) WRKY Transcription Factor Family and Their Responses to Ustilago maydis" International Journal of Molecular Sciences 24, no. 19: 14916. https://doi.org/10.3390/ijms241914916