Comparative Genomics Identifies the Evolutionarily Conserved Gene TPM3 as a Target of eca-miR-1 Involved in the Skeletal Muscle Development of Donkeys
Abstract
:1. Introduction
2. Results
2.1. Comparative Genomics of Equine Genomes and Outgroup (Goat and Cattle)
2.2. The Karyotype Evolution of Equine Genomes
2.3. TPM3 Evolutionarily Conserved in Equus and Differentially Expressed in S1 and S2 Muscle
2.4. Eca-miR-1 Targeting the TPM3 Gene
2.5. The TPM3 Gene Family Evolution and Expression Profile
3. Discussion
3.1. Comparative Genomics and Collinearity Analysis Identify Muscle Development Gene TPM3 in Equus
3.2. Comparative Transcriptomics Reveals the TPM3 Gene Potentially Involved in Muscle Development in Donkeys
3.3. TPM3 Regulates Muscle Development Targeted by eca-miR-1
4. Materials and Methods
4.1. Ethics Statement
4.2. Comparative Genomics Analysis
4.3. Chromosome Collinearity Analysis Rearrangement Analysis
4.4. Sample Collection
4.5. RNA Extraction and Sequencing Data Processing
4.6. Identification of the miRNAs
4.7. Prediction and Validation of the miRNA Target Genes
4.8. Vector Construction, Cell Transfection, and Dual-Luciferase Reporter Assay
4.9. Gene Family Identification Analysis
4.10. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Yang, G.; Sun, M.; Wang, Z.; Hu, Q.; Guo, J.; Yu, J.; Lei, C.; Dang, R. Comparative Genomics Identifies the Evolutionarily Conserved Gene TPM3 as a Target of eca-miR-1 Involved in the Skeletal Muscle Development of Donkeys. Int. J. Mol. Sci. 2023, 24, 15440. https://doi.org/10.3390/ijms242015440
Yang G, Sun M, Wang Z, Hu Q, Guo J, Yu J, Lei C, Dang R. Comparative Genomics Identifies the Evolutionarily Conserved Gene TPM3 as a Target of eca-miR-1 Involved in the Skeletal Muscle Development of Donkeys. International Journal of Molecular Sciences. 2023; 24(20):15440. https://doi.org/10.3390/ijms242015440
Chicago/Turabian StyleYang, Ge, Minhao Sun, Zhaofei Wang, Qiaoyan Hu, Jiajun Guo, Jie Yu, Chuzhao Lei, and Ruihua Dang. 2023. "Comparative Genomics Identifies the Evolutionarily Conserved Gene TPM3 as a Target of eca-miR-1 Involved in the Skeletal Muscle Development of Donkeys" International Journal of Molecular Sciences 24, no. 20: 15440. https://doi.org/10.3390/ijms242015440