Sex Differences in Brain Region-Specific Activation of c-Fos following Kappa Opioid Receptor Stimulation or Acute Stress in Mice
Abstract
:1. Introduction
2. Results
2.1. Effects of Systemic Administration of U50,488 on c-Fos Expression in the PFCx of Male and Female Mice
2.2. Effects of Acute FSS on c-Fos Expression in the PFCx of Male and Female Mice
2.3. Effects of Activating KOPr and Acute FSS on c-Fos Expression in the NAcc
2.4. Effects of Activating KOPr and Acute FSS on c-Fos Expression in the Hippocampus
2.5. Effects of Activating KOPr and Acute FSS on c-Fos Expression in the Amygdala
2.6. KOPr- and Acute FSS-Activated Cells Are Predominantly Non-GABAergic Neurons
3. Discussion
3.1. Sex Differences following Systemic KOPr Agonist Administration
3.2. Sex Differences following an Acute In Vivo Stressor
3.3. Functional Role of Sex-Dependent c-Fos Expression following KOPr Agonist Administration and In Vivo Stress
3.4. Conclusions
4. Materials and Methods
4.1. Animals
4.2. Drugs
4.3. Acute Forced Swim Stress (FSS)
4.4. Immunohistochemistry
4.5. Quantification and Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Male | U50,488 | Forced Swim Stress | ||||||
---|---|---|---|---|---|---|---|---|
c-Fos | c-Fos-GFP | c-Fos | c-Fos-GFP | |||||
Saline | norBNI | Saline | norBNI | Saline | norBNI | Saline | norBNI | |
PFCx | ↑↑ | ** | ↑ | * | ↑↑ | ns | ↑↑ | ns |
NAc | ↑↑ | ** | ↑ | ** | ↑↑ | * | ↑↑ | ns |
CA1 | ↑ | * | ↑↑ | ** | ns | ns | ns | ns |
DG | ns | ns | ns | Ns | ns | ns | ns | ns |
CeA | ↑ | * | ↑ | * | ns | ns | ns | ns |
BLA | ↑ | * | ↑↑ | ** | ↑ | ns | ↑ | ns |
Female | U50,488 | Forced swim stress | ||||||
c-Fos | c-Fos-GFP | c-Fos | c-Fos-GFP | |||||
Saline | norBNI | Saline | norBNI | Saline | norBNI | Saline | norBNI | |
PFCx | ns | ns | ns | ns | ns | ns | ns | ns |
NAc | ns | ns | ns | ns | ↑↑ | ns | ↑ | ns |
CA1 | ↑↑↑ | ** | ns | ns | ns | ns | ns | ns |
DG | ns | ns | ns | ns | ns | ns | ns | ns |
CeA | ↑ | * | ↑↑ | * | ns | ns | ns | ns |
BLA | ↑↑↑ | ** | ↑↑ | * | ↑↑↑ | ns | ↑ | ns |
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Ma, Q.; Wonnacott, S.; Bailey, S.J.; Bailey, C.P. Sex Differences in Brain Region-Specific Activation of c-Fos following Kappa Opioid Receptor Stimulation or Acute Stress in Mice. Int. J. Mol. Sci. 2023, 24, 15098. https://doi.org/10.3390/ijms242015098
Ma Q, Wonnacott S, Bailey SJ, Bailey CP. Sex Differences in Brain Region-Specific Activation of c-Fos following Kappa Opioid Receptor Stimulation or Acute Stress in Mice. International Journal of Molecular Sciences. 2023; 24(20):15098. https://doi.org/10.3390/ijms242015098
Chicago/Turabian StyleMa, Qianhan, Susan Wonnacott, Sarah J. Bailey, and Christopher P. Bailey. 2023. "Sex Differences in Brain Region-Specific Activation of c-Fos following Kappa Opioid Receptor Stimulation or Acute Stress in Mice" International Journal of Molecular Sciences 24, no. 20: 15098. https://doi.org/10.3390/ijms242015098