A Strainer-Based Platform for the Collection and Immunolabeling of Porcine Epidemic Diarrhea Virus-Infected Porcine Intestinal Organoid
Abstract
:1. Introduction
2. Results
2.1. Summarization of Porcine Intestinal Organoids: Culturalization, Infection, Collection, and Fluorescence Imaging
2.2. Development of Intestinal Organoids from Porcine Inoculated
2.3. Collection, Labeling, and Imaging of Porcine Intestinal Organoids Using Cell Strainers with Different Aperture Sizes
2.4. Visualization of Porcine Intestinal Organoids Infected with PEDV
3. Discussion
4. Materials and Methods
4.1. Acquisition and Culture of Porcine Intestinal Organoids
4.2. Porcine Intestinal Organoids Infected with Porcine Epidemic Diarrhea Virus
4.3. Detection via Western Blotting
4.4. The Collection and Loading of Porcine Intestinal Organoids with the Strainer Platform
4.5. Labeling Process of PEDV-Infected or Uninfected Porcine Intestinal Organoids
4.6. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Liu, Y.; Tan, J.; Zhang, N.; Li, W.; Fu, B. A Strainer-Based Platform for the Collection and Immunolabeling of Porcine Epidemic Diarrhea Virus-Infected Porcine Intestinal Organoid. Int. J. Mol. Sci. 2023, 24, 15671. https://doi.org/10.3390/ijms242115671
Liu Y, Tan J, Zhang N, Li W, Fu B. A Strainer-Based Platform for the Collection and Immunolabeling of Porcine Epidemic Diarrhea Virus-Infected Porcine Intestinal Organoid. International Journal of Molecular Sciences. 2023; 24(21):15671. https://doi.org/10.3390/ijms242115671
Chicago/Turabian StyleLiu, Yinju, Jinlong Tan, Nianzhang Zhang, Wenhui Li, and Baoquan Fu. 2023. "A Strainer-Based Platform for the Collection and Immunolabeling of Porcine Epidemic Diarrhea Virus-Infected Porcine Intestinal Organoid" International Journal of Molecular Sciences 24, no. 21: 15671. https://doi.org/10.3390/ijms242115671