BMP-9 Improves the Osteogenic Differentiation Ability over BMP-2 through p53 Signaling In Vitro in Human Periosteum-Derived Cells
Abstract
:1. Introduction
2. Results
2.1. Osteogenic Potential of hPDCs Induced by BMP-9 In Vitro
2.2. Comparison of Intracellular Signaling Pathway Differences in BMP-Induced hPDCs
2.3. Reduction in Osteogenic Differentiation by Pifithrin-α
2.4. Mechanism of Action of BMP-9 and p53
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Culture and Osteogenic Differentiation of the hPDCs
4.3. Evaluation of Osteogenic Phenotype
4.4. Phosphorylation Array
4.5. Western Blot Analysis
4.6. Reverse Transcription and Quantitative RT-PCR (qRT-PCR) Using TaqMan Assays
4.7. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Park, J.-H.; Koh, E.-B.; Seo, Y.-J.; Oh, H.-S.; Byun, J.-H. BMP-9 Improves the Osteogenic Differentiation Ability over BMP-2 through p53 Signaling In Vitro in Human Periosteum-Derived Cells. Int. J. Mol. Sci. 2023, 24, 15252. https://doi.org/10.3390/ijms242015252
Park J-H, Koh E-B, Seo Y-J, Oh H-S, Byun J-H. BMP-9 Improves the Osteogenic Differentiation Ability over BMP-2 through p53 Signaling In Vitro in Human Periosteum-Derived Cells. International Journal of Molecular Sciences. 2023; 24(20):15252. https://doi.org/10.3390/ijms242015252
Chicago/Turabian StylePark, Jin-Ho, Eun-Byeol Koh, Young-Jin Seo, Hye-Seong Oh, and June-Ho Byun. 2023. "BMP-9 Improves the Osteogenic Differentiation Ability over BMP-2 through p53 Signaling In Vitro in Human Periosteum-Derived Cells" International Journal of Molecular Sciences 24, no. 20: 15252. https://doi.org/10.3390/ijms242015252