The Neuroprotective Effects of Dendropanax morbifera Water Extract on Scopolamine-Induced Memory Impairment in Mice
Abstract
:1. Introduction
2. Results
2.1. Analysis of DMLS
2.2. Neuroprotection Assay
2.3. Effects DMLS on SCO-Induced Memory Impairment in the Morris Water-Maze Task
2.4. Effects DMLS on SCO-Induced Memory Impairment in the Y-Maze Task
2.5. Effects DMLS on SCO-Induced Memory Impairment in the Passive Avoidance Task
2.6. Effects DMLS on SCO-Induced Memory Impairment in the Novel Object Recognition (NOR) Test
2.7. Effects DMLS on Parameters of Acetylcholinesterase (AChE) Activity and Acetylcholine (ACh) Contents
2.8. Effects DMLS on Parameters of Oxidative Stress
3. Discussion
4. Materials and Methods
4.1. Plant Preparation
4.1.1. Plant Material and Preparation of DMLS Extract
4.1.2. Manufacturing Process and Quantitative High-Performance Liquid Chromatography (HPLC) of DMLS
4.2. In Vitro Experiment
4.2.1. Cell Line and Cell Culture
4.2.2. Neuroprotection Assay
4.3. In Vivo Experiment
4.3.1. Experimental Animals
4.3.2. Mice Grouping and Treatment
4.4. Y-Maze Test
4.5. Morris Water-Maze Test
4.6. Passive Avoidance Task
4.7. Novel Object Recognition Test
4.8. AChE Activity and Contents of ACh in Brain Tissues
4.9. Oxidative Stress Parameters
4.10. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Kim, S.B.; Ryu, H.Y.; Nam, W.; Lee, S.M.; Jang, M.R.; Kwak, Y.G.; Kang, G.I.; Song, K.S.; Lee, J.W. The Neuroprotective Effects of Dendropanax morbifera Water Extract on Scopolamine-Induced Memory Impairment in Mice. Int. J. Mol. Sci. 2023, 24, 16444. https://doi.org/10.3390/ijms242216444
Kim SB, Ryu HY, Nam W, Lee SM, Jang MR, Kwak YG, Kang GI, Song KS, Lee JW. The Neuroprotective Effects of Dendropanax morbifera Water Extract on Scopolamine-Induced Memory Impairment in Mice. International Journal of Molecular Sciences. 2023; 24(22):16444. https://doi.org/10.3390/ijms242216444
Chicago/Turabian StyleKim, Sung Bae, Hyun Yeoul Ryu, Woo Nam, So Min Lee, Mi Ran Jang, Youn Gil Kwak, Gyoo Il Kang, Kyung Seok Song, and Jae Won Lee. 2023. "The Neuroprotective Effects of Dendropanax morbifera Water Extract on Scopolamine-Induced Memory Impairment in Mice" International Journal of Molecular Sciences 24, no. 22: 16444. https://doi.org/10.3390/ijms242216444