Insight into the Global Negative Regulation of Iron Scavenger 7-HT Biosynthesis by the SigW/RsiW System in Pseudomonas donghuensis HYS
Abstract
:1. Introduction
2. Results
2.1. Response of ECF σ Factors to Extracellular Ferrous Iron in P. donghuensis HYS
2.2. SigW Is Only Regulated by RsiW under Iron-Limiting Conditions in P. donghuensis HYS
2.3. The SigW/RsiW System Is Involved in Iron Metabolism and 7-HT Biosynthesis
2.4. SigW Inhibits the Production of Two Siderophores: Pyoverdine and 7-HT
2.5. SigW Directly Regulates the Expression of the nfs Cluster
2.6. Transcription Start Sites for the SigW Operon
2.7. SigW Negatively Regulates the Gac/Rsm System to Inhibit 7-HT and Enhance Pyoverdine Production
3. Discussion
4. Materials and Methods
4.1. Bacterial Strains and Culture Conditions
4.2. Construction of Deletion Mutants
4.3. Siderophore Determination Assays
4.4. Biofilm Formation Analysis
4.5. RNA Isolation and RT-PCR
4.6. Protein Expression and Purification
4.7. Promoter Activity Assay
4.8. Electrophoretic Mobility Shift Assay
4.9. 5′RACE PCR Analysis to Identify Transcription Start Sites
4.10. Bioinformatic Analysis
4.11. Accession Numbers
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Teng, S.; Wu, T.; Gao, D.; Wu, S.; Xiao, Y.; Long, Y.; Xie, Z. Insight into the Global Negative Regulation of Iron Scavenger 7-HT Biosynthesis by the SigW/RsiW System in Pseudomonas donghuensis HYS. Int. J. Mol. Sci. 2023, 24, 1184. https://doi.org/10.3390/ijms24021184
Teng S, Wu T, Gao D, Wu S, Xiao Y, Long Y, Xie Z. Insight into the Global Negative Regulation of Iron Scavenger 7-HT Biosynthesis by the SigW/RsiW System in Pseudomonas donghuensis HYS. International Journal of Molecular Sciences. 2023; 24(2):1184. https://doi.org/10.3390/ijms24021184
Chicago/Turabian StyleTeng, Shiyu, Tingting Wu, Donghao Gao, Siyi Wu, Yaqian Xiao, Yan Long, and Zhixiong Xie. 2023. "Insight into the Global Negative Regulation of Iron Scavenger 7-HT Biosynthesis by the SigW/RsiW System in Pseudomonas donghuensis HYS" International Journal of Molecular Sciences 24, no. 2: 1184. https://doi.org/10.3390/ijms24021184