Advanced Glycation End Products and Activation of Toll-like Receptor-2 and -4 Induced Changes in Aquaporin-3 Expression in Mouse Keratinocytes
Abstract
:1. Introduction
2. Results
2.1. AGE-BSA Used Initially Was Contaminated with Endotoxin
2.2. TLR2 Activation Downregulated AQP3 mRNA Expression; TLR2/TLR4 Activation Upregulated AQP3 Protein in the Absence of SAHA, and TLR2 Activation Downregulated AQP3 Protein in the Presence of SAHA in Primary Mouse Epidermal Keratinocytes
2.3. AGE-BSA from BioVision, Inc. Was Not Contaminated with TLR-Activating Endotoxin
2.4. AGEs Increased AQP3 Protein in the Absence of SAHA but Reduced AQP3 Protein in Its Presence in Primary Mouse Epidermal Keratinocytes
2.5. In Primary Mouse Epidermal Keratinocytes, AGEs, but Not SAHA, Increased Cell Number, a Measure of Proliferation
3. Discussion
4. Materials and Methods
4.1. Cell Culture and Treatment
4.2. Quantitative RT-PCR
4.3. Western Blotting
4.4. BSA-AGEs Contamination Test
4.5. MTT Assay
4.6. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Luo, Y.; Uaratanawong, R.; Choudhary, V.; Hardin, M.; Zhang, C.; Melnyk, S.; Chen, X.; Bollag, W.B. Advanced Glycation End Products and Activation of Toll-like Receptor-2 and -4 Induced Changes in Aquaporin-3 Expression in Mouse Keratinocytes. Int. J. Mol. Sci. 2023, 24, 1376. https://doi.org/10.3390/ijms24021376
Luo Y, Uaratanawong R, Choudhary V, Hardin M, Zhang C, Melnyk S, Chen X, Bollag WB. Advanced Glycation End Products and Activation of Toll-like Receptor-2 and -4 Induced Changes in Aquaporin-3 Expression in Mouse Keratinocytes. International Journal of Molecular Sciences. 2023; 24(2):1376. https://doi.org/10.3390/ijms24021376
Chicago/Turabian StyleLuo, Yonghong, Rawipan Uaratanawong, Vivek Choudhary, Mary Hardin, Catherine Zhang, Samuel Melnyk, Xunsheng Chen, and Wendy B. Bollag. 2023. "Advanced Glycation End Products and Activation of Toll-like Receptor-2 and -4 Induced Changes in Aquaporin-3 Expression in Mouse Keratinocytes" International Journal of Molecular Sciences 24, no. 2: 1376. https://doi.org/10.3390/ijms24021376