Comparative Analyses of Reproductive Caste Types Reveal Vitellogenin Genes Involved in Queen Fertility in Solenopsis invicta
Abstract
:1. Introduction
2. Results
2.1. Transcriptomic Analyses
2.2. Differential Expression Analysis
2.3. Metabolic Pathways Based on KEGG Analysis
2.4. Identification and Analysis of SiVgs
2.5. Expression Profile of SiVgs
2.6. The Dynamics of SiVg Expression and Oogenesis
2.7. RNAi Effect of SiVgs
2.8. The Effects of SiVg2 and SiVg3 Knockdown on Reproduction
3. Discussion
4. Materials and Methods
4.1. Insect Rearing
4.2. RNA Sequencing
4.3. Quality Control and Read Mapping to the Reference Genome
4.4. Differential Expression and KEGG Enrichment Analysis
4.5. RNA Extraction and cDNA Synthesis
4.6. SiVg Cloning
4.7. Molecular Characterisation and Phylogenetic Analysis
4.8. qRT–PCR Analysis
4.9. Oogenesis Process of Queens
4.10. RNA Interference
4.11. Phenotypic Observation and Bioassays
4.12. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Liu, F.; Xu, F.; Zhang, Y.; Qian, Y.; Zhang, G.; Shi, L.; Peng, L. Comparative Analyses of Reproductive Caste Types Reveal Vitellogenin Genes Involved in Queen Fertility in Solenopsis invicta. Int. J. Mol. Sci. 2023, 24, 17130. https://doi.org/10.3390/ijms242417130
Liu F, Xu F, Zhang Y, Qian Y, Zhang G, Shi L, Peng L. Comparative Analyses of Reproductive Caste Types Reveal Vitellogenin Genes Involved in Queen Fertility in Solenopsis invicta. International Journal of Molecular Sciences. 2023; 24(24):17130. https://doi.org/10.3390/ijms242417130
Chicago/Turabian StyleLiu, Fenghao, Fengchao Xu, Yikun Zhang, Yurui Qian, Guofeng Zhang, Longqing Shi, and Lu Peng. 2023. "Comparative Analyses of Reproductive Caste Types Reveal Vitellogenin Genes Involved in Queen Fertility in Solenopsis invicta" International Journal of Molecular Sciences 24, no. 24: 17130. https://doi.org/10.3390/ijms242417130