Transcriptomic Analysis of the Reduction in Seed Oil Content through Increased Nitrogen Application Rate in Rapeseed (Brassica napus L.)
Abstract
:1. Introduction
2. Results
2.1. Effects of Nitrogen Addition on Seed Yield, Oil and Protein Contents, Seed Total N Contents, and Seed Oil and Protein Yield
2.2. Effects of Nitrogen Addition Rates on Seed Oil Body and Protein Body Development at Different Sampling Times
2.3. Transcriptomic Analysis of Rapeseed Seeds in Three Development Stages under Three Nitrogen Levels
2.4. Analysis of Relative Gene Expression on the Pathway before Fatty Acid Metabolism
2.5. Identification of Fatty Acid Metabolism Based on qRT-PCR
3. Discussion
4. Materials and Methods
4.1. Experiment Locations, Plant Materials, and Field Management
4.2. Experimental Design
4.3. Measurement of Seed Yield and Seed Oil Contents
4.4. Measurement of Seed Protein Contents and Total Nitrogen Contents
4.5. Observation of the Development of Oil and Protein Bodies under a Transmission Electron Microscope
4.6. RNA Extraction, and Transcriptomic Analysis
4.7. qRT-PCR Identification
4.8. Statistics
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Hao, P.; Ren, Y.; Lin, B.; Yi, K.; Huang, L.; Li, X.; Jiang, L.; Hua, S. Transcriptomic Analysis of the Reduction in Seed Oil Content through Increased Nitrogen Application Rate in Rapeseed (Brassica napus L.). Int. J. Mol. Sci. 2023, 24, 16220. https://doi.org/10.3390/ijms242216220
Hao P, Ren Y, Lin B, Yi K, Huang L, Li X, Jiang L, Hua S. Transcriptomic Analysis of the Reduction in Seed Oil Content through Increased Nitrogen Application Rate in Rapeseed (Brassica napus L.). International Journal of Molecular Sciences. 2023; 24(22):16220. https://doi.org/10.3390/ijms242216220
Chicago/Turabian StyleHao, Pengfei, Yun Ren, Baogang Lin, Kaige Yi, Lan Huang, Xi Li, Lixi Jiang, and Shuijin Hua. 2023. "Transcriptomic Analysis of the Reduction in Seed Oil Content through Increased Nitrogen Application Rate in Rapeseed (Brassica napus L.)" International Journal of Molecular Sciences 24, no. 22: 16220. https://doi.org/10.3390/ijms242216220