Multiomics of GCN4-Dependent Replicative Lifespan Extension Models Reveals Gcn4 as a Regulator of Protein Turnover in Yeast
Abstract
:1. Introduction
2. Results
2.1. GCN4 Impacts Proteins Related to Amino Acid Biosynthesis in Yeast
2.2. GCN4 Impacts the Transcription of Protein Degradation Mechanisms in Yeast
2.3. Conserved Genes Involved in Protein Degradation Are Differentially Expressed in This Yeast Dataset
3. Discussion
4. Materials and Methods
4.1. Yeast Culture
4.2. Protein Extraction, Mass Spectrometry, and Proteome Analysis
4.3. RNA Extraction, Sequencing, and Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Mariner, B.L.; Felker, D.P.; Cantergiani, R.J.; Peterson, J.; McCormick, M.A. Multiomics of GCN4-Dependent Replicative Lifespan Extension Models Reveals Gcn4 as a Regulator of Protein Turnover in Yeast. Int. J. Mol. Sci. 2023, 24, 16163. https://doi.org/10.3390/ijms242216163
Mariner BL, Felker DP, Cantergiani RJ, Peterson J, McCormick MA. Multiomics of GCN4-Dependent Replicative Lifespan Extension Models Reveals Gcn4 as a Regulator of Protein Turnover in Yeast. International Journal of Molecular Sciences. 2023; 24(22):16163. https://doi.org/10.3390/ijms242216163
Chicago/Turabian StyleMariner, Blaise L., Daniel P. Felker, Ryla J. Cantergiani, Jack Peterson, and Mark A. McCormick. 2023. "Multiomics of GCN4-Dependent Replicative Lifespan Extension Models Reveals Gcn4 as a Regulator of Protein Turnover in Yeast" International Journal of Molecular Sciences 24, no. 22: 16163. https://doi.org/10.3390/ijms242216163