Smooth Muscle Actin as a Criterion for Gravisensitivity of Stomach and Jejunum in Laboratory Rodents
Abstract
:1. Introduction
2. Results
2.1. Routine Microscopy
2.2. Content of α-SMA in the Stomach
2.3. α-SMA Expression in Structural Components of the Jejunum
3. Discussion
4. Materials and Methods
4.1. Experimental Design
4.2. Histoprocessing
4.3. Tissue Probe Staining
4.4. Image Acquisition
4.5. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Groups of Animals | Mucosa | Muscularis Externa | ||||
---|---|---|---|---|---|---|
Analyzed Area, mm2 (M ± m) | Area of α-SMA Expression, mm2 (M ± m) | % α-SMA | Analyzed Area, mm2 (M ± m) | Area of α-SMA Expression, mm2 (M ± m) | % α-SMA | |
The stomach | ||||||
VC-SF, n = 8 | 3.86 ± 0.04 | 0.19 ± 0.01 | 4.87 | 3.12 ± 0.07 | 2.74 ± 0.06 | 87.78 |
SF, n = 5 | 3.88 ± 0.09 | 0.20 ± 0.01 | 5.28 * | 3.10 ± 0.06 | 2.32 ± 0.02 * | 74.91 * |
VC-RSF, n = 8 | 3.73 ± 0.02 | 0.20 ± 0.002 | 5.45 | 3.29 ± 0.02 | 2.81 ± 0.09 | 85.32 |
RSF, n = 5 | 3.72 ± 0.02 | 0.21 ± 0.002 | 5.59 | 2.94 ± 0.05 * | 2.35 ± 0.04 * | 79.79 |
The jejunum | ||||||
VC-SF, n = 8 | 3.71 ± 0.02 | 0.17 ± 0.002 | 4.50 | 0.57 ± 0.02 | 0.49 ± 0.01 | 85.92 |
SF, n = 5 | 3.78 ± 0.05 | 0.20 ± 0.01 * | 5.17 * | 0.58 ± 0.02 | 0.43 ± 0.001 * | 73.50 * |
VC-RSF, n = 8 | 3.73 ± 0.03 | 0.16 ± 0.01 | 4.42 | 0.60 ± 0.03 | 0.51 ± 0.03 | 84.81 |
RSF, n = 5 | 3.61 ± 0.03 | 0.19 ± 0.002 * | 5.22 * | 0.57 ± 0.03 | 0.46 ± 0.03 | 80.89 |
Groups of Animals | Mucosa | Muscularis Externa | ||||
---|---|---|---|---|---|---|
Analyzed Area, mm2 (M ± m) | Area of α-SMA Expression, mm2 (M ± m) | % α-SMA | Analyzed Area, mm2 (M ± m) | Area of α-SMA Expression, mm2 (M ± m) | % α-SMA | |
The stomach | ||||||
Vivarium control, n = 12 | 3.76 ± 0.03 | 0.21 ± 0.002 | 5.67 | 3.59 ± 0.02 | 3.24 ± 0.02 | 90.16 |
Space flight, n = 12 | 3.55 ± 0.02 | 0.20 ± 0.002 | 5.52 | 3.42 ± 0.03 | 2.59 ± 0.04 * | 75.80 * |
Synchronous ground experiment, n = 11 | 3.54 ± 0.02 | 0.19 ± 0.001 | 5.46 * | 3.57 ± 0.02 | 3.08 ± 0.02 | 86.23 |
The jejunum | ||||||
Vivarium control, n = 12 | 3.68 ± 0.02 | 0.26 ± 0.003 | 7.16 | 1.51 ± 0.02 | 1.35 ± 0.01 | 88.97 |
Space flight, n = 12 | 3.68 ± 0.02 | 0.31 ± 0.002 | 8.48 | 1.49 ± 0.01 | 1.13 ± 0.01 | 75.71 * |
Synchronous ground experiment, n = 11 | 3.65 ± 0.01 | 0.26 ± 0.005 | 7.12 * | 1.49 ± 0.02 | 1.24 ± 0.02 | 83.29 |
Groups of Animals | Mucosa | Muscularis Externa | ||||
---|---|---|---|---|---|---|
Analyzed Area, mm2 (M ± m) | Area of α-SMA Expression, mm2 (M ± m) | % α-SMA | Analyzed Area, mm2 (M ± m) | Area of α-SMA Expression, mm2 (M ± m) | % α-SMA | |
The stomach | ||||||
control group, n = 6 | 3.76 ± 0.03 | 0.21 ± 0.003 | 5.55 | 3.01 ± 0.04 | 2.66 ± 0.04 | 88.51 |
group anti-orthostatic suspension, n = 6 | 3.64 ± 0.06 | 0.16 ± 0.001 * | 4.45 | 3.49 ± 0.06 | 2.97 ± 0.04 * | 85 * |
readaptation group, n = 6 | 3.76 ± 0.06 | 0.21 ± 0.004 | 5.7 | 3.09 ± 0.03 | 2.63 ± 0.04 | 85.08 * |
The jejunum | ||||||
control group, n = 6 | 3.70 ± 0.04 | 0.17 ± 0.005 | 4.61 | 0.58 ± 0.007 | 0.49 ± 0.005 | 86.04 |
group anti-orthostatic suspension, n = 6 | 3.73 ± 0.05 | 0.15 ± 0.004 | 3.96 * | 0.57 ± 0.007 | 0.51 ± 0.007 * | 89.44 * |
readaptation group, n = 6 | 3.73 ± 0.06 | 0.17 ± 0.004 | 4.53 | 0.57 ± 0.004 | 0.50 ± 0.005 | 86.59 |
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Samoilenko, T.; Shishkina, V.; Antakova, L.; Goryushkina, Y.; Kostin, A.; Buchwalow, I.; Tiemann, M.; Atiakshin, D. Smooth Muscle Actin as a Criterion for Gravisensitivity of Stomach and Jejunum in Laboratory Rodents. Int. J. Mol. Sci. 2023, 24, 16539. https://doi.org/10.3390/ijms242216539
Samoilenko T, Shishkina V, Antakova L, Goryushkina Y, Kostin A, Buchwalow I, Tiemann M, Atiakshin D. Smooth Muscle Actin as a Criterion for Gravisensitivity of Stomach and Jejunum in Laboratory Rodents. International Journal of Molecular Sciences. 2023; 24(22):16539. https://doi.org/10.3390/ijms242216539
Chicago/Turabian StyleSamoilenko, Tatyana, Viktoriya Shishkina, Lyubov Antakova, Yelena Goryushkina, Andrey Kostin, Igor Buchwalow, Markus Tiemann, and Dmitrii Atiakshin. 2023. "Smooth Muscle Actin as a Criterion for Gravisensitivity of Stomach and Jejunum in Laboratory Rodents" International Journal of Molecular Sciences 24, no. 22: 16539. https://doi.org/10.3390/ijms242216539