An Analytical Method for Quantifying the Yields of DNA Double-Strand Breaks Coupled with Strand Breaks by γ-H2AX Focus Formation Assay Based on Track-Structure Simulation
Abstract
:1. Introduction
2. Results and Discussion
2.1. Spatial Distribution of γ-H2AX Foci and Foci Area
2.2. Relationship between γ-H2AX Foci Area and Additional Strand Break Induction
3. Materials and Methods
3.1. Cell Line and Cell Culture
3.2. Irradiation Setup
3.3. Detection of DSBs by γ-H2AX Focus Formation Assay
3.4. Calculation of Theoretical Data for the Distance between Two γ-H2AX Foci
3.5. Simulation Setup
3.6. Calculation of DNA Damage Complexity for X-ray Irradiation
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Yachi, Y.; Matsuya, Y.; Yoshii, Y.; Fukunaga, H.; Date, H.; Kai, T. An Analytical Method for Quantifying the Yields of DNA Double-Strand Breaks Coupled with Strand Breaks by γ-H2AX Focus Formation Assay Based on Track-Structure Simulation. Int. J. Mol. Sci. 2023, 24, 1386. https://doi.org/10.3390/ijms24021386
Yachi Y, Matsuya Y, Yoshii Y, Fukunaga H, Date H, Kai T. An Analytical Method for Quantifying the Yields of DNA Double-Strand Breaks Coupled with Strand Breaks by γ-H2AX Focus Formation Assay Based on Track-Structure Simulation. International Journal of Molecular Sciences. 2023; 24(2):1386. https://doi.org/10.3390/ijms24021386
Chicago/Turabian StyleYachi, Yoshie, Yusuke Matsuya, Yuji Yoshii, Hisanori Fukunaga, Hiroyuki Date, and Takeshi Kai. 2023. "An Analytical Method for Quantifying the Yields of DNA Double-Strand Breaks Coupled with Strand Breaks by γ-H2AX Focus Formation Assay Based on Track-Structure Simulation" International Journal of Molecular Sciences 24, no. 2: 1386. https://doi.org/10.3390/ijms24021386