The Effect of Adipocyte-Secreted Factors in Activating Focal Adhesion Kinase-Mediated Cell Signaling Pathway towards Metastasis in Breast Cancer Cells
Abstract
:1. Introduction
2. Results
2.1. Exposure to the In Vitro Fat Cell-Derived Conditioned Medium Mimicking Obesity Signaling Increased the Migratory Potential of Breast Cells
2.2. Exposure to the In Vitro Fat Cell-Derived Conditioned Medium Mimicking Obesity Increased the Invasive Potential of Breast Cells
2.3. Exposure to the In Vitro Fat Cell-Derived Conditioned Medium Mimicking Obesity Activates FAK Protein through Its Phosphorylation at Tyr 397 Position in Breast Cancer Cells
2.4. Exposure to the In Vitro Fat Cell-Derived Conditioned Medium Increases β-Catenin Stabilization in Breast Cells
2.5. Exposure to the In Vitro Fat Cell-Derived Conditioned Medium Increases MMP-9 Protein Expression in Breast Cancer Cells
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. Wound Healing Assay
4.3. Transwell™ Matrigel™ Invasion Assay
4.4. Protein Extraction and Quantification
4.5. SDS-Polyacrylamide Gel Electrophoresis and Immunoblotting
4.6. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Mubtasim, N.; Gollahon, L. The Effect of Adipocyte-Secreted Factors in Activating Focal Adhesion Kinase-Mediated Cell Signaling Pathway towards Metastasis in Breast Cancer Cells. Int. J. Mol. Sci. 2023, 24, 16605. https://doi.org/10.3390/ijms242316605
Mubtasim N, Gollahon L. The Effect of Adipocyte-Secreted Factors in Activating Focal Adhesion Kinase-Mediated Cell Signaling Pathway towards Metastasis in Breast Cancer Cells. International Journal of Molecular Sciences. 2023; 24(23):16605. https://doi.org/10.3390/ijms242316605
Chicago/Turabian StyleMubtasim, Noshin, and Lauren Gollahon. 2023. "The Effect of Adipocyte-Secreted Factors in Activating Focal Adhesion Kinase-Mediated Cell Signaling Pathway towards Metastasis in Breast Cancer Cells" International Journal of Molecular Sciences 24, no. 23: 16605. https://doi.org/10.3390/ijms242316605