Thermodynamic Characterization of the Interaction of Biofunctionalized Gold Nanoclusters with Serum Albumin Using Two- and Three-Dimensional Methods
Abstract
:1. Introduction
2. Results
2.1. Optical and Structural Properties of the Biofunctionalized AuNCs
2.2. Surface Plasmon Resonance (SPR) Spectroscopy
2.3. Measurement of Ligand Binding Energetics using Isothermal Titration Calorimetry (ITC)
2.4. Photoluminescence (PL) Spectroscopy Investigation of Albumin Fluorescence Quenching
3. Discussion
4. Materials and Methods
4.1. Chemicals
4.2. Methods
4.2.1. Surface Plasmon Resonance (SPR) Spectroscopy
4.2.2. Isothermal Titration Calorimetry (ITC)
4.2.3. Photoluminescence (PL) Spectroscopy
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Juhász, Á.; Gombár, G.; Várkonyi, E.F.; Wojnicki, M.; Ungor, D.; Csapó, E. Thermodynamic Characterization of the Interaction of Biofunctionalized Gold Nanoclusters with Serum Albumin Using Two- and Three-Dimensional Methods. Int. J. Mol. Sci. 2023, 24, 16760. https://doi.org/10.3390/ijms242316760
Juhász Á, Gombár G, Várkonyi EF, Wojnicki M, Ungor D, Csapó E. Thermodynamic Characterization of the Interaction of Biofunctionalized Gold Nanoclusters with Serum Albumin Using Two- and Three-Dimensional Methods. International Journal of Molecular Sciences. 2023; 24(23):16760. https://doi.org/10.3390/ijms242316760
Chicago/Turabian StyleJuhász, Ádám, Gyöngyi Gombár, Egon F. Várkonyi, Marek Wojnicki, Ditta Ungor, and Edit Csapó. 2023. "Thermodynamic Characterization of the Interaction of Biofunctionalized Gold Nanoclusters with Serum Albumin Using Two- and Three-Dimensional Methods" International Journal of Molecular Sciences 24, no. 23: 16760. https://doi.org/10.3390/ijms242316760