Liver Oxidative Status, Serum Lipids Levels after Bariatric Surgery and High-Fat, High-Sugar Diet in Animal Model of Induced Obesity
Abstract
:1. Introduction
2. Results
2.1. Antioxidant Status Markers
2.2. Cholesterol, Triglyceride (TG), and HDL Levels in the Serum
3. Discussion
4. Materials and Methods
4.1. Ethical Permission
4.2. Study Subject
4.3. Study Design
4.4. Experimental Procedures
4.4.1. Control (SHAM) and Duodenojejunal Omega Switch (DJOS) Surgery
4.4.2. Sample Collection and Preparation
4.4.3. Antioxidant Status Markers Analysis
Superoxide Dismutase (SOD) (EC 1.15.1.1) Activity
Catalase (CAT) Activity (EC 1.11.1.6)
Glutathione Peroxidase (GPx) Activity (EC 1.11.1.9)
Glutathione Reductase (GR) Activity (EC 1.8.1.7)
Glutathione-S Transferase (GST) Activity (EC 2.5.1.18)
Sulfhydryl Groups (SH) Concentration
Total Oxidant Status (TOS) and Total Antioxidant Capacity (TAC)
Malondialdehyde (MDA) Concentration
4.4.4. Cholesterol, Triglycerides (TG), and HDL Concentrations
4.5. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Surgery Type | Parameter [mg/dL] | CD/CD | CD/HFS | HFS/CD | HFS/HFS | Pdiet |
---|---|---|---|---|---|---|
DJOS | Cholesterol | 35 (33–37) | 38 (37–39) | 44 (44–45) | 55 (54–56) | <0.001 |
TG | 49 (46–49) | 57 (55–57) | 54 (52–56) | 59 (54–60) | <0.001 | |
HDL | 16 (15–17) | 20 (19–20) | 23 (22–24) | 20 (19–20) | <0.001 | |
SHAM | Cholesterol | 41 (40–41) | 63 (63–65) | 50 (50–50) | 77 (74–79) | <0.001 |
TG | 61 (60–62) | 135 (133–140) | 75 (69–78) | 149 (140–168) | <0.001 | |
HDL | 11 (11–12) | 13 (13–14) | 36 (30–41) | 12 (10–14) | <0.001 |
Surgery Type | Parameter | CD/CD vs. CD/HFS | CD/CD vs. HFS/CD | CD/CD vs. HFS/HFS | CD/HFS vs. HFS/CD | CD/HFS vs. HFS/HFS | HFS/CD vs. HFS/HFS |
---|---|---|---|---|---|---|---|
DJOS | Cholesterol | 1 | <0.05 | <0.001 | 0.384 | <0.01 | 0.668 |
TG | <0.01 | 0.156 | <0.001 | 1 | 1 | 0.690 | |
HDL | 0.081 | <0.001 | 0.106 | 0.149 | 1 | 0.115 | |
SHAM | Cholesterol | <0.01 | 0.668 | <0.001 | 0.688 | 0.688 | <0.01 |
TG | <0.01 | 0.668 | <0.001 | 0.476 | 1 | <0.05 | |
HDL | 0.176 | <0.001 | 1 | 0.284 | 0.917 | <0.01 |
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Kazura, W.; Michalczyk, K.; Skrzep-Poloczek, B.; Chełmecka, E.; Zalejska-Fiolka, J.; Michalski, M.; Kukla, M.; Jochem, J.; Rutkowski, J.; Stygar, D. Liver Oxidative Status, Serum Lipids Levels after Bariatric Surgery and High-Fat, High-Sugar Diet in Animal Model of Induced Obesity. Int. J. Mol. Sci. 2023, 24, 16535. https://doi.org/10.3390/ijms242216535
Kazura W, Michalczyk K, Skrzep-Poloczek B, Chełmecka E, Zalejska-Fiolka J, Michalski M, Kukla M, Jochem J, Rutkowski J, Stygar D. Liver Oxidative Status, Serum Lipids Levels after Bariatric Surgery and High-Fat, High-Sugar Diet in Animal Model of Induced Obesity. International Journal of Molecular Sciences. 2023; 24(22):16535. https://doi.org/10.3390/ijms242216535
Chicago/Turabian StyleKazura, Wojciech, Katarzyna Michalczyk, Bronisława Skrzep-Poloczek, Elżbieta Chełmecka, Jolanta Zalejska-Fiolka, Marek Michalski, Michał Kukla, Jerzy Jochem, Jakub Rutkowski, and Dominika Stygar. 2023. "Liver Oxidative Status, Serum Lipids Levels after Bariatric Surgery and High-Fat, High-Sugar Diet in Animal Model of Induced Obesity" International Journal of Molecular Sciences 24, no. 22: 16535. https://doi.org/10.3390/ijms242216535