DLGAP5 Regulates the Proliferation, Migration, Invasion, and Cell Cycle of Breast Cancer Cells via the JAK2/STAT3 Signaling Axis
Abstract
:1. Introduction
2. Results
2.1. Identification of DEGs in BC Based on Bioinformatic Methods
2.2. Screening BC-Related Genes through WGCNA
2.3. PPI Network Construction of Intersection Genes
2.4. Key Genes Screening in BC
2.5. Differential Expression, Survival Analysis, and Diagnostic Accuracy Analysis of Key Genes
2.6. Cell Localization and Expression Verification of DLGAP5
2.7. DLGAP5 Affected the Proliferation and Colony Formation of BC Cell Lines
2.8. DLGAP5 Inhibited the Migration and Invasion of BC Cells
2.9. DLGAP5 Affected the Cell Cycle of BC Cell
2.10. DLGAP5 Regulated JAK2/STAT3 Signaling Pathway in BC Cell Lines
3. Discussion
4. Materials and Methods
4.1. Data Sources and Differential Analysis
4.2. WGCNA Construction and Module Identification
4.3. Protein–Protein Interaction Networks (PPI) Construction
4.4. Key Gene Screening
4.5. Survival, Differential Expression and Diagnostic Accuracy Analyses of Key Genes
4.6. Analysis of the Single-Cell RNA Sequencing Dataset
4.7. Enrichment Analysis of DLGAP5
4.8. Cell Culture and Stable Cell Line Construction
4.9. RNA Extraction and qRT-PCR
4.10. Western Blot
4.11. CCK8
4.12. Colony Formation Assay
4.13. Wound-Healing Assay
4.14. Transwell Assay
4.15. Flow Cytometry
4.16. Data Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Li, Y.; Wei, J.; Sun, Y.; Zhou, W.; Ma, X.; Guo, J.; Zhang, H.; Jin, T. DLGAP5 Regulates the Proliferation, Migration, Invasion, and Cell Cycle of Breast Cancer Cells via the JAK2/STAT3 Signaling Axis. Int. J. Mol. Sci. 2023, 24, 15819. https://doi.org/10.3390/ijms242115819
Li Y, Wei J, Sun Y, Zhou W, Ma X, Guo J, Zhang H, Jin T. DLGAP5 Regulates the Proliferation, Migration, Invasion, and Cell Cycle of Breast Cancer Cells via the JAK2/STAT3 Signaling Axis. International Journal of Molecular Sciences. 2023; 24(21):15819. https://doi.org/10.3390/ijms242115819
Chicago/Turabian StyleLi, Yujie, Jie Wei, Yao Sun, Wenqian Zhou, Xiaoya Ma, Jinping Guo, Huan Zhang, and Tianbo Jin. 2023. "DLGAP5 Regulates the Proliferation, Migration, Invasion, and Cell Cycle of Breast Cancer Cells via the JAK2/STAT3 Signaling Axis" International Journal of Molecular Sciences 24, no. 21: 15819. https://doi.org/10.3390/ijms242115819