Lipopolysaccharide Impedes Bone Repair in FcγRIIB-Deficient Mice
Abstract
:1. Introduction
2. Results
2.1. LPS Increases Serum Calcium and Decreases Renal Function
2.2. FcγRIIB−/− Mice Have Delayed Mandibular Bone Regeneration That Is Further Exacerbated by Local LPS
2.3. FcγRIIB Deficiency Causes a Decrease in Tibial Bone Regeneration and Local LPS Impaired Bone Repair
2.4. Systemic LPS Alleviates Skeletal Repair in Mice with FcγRIIB Ablation
2.5. Local and Systemic LPS Reduces Skeletal Mineralization to Collagen Ratio
2.6. Decreased Osteoblast-Associated Genes and Increased Tnfsf11/Tnfrsf11b Ratio in FcγRIIB-Deficient Mice Treated with Systemic LPS
2.7. LPS-Induced Enhanced Inflammation in FcγRIIB−/− Mice
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. DNA Isolation and Genotyping
4.3. Monocortical Osseous Hole Drilling and LPS Administration
4.4. μCT Analysis
4.5. Osteoblast Culture
4.6. Osteoclast Culture
4.7. qPCR Analysis
4.8. Mineralization Assay
4.9. Biochemical Assays
4.10. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Jantaboon, S.; Sakunrangsit, N.; Toejing, P.; Leelahavanichkul, A.; Pisitkun, P.; Greenblatt, M.B.; Lotinun, S. Lipopolysaccharide Impedes Bone Repair in FcγRIIB-Deficient Mice. Int. J. Mol. Sci. 2023, 24, 16944. https://doi.org/10.3390/ijms242316944
Jantaboon S, Sakunrangsit N, Toejing P, Leelahavanichkul A, Pisitkun P, Greenblatt MB, Lotinun S. Lipopolysaccharide Impedes Bone Repair in FcγRIIB-Deficient Mice. International Journal of Molecular Sciences. 2023; 24(23):16944. https://doi.org/10.3390/ijms242316944
Chicago/Turabian StyleJantaboon, Sirikanda, Nithidol Sakunrangsit, Parichart Toejing, Asada Leelahavanichkul, Prapaporn Pisitkun, Matthew B. Greenblatt, and Sutada Lotinun. 2023. "Lipopolysaccharide Impedes Bone Repair in FcγRIIB-Deficient Mice" International Journal of Molecular Sciences 24, no. 23: 16944. https://doi.org/10.3390/ijms242316944