Comprