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Article

Identification of NCAPG as an Essential Gene for Neuroblastoma Employing CRISPR-Cas9 Screening Database and Experimental Verification

by
Yubin Jia
1,2,3,4,†,
Jiaxing Yang
1,2,3,4,†,
Yankun Chen
1,2,3,4,
Yun Liu
1,2,3,4,
Yan Jin
1,2,3,4,
Chaoyu Wang
1,2,3,4,
Baocheng Gong
1,2,3,4,* and
Qiang Zhao
1,2,3,4,*
1
Department of Pediatric Oncology, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, China
2
National Clinical Research Center for Cancer, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, China
3
Key Laboratory of Cancer Prevention and Therapy, Tianjin 300060, China
4
Tianjin’s Clinical Research Center for Cancer, Tianjin 300060, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Int. J. Mol. Sci. 2023, 24(19), 14946; https://doi.org/10.3390/ijms241914946
Submission received: 14 July 2023 / Revised: 7 September 2023 / Accepted: 2 October 2023 / Published: 6 October 2023
(This article belongs to the Special Issue Cancer Biomarkers and Bioinformatics)
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Abstract

Neuroblastoma is the most common extracranial solid tumor in children. Patients with neuroblastoma have a poor prognosis. The development of therapy targets and the ability to predict prognosis will be enhanced through further exploration of the genetically related genes of neuroblastoma. The present investigation utilized CRISPR-Cas9 genome-wide screening based on the DepMap database to determine essential genes for neuroblastoma cells’ continued survival. WGCNA analysis was used to determine the progression-related genes, and a prognostic signature was constructed. The signature gene, NCAPG, was downregulated in neuroblastoma cells to explore its impact on various cellular processes. This research used DepMap and WGCNA to pinpoint 45 progression-related essential genes for neuroblastoma. A risk signature comprising NCAPG and MAD2L1 was established. The suppression of NCAPG prevented neuroblastoma cells from proliferating, migrating, and invading. The results of flow cytometric analysis demonstrated that NCAPG inhibition caused cell cycle arrest during the G2 and S phases and the activation of apoptosis. Additionally, NCAPG downregulation activated the p53-mediated apoptotic pathway, inducing cell apoptosis. The present work showed that NCAPG knockdown reduced neuroblastoma cell progression and may serve as a basis for further investigation into diagnostic indicators and therapy targets for neuroblastoma.
Keywords: neuroblastoma; Depmap; CRISPR-Cas9; NCAPG; risk signature; proliferation neuroblastoma; Depmap; CRISPR-Cas9; NCAPG; risk signature; proliferation

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MDPI and ACS Style

Jia, Y.; Yang, J.; Chen, Y.; Liu, Y.; Jin, Y.; Wang, C.; Gong, B.; Zhao, Q. Identification of NCAPG as an Essential Gene for Neuroblastoma Employing CRISPR-Cas9 Screening Database and Experimental Verification. Int. J. Mol. Sci. 2023, 24, 14946. https://doi.org/10.3390/ijms241914946

AMA Style

Jia Y, Yang J, Chen Y, Liu Y, Jin Y, Wang C, Gong B, Zhao Q. Identification of NCAPG as an Essential Gene for Neuroblastoma Employing CRISPR-Cas9 Screening Database and Experimental Verification. International Journal of Molecular Sciences. 2023; 24(19):14946. https://doi.org/10.3390/ijms241914946

Chicago/Turabian Style

Jia, Yubin, Jiaxing Yang, Yankun Chen, Yun Liu, Yan Jin, Chaoyu Wang, Baocheng Gong, and Qiang Zhao. 2023. "Identification of NCAPG as an Essential Gene for Neuroblastoma Employing CRISPR-Cas9 Screening Database and Experimental Verification" International Journal of Molecular Sciences 24, no. 19: 14946. https://doi.org/10.3390/ijms241914946

APA Style

Jia, Y., Yang, J., Chen, Y., Liu, Y., Jin, Y., Wang, C., Gong, B., & Zhao, Q. (2023). Identification of NCAPG as an Essential Gene for Neuroblastoma Employing CRISPR-Cas9 Screening Database and Experimental Verification. International Journal of Molecular Sciences, 24(19), 14946. https://doi.org/10.3390/ijms241914946

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