Synergic Action of Systemic Risedronate and Local Rutherpy in Peri-implantar Repair of Ovariectomized Rats: Biomechanical and Molecular Analysis
Abstract
:1. Introduction
2. Results
2.1. Clinical Data
2.2. Biomechanical Analysis (Counter-Torque)
2.3. Molecular Analysis (Real-Time PCR)
3. Discussion
4. Methods and Materials
4.1. Animals
4.2. High-Fat Diet
4.3. Clinical Data Collection
4.4. Fictitious Surgery and Bilateral Ovariectomy Surgery
4.5. Systemic and Local Drug Treatments
4.5.1. Systemic Treatment with Risedronate Sodium
4.5.2. Implant Surface Functionalization with TERPY
4.6. Implant Installation
4.7. Euthanasia
4.8. Proposed Analyses
4.8.1. Biomechanical Analysis (Removal Torque)
4.8.2. Molecular Analysis (Real-Time PCR)
5. Conclusions
6. Patents
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Groups | Type of Implant | Description |
---|---|---|
SHAM | CONV (n = 8) | Rats subjected to fictitious ovariectomy surgery |
TERPY 10 μM (n = 8) | ||
TERPY 100 μM (n = 8) | ||
OVX + HD | CONV (n = 8) | Rats subjected to ovariectomy surgery and hypercaloric diet |
TERPY 10 μM (n = 8) | ||
TERPY 100 μM (n = 8) | ||
OVX + HD + RIS | CONV (n = 8) | Rats subjected to ovariectomy surgery and hypercaloric diet and treated with sodium risedronate 0.35 mg/kg |
TERPY 10 μM (n = 8) | ||
TERPY100 μM (n = 8) |
Food | Quantity (g/rat/Day) |
---|---|
Stuffed Cracker | 10 |
Wafer | 10 |
Corn Chips | 10 |
Water + sucrose (12%) | 24 g + 200 mL of water |
Gene | Gene Name | Identification |
---|---|---|
OPG | Tnfrsf11b | Rn00563499_m1 |
RANKL | Tnfrsf11 | Rn00589289_m1 |
ALP | ALPL | Rn00564931_m1 |
IBSP | IBSP | Rn00561414_m1 |
VEGF | VEGFA | Rn01511602_m1 |
B2M | B2M | Rn00560865_m1 |
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Inoue, B.K.N.; Paludetto, L.V.; Monteiro, N.G.; Batista, F.R.d.S.; Kitagawa, I.L.; da Silva, R.S.; Antoniali, C.; Lisboa Filho, P.N.; Okamoto, R. Synergic Action of Systemic Risedronate and Local Rutherpy in Peri-implantar Repair of Ovariectomized Rats: Biomechanical and Molecular Analysis. Int. J. Mol. Sci. 2023, 24, 16153. https://doi.org/10.3390/ijms242216153
Inoue BKN, Paludetto LV, Monteiro NG, Batista FRdS, Kitagawa IL, da Silva RS, Antoniali C, Lisboa Filho PN, Okamoto R. Synergic Action of Systemic Risedronate and Local Rutherpy in Peri-implantar Repair of Ovariectomized Rats: Biomechanical and Molecular Analysis. International Journal of Molecular Sciences. 2023; 24(22):16153. https://doi.org/10.3390/ijms242216153
Chicago/Turabian StyleInoue, Bruna Kaori Namba, Laura Vidoto Paludetto, Naara Gabriela Monteiro, Fábio Roberto de Souza Batista, Igor Lebedenco Kitagawa, Roberto Santana da Silva, Cristina Antoniali, Paulo Noronha Lisboa Filho, and Roberta Okamoto. 2023. "Synergic Action of Systemic Risedronate and Local Rutherpy in Peri-implantar Repair of Ovariectomized Rats: Biomechanical and Molecular Analysis" International Journal of Molecular Sciences 24, no. 22: 16153. https://doi.org/10.3390/ijms242216153