Untargeted Lipidomics Study of Bipolar Disorder Patients in Serbia
Abstract
:1. Introduction
2. Results
Selection of Potential Lipid Biomarkers
3. Discussion
4. Materials and Methods
4.1. Sample and Sample Preparation
4.2. Chemicals
4.3. Lipidomics Analysis
4.3.1. Lipid Extraction from Blood Serum Samples
4.3.2. Liquid Chromatography–High-Resolution Mass Spectrometry (LC–HRMS) Measurements
4.4. LC–HRMS Data Processing and Statistical Analysis
4.5. Identification of Potential Metabolite Biomarkers
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Variable | BD (N = 31) | C (N = 31) |
---|---|---|
Nationality; race (%) | Serbian; white (100%) | Serbian; white (100%) |
Medications use, N (%) | ||
Antipsychotics of the first generation | 2 (6.45%) | / |
Antipsychotics of the second generation | 24 (77.42%) | / |
Anxiolytics | 5 (16.13%) | / |
Smokers, N(%) | 22 (70.97%) | 18 (58.06%) |
Age range (mean) | 20–74 (48.12) | 24–54 (47.86) |
No. | Retention Time (min) | VIP Value | VIP Feature Assignment | Measured m/z * | Ion Mode Adduct | Proposed Formula | Lipid Assignment | Lipid Class |
---|---|---|---|---|---|---|---|---|
1 | 1.29 | 1.5673 | LPC 18:2 A | 578.3494 | [M+OAc]− | C26H50NO7P | LPC 18:2 | GP |
1.4548 | LPC 18:2 A1 | 520.3385 | [M+H]+ | |||||
1.3381 | LPC 18:2 A2 | 542.3228 | [M+Na]+ | |||||
2 | 1.52 | 1.4205 | LPC 16:0 A2 | 496.3385 | [M+H]+ | C24H50NO7P | LPC 16:0 | GP |
3 | 2.15 | 1.3184 | FA 24:0;O | 402.3924 | [M+NH4]+ | C24H48O3 | FA 24:0;O | FA |
4 | 2.15 | 1.2832 | FA 22:0 | 358.3661 | [M+NH4]+ | C22H44O2 | FA 22:0 | FA |
5 | 3.12 | 1.0837 | PA 25:0 | 589.3263 | [M+K]+ | C28H55O8P | PA 25:0 | GP |
6 | 3.12 | 1.1387 | LPS O-24:1;O | 632.3937 | [M+Na]+ | C30H60NO9P | LPS O-24:1;O | GP |
7 | 3.35 | 1.1909 | FA 21:4;O6 | 415.2349 | [M+H]+ | C21H34O8 | FA 21:4;O6 | FA |
8 | 4.77 | 1.1020 | Cer 34:1;O2 A | 560.5026 | [M+Na]+ | C34H67NO3 | Cer 34:1;O2 | SP |
9 | 5.09 | 1.3798 | SM 32:1;O2 | 675.5427 | [M+H]+ | C37H75N2O6P | SM 32:1;O2 | SP |
10 | 5.17 | 1.3764 | SM 34:2;O2 A1 | 701.5587 | [M+H]+ | C39H77N2O6P | SM 34:2;O2 | SP |
11 | 5.43 | 1.2202 | SM 33:1;O2 | 689.5584 | [M+H]+ | C38H77N2O6P | SM 33:1;O2 | SP |
12 | 5.63 | 1.6594 | PC 38:6 A | 806.5691 | [M+H]+ | C46H80NO8P | PC 38:6 | SP |
13 | 5.73 | 1.9145 | PC 36:4 | 782.5695 | [M+H]+ | C44H80NO8P | PC 36:4 | GP |
14 | 5.75 | 1.2116 | SM 34:1;O2 A2 | 725.5564 | [M+Na]+ | C39H79N2O6P | SM 34:1;O2 | SP |
1.3527 | SM 34:1;O2 A1 | 703.5748 | [M+H]+ | |||||
