IbINV Positively Regulates Resistance to Black Rot Disease Caused by Ceratocystis fimbriata in Sweet Potato
Abstract
:1. Introduction
2. Results
2.1. Cloning and Characterization of Sweet Potato IbINV Gene
2.2. Expression Patterns of IbINV in Various Tissues of Sweet Potato
2.3. IbINV Expression Profiles under Various Stresses
2.4. Generation of Transgenic Sweet Potato Plants with Modified IbINV Expression
2.5. Resistance to Black Rot Disease of Transgenic Sweet Potato Plants
2.6. Invertase Activity in Transgenic Sweet Potato Plants
2.7. Sugar Content in Transgenic Sweet Potato Plants
3. Discussion
4. Materials and Methods
4.1. Plant and Fungal Strains
4.2. IbINV Cloning and Phylogenic Analysis
4.3. Recombinant Plasmid Construction
4.4. Sweet Potato Transformation for Generation of Transgenic Plants
4.5. C. fimbriata Inoculation and Stress Treatment
4.6. Gene Expression Analysis
4.7. Black Rot Disease Resistance Assay
4.8. Sugar Measurement
4.9. Analysis of Invertase Activity
4.10. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
References
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Primer Name | Sequence (5′-…-3′) | Application |
---|---|---|
IbINV_F | ATGGCCGCCACCACTTCTTCCG | IbINV isolation |
IbINV_R | TTACAATTGATTGATGAAAGAG | IbINV isolation |
IbINV_attb_F | AAAAAGCAGGCTGCATGGCCGCCACCACTTCT | attB-IbINV |
IbINV_attb_R | AGAAAGCTGGGTCCAATTGATTGATGAAAGAG | attB-IbINV |
IbINV-S_attb_F | AAAAAGCAGGCTGCATATCGCAAAGGGCACC | attB-IbINV-S |
IbINV-S_attb_R | AGAAAGCTGGGTCCCAGATCTTCACCGACG | attB-IbINV-S |
RTIbINV_F | GGGGCCGTTCGGACTTCT | RT-PCR for IbINV |
RTIbINV_R | ACCGTGCTTCCATAAACCTCTT | RT-PCR for IbINV |
Actin-F | AGCAGCATGAAGATTAAGGTTGTAGCAC | Reference gene |
Actin-R | TGGAAAATTAGAAGCACTTCCTGTGAAC | Reference gene |
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Yang, D.; Bian, X.; Kim, H.S.; Jin, R.; Gao, F.; Chen, J.; Ma, J.; Tang, W.; Zhang, C.; Sun, H.; et al. IbINV Positively Regulates Resistance to Black Rot Disease Caused by Ceratocystis fimbriata in Sweet Potato. Int. J. Mol. Sci. 2023, 24, 16454. https://doi.org/10.3390/ijms242216454
Yang D, Bian X, Kim HS, Jin R, Gao F, Chen J, Ma J, Tang W, Zhang C, Sun H, et al. IbINV Positively Regulates Resistance to Black Rot Disease Caused by Ceratocystis fimbriata in Sweet Potato. International Journal of Molecular Sciences. 2023; 24(22):16454. https://doi.org/10.3390/ijms242216454
Chicago/Turabian StyleYang, Dongjing, Xiaofeng Bian, Ho Soo Kim, Rong Jin, Fangyuan Gao, Jingwei Chen, Jukui Ma, Wei Tang, Chengling Zhang, Houjun Sun, and et al. 2023. "IbINV Positively Regulates Resistance to Black Rot Disease Caused by Ceratocystis fimbriata in Sweet Potato" International Journal of Molecular Sciences 24, no. 22: 16454. https://doi.org/10.3390/ijms242216454