Radiocarbon Flux Measurements Reveal Mechanistic Insight into Heat-Stress Induction of Nicotine Biosynthesis in Nicotiana attenuata
Abstract
:1. Introduction
2. Results
2.1. Acute and Chronic Heat Stress Alters Tissue Nicotine Content
2.2. Effect of Heat Stress on 11CO2 Fixation and 13NO3− Uptake
2.3. Effect of Heat Stress on ‘New’ Carbon Partitioning (as 11C) into Amino Acids
2.4. Effect of Heat Stress on the Endogenous Concentrations of [12C]-Aspartic Acid and [12C]-Asparagine
2.5. Effect of Heat Stress on Leaf Total Protein and Rubisco Protein Content
3. Discussion
4. Materials and Methods
4.1. Plant Growth
4.2. Nicotine Analysis
4.3. Production and Administration of Radioactive 11CO2 and 13NO3−
4.4. [11/12C]-Aspartic Acid and [11/12C]-Asparagine Analyses
4.5. Protein Analysis
4.6. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Waller, S.; Powell, A.; Noel, R.; Schueller, M.J.; Ferrieri, R.A. Radiocarbon Flux Measurements Reveal Mechanistic Insight into Heat-Stress Induction of Nicotine Biosynthesis in Nicotiana attenuata. Int. J. Mol. Sci. 2023, 24, 15509. https://doi.org/10.3390/ijms242115509
Waller S, Powell A, Noel R, Schueller MJ, Ferrieri RA. Radiocarbon Flux Measurements Reveal Mechanistic Insight into Heat-Stress Induction of Nicotine Biosynthesis in Nicotiana attenuata. International Journal of Molecular Sciences. 2023; 24(21):15509. https://doi.org/10.3390/ijms242115509
Chicago/Turabian StyleWaller, Spenser, Avery Powell, Randi Noel, Michael J. Schueller, and Richard A. Ferrieri. 2023. "Radiocarbon Flux Measurements Reveal Mechanistic Insight into Heat-Stress Induction of Nicotine Biosynthesis in Nicotiana attenuata" International Journal of Molecular Sciences 24, no. 21: 15509. https://doi.org/10.3390/ijms242115509