15 | 5.95 | 1.3059 | SM 34:1;O2 | 703.5746 | [M+H]+ | C39H79N2O6P | SM 34:1;O2 | SP |
16 | 6.04 | 1.4245 | PC 36:4 B5 | 782.5716 | [M+H]+ | C44H80NO8P | PC 36:4 | GP |
17 | 6.09 | 1.0887 | PC 33:2 B | 802.5619 | [M+OAc]− | C41H78NO8P | PC 33:2 | GP |
18 | 6.12 | 1.7568 | PC 34:2 A | 816.5779 | [M+OAc]− | C42H80NO8P | PC 34:2 | GP |
1.6487 | PC 34:2 A2 | 1516.1288 | [2M+H]+ | |||||
1.6482 | PC 34:2 A3 | 796.5254 | [M+K]+ | |||||
1.6229 | PC 34:2 A4 | 780.5515 | [M+Na]+ | |||||
1.5566 | PC 34:2 A5 | 758,5787 | [M+H]+ | |||||
1.1697 | PC 34:2 A6 | 184.0725 | [C5H14NO4P+H]+ | |||||
19 | 6.37 | 1.5682 | PC O-36:5 | 766.5744 | [M+H]+ | C44H80NO7P | PC O-36:5 | GP |
20 | 6.38 | 1.0028 | PC O-36:4 A1 | 826.5976 | [M+OAc]− | C44H82NO7P | PC O-36:4 | GP |
21 | 6.46 | 1.4419 | PC O-38:6 | 792.5894 | [M+H]+ | C46H82NO7P | PC O-38:6 | GP |
22 | 6.50 | 1.8977 | PC O-34:3 | 742.5741 | [M+H]+ | C42H80NO7P | PC O-34:3 | GP |
23 | 6.51 | 1.1837 | PC 35:2 A1 | 772.5849 | [M+H]+ | C43H82NO8P | PC 35:2 | GP |
24 | 6.53 | 1.9588 | PC O-36:4 | 768.5900 | [M+H]+ | C44H82NO7P | PC O-36:4 | GP |
25 | 6.60 | 1.6051 | PC O-38:5 A | 794.6057 | [M+H]+ | C46H84NO7P | PC O-38:5 | GP |
26 | 6.64 | 2.1701 | PC O-34:2 | 744.5895 | [M+H]+ | C42H82NO7P | PC O-34:2 | GP |
27 | 6.73 | 1.9050 | PC O-36:3 | 770.6046 | [M+H]+ | C44H84NO7P | PC O-36:3 | GP |
28 | 6.83 | 1.2700 | PC 35:2 | 830.5927 | [M+H]+ | C43H82NO8P | PC 35:2 | GP |
29 | 6.83 | 1.8175 | PC 36:2 A | 844.6088 | [M+OAc]− | C44H84NO8P | PC 36:2 | GP |
1.6528 | PC 36:2 A2 | 808.5828 | [M+Na]+ | |||||
1.6180 | PC 36:2 A3 | 824.5563 | [M+K]+ | |||||
30 | 6.87 | 1.6510 | PS 41:4 | 876.5696 | [M+Na]+ | C47H84NO10P | PS 41:4 | GP |
31 | 6.95 | 1.4782 | PC O-32:1 | 718.5736 | [M+H]+ | C40H80NO7P | PC O-32:1 | GP |
32 | 6.97 | 1.2903 | Cer 36:0;O2 | 568.5651 | [M+H]+ | C36H73NO3 | Cer 36:0;O2 | SP |
33 | 7.08 | 1.9935 | PC O-40:6 | 837.6194 | [M+NH4]+ | C48H86NO7P | PC O-40:6 | GP |
34 | 7.08 | 1.2704 | PC O-34:2 | 744.5891 | [M+H]+ | C42H82NO7P | PC O-34:2 | GP |
35 | 7.11 | 1.5948 | PC O-32:0 | 720.5892 | [M+H]+ | C40H82NO7P | PC O-32:0 | GP |
36 | 7.14 | 1.4019 | PC O-38:5 B | 794.6051 | [M+H]+ | C46H84NO7P | PC O-38:5 | GP |
37 | 7.22 | 1.4158 | PC O-34:1 | 746.6050 | [M+H]+ | C42H84NO7P | PC O-34:1 | GP |
38 | 7.31 | 1.6438 | PC O-38:4 | 796.6209 | [M+H]+ | C46H86NO7P | PC O-38:4 | GP |
39 | 7.34 | 1.4761 | SM 38:1;O2 | 759.6370 | [M+H]+ | C43H87N2O6P | SM 38:1;O2 | SP |
40 | 7.36 | 1.3589 | PC 34:0 | 762.5997 | [M+H]+ | C42H84NO8P | PC 34:0 | GP |
41 | 7.46 | 1.6474 | SM 40:2;O2 | 785.6529 | [M+H]+ | C45H89N2O6P | SM 40:2;O2 | SP |
42 | 7.88 | 1.1853 | SM 41:2;O2 | 799.6680 | [M+H]+ | C46H91N2O6P | SM 41:2;O2 | SP |
43 | 8.09 | 1.9568 | SM 40:1;O2 | 787.6687 | [M+H]+ | C45H91N2O6P | SM 40:1;O2 | SP |
44 | 8.49 | 1.7303 | SM 41:1;O2 | 801.6839 | [M+H]+ | C46H93N2O6P | SM 41:1;O2 | SP |
45 | 8.86 | 2.0248 | SM 42:1;O2 | 815.6997 | [M+H]+ | C47H95N2O6P | SM 42:1;O2 | SP |
46 | 9.75 | 1.5408 | DG 37:7 | 647.4579 | [M+Na]+ | C40H64O5 | DG 37:7 | GL |
47 | 9.96 | 1.7561 | Cer 42:1;O2 | 672.6257 | [M+Na]+ | C42H83NO3 | Cer 42:1;O2 | SP |
48 | 11.31 | 1.0632 | TG 52:4 A1 | 872.7703 | [M+NH4]+ | C55H98O6 | TG 52:4 | GL |
1.0751 | TG 52:4 A2 | 877.7257 | [M+Na]+ | |||||
1.0570 | TG 52:4 A3 | 893.6996 | [M+K]+ | |||||
49 | 11.31 | 1.1380 | TG 58:4 | 956.8632 | [M+NH4]+ | C61H110O6 | TG 58:4 | GL |
50 | 11.49 | 1.1909 | TG 52:3 A1 | 874.7863 | [M+NH4]+ | C55H100O6 | TG 52:3 | GL |
1.2129 | TG 52:3 A2 | 879.7414 | [M+Na]+ | |||||
1.0899 | TG 52:3 A3 | 895.7152 | [M+K]+ | |||||
51 | 11.49 | 1.2905 | TG 58:3 | 958.8792 | [M+NH4]+ | C61H112O6 | TG 58:3 | GL |
52 | 11.55 | 1.1582 | TG 49:1 | 836.7694 | [M+NH4]+ | C52H98O6 | TG 49:1 | GL |
53 | 11.58 | 1.5672 | CE 18:2 A1 | 666.6174 | [M+NH4]+ | C45H76O2 | CE 18:2 | ST |
1.8587 | CE 18:2 A2 | 671.5727 | [M+Na]+ | |||||
2.0131 | CE 18:2 A3 | 1320.1560 | [2M+Na]+ | |||||
54 | 11.62 | 1.4854 | TG 48:0 A1 | 824.7697 | [M+NH4]+ | C51H98O6 | TG 48:0 | GL |
55 | 11.65 | 1.0435 | TG 58:2 | 960.8947 | [M+NH4]+ | C61H114O6 | TG 58:2 | GL |
56 | 11.79 | 1.0106 | TG 50:0 | 852.7998 | [M+NH4]+ | C53H102O6 | TG 50:0 | GL |
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Jadranin, M.; Avramović, N.; Miladinović, Z.; Gavrilović, A.; Tasic, L.; Tešević, V.; Mandić, B. Untargeted Lipidomics Study of Bipolar Disorder Patients in Serbia. Int. J. Mol. Sci. 2023, 24, 16025. https://doi.org/10.3390/ijms242216025
Jadranin M, Avramović N, Miladinović Z, Gavrilović A, Tasic L, Tešević V, Mandić B. Untargeted Lipidomics Study of Bipolar Disorder Patients in Serbia. International Journal of Molecular Sciences. 2023; 24(22):16025. https://doi.org/10.3390/ijms242216025
Chicago/Turabian StyleJadranin, Milka, Nataša Avramović, Zoran Miladinović, Aleksandra Gavrilović, Ljubica Tasic, Vele Tešević, and Boris Mandić. 2023. "Untargeted Lipidomics Study of Bipolar Disorder Patients in Serbia" International Journal of Molecular Sciences 24, no. 22: 16025. https://doi.org/10.3390/ijms242216025