Immunobiotic Ligilactobacillus salivarius FFIG58 Confers Long-Term Protection against Streptococcus pneumoniae

Elean, Mariano; Raya Tonetti, Fernanda; Fukuyama, Kohtaro; Arellano-Arriagada, Luciano; Namai, Fu; Suda, Yoshihito; Gobbato, Nadia; Nishiyama, Keita; Villena, Julio; Kitazawa, Haruki

doi:10.3390/ijms242115773

Open AccessArticle

Immunobiotic Ligilactobacillus salivarius FFIG58 Confers Long-Term Protection against Streptococcus pneumoniae

by

Mariano Elean

^1,†,

Fernanda Raya Tonetti

^1,†

,

Kohtaro Fukuyama

^2,3,

Luciano Arellano-Arriagada

^1,2,

Fu Namai

^2,3,

Yoshihito Suda

⁴,

Nadia Gobbato

⁵,

Keita Nishiyama

^2,3,

Julio Villena

^1,2,*

and

Haruki Kitazawa

^2,3,*

¹

Laboratory of Immunobiotechnology, Reference Centre for Lactobacilli (CERELA-CONICET), Tucuman 4000, Argentina

²

Food and Feed Immunology Group, Laboratory of Animal Food Function, Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, Japan

³

Livestock Immunology Unit, International Education and Research Center for Food and Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, Japan

⁴

Department of Food, Agriculture and Environment, Miyagi University, Sendai 980-8572, Japan

⁵

Laboratory of Immunology, Microbiology Institute, Faculty of Biochemistry, Chemistry and Pharmacy, National University of Tucuman, Tucuman 4000, Argentina

^*

Authors to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

Int. J. Mol. Sci. 2023, 24(21), 15773; https://doi.org/10.3390/ijms242115773

Submission received: 2 October 2023 / Revised: 27 October 2023 / Accepted: 29 October 2023 / Published: 30 October 2023

(This article belongs to the Special Issue Molecular Research in Prebiotics, Probiotics and Postbiotics)

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Abstract

Previously, we isolated potentially probiotic Ligilactobacillus salivarius strains from the intestines of wakame-fed pigs. The strains were characterized based on their ability to modulate the innate immune responses triggered by the activation of Toll-like receptor (TLR)-3 or TLR4 signaling pathways in intestinal mucosa. In this work, we aimed to evaluate whether nasally administered L. salivarius strains are capable of modulating the innate immune response in the respiratory tract and conferring long-term protection against the respiratory pathogen Streptococcus pneumoniae. Infant mice (3-weeks-old) were nasally primed with L. salivarius strains and then stimulated with the TLR3 agonist poly(I:C). Five or thirty days after the last poly(I:C) administration mice were infected with pneumococci. Among the strains evaluated, L. salivarius FFIG58 had a remarkable ability to enhance the protection against the secondary pneumococcal infection by modulating the respiratory immune response. L. salivarius FFIG58 improved the ability of alveolar macrophages to produce interleukin (IL)-6, interferon (IFN)-γ, IFN-β, tumor necrosis factor (TNF)-α, IL-27, chemokine C-C motif ligand 2 (CCL2), chemokine C-X-C motif ligand 2 (CXCL2), and CXCL10 in response to pneumococcal challenge. Furthermore, results showed that the nasal priming of infant mice with the FFIG58 strain protected the animals against secondary infection until 30 days after stimulation with poly(I:C), raising the possibility of using nasally administered immunobiotics to stimulate trained immunity in the respiratory tract.

Keywords: Ligilactobacillus salivarius FFIG58; respiratory superinfection; Streptococcus pneumoniae; poly(I:C); antiviral immunity; immunobiotics

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MDPI and ACS Style

Elean, M.; Raya Tonetti, F.; Fukuyama, K.; Arellano-Arriagada, L.; Namai, F.; Suda, Y.; Gobbato, N.; Nishiyama, K.; Villena, J.; Kitazawa, H. Immunobiotic Ligilactobacillus salivarius FFIG58 Confers Long-Term Protection against Streptococcus pneumoniae. Int. J. Mol. Sci. 2023, 24, 15773. https://doi.org/10.3390/ijms242115773

AMA Style

Elean M, Raya Tonetti F, Fukuyama K, Arellano-Arriagada L, Namai F, Suda Y, Gobbato N, Nishiyama K, Villena J, Kitazawa H. Immunobiotic Ligilactobacillus salivarius FFIG58 Confers Long-Term Protection against Streptococcus pneumoniae. International Journal of Molecular Sciences. 2023; 24(21):15773. https://doi.org/10.3390/ijms242115773

Chicago/Turabian Style

Elean, Mariano, Fernanda Raya Tonetti, Kohtaro Fukuyama, Luciano Arellano-Arriagada, Fu Namai, Yoshihito Suda, Nadia Gobbato, Keita Nishiyama, Julio Villena, and Haruki Kitazawa. 2023. "Immunobiotic Ligilactobacillus salivarius FFIG58 Confers Long-Term Protection against Streptococcus pneumoniae" International Journal of Molecular Sciences 24, no. 21: 15773. https://doi.org/10.3390/ijms242115773

APA Style

Elean, M., Raya Tonetti, F., Fukuyama, K., Arellano-Arriagada, L., Namai, F., Suda, Y., Gobbato, N., Nishiyama, K., Villena, J., & Kitazawa, H. (2023). Immunobiotic Ligilactobacillus salivarius FFIG58 Confers Long-Term Protection against Streptococcus pneumoniae. International Journal of Molecular Sciences, 24(21), 15773. https://doi.org/10.3390/ijms242115773

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Immunobiotic Ligilactobacillus salivarius FFIG58 Confers Long-Term Protection against Streptococcus pneumoniae

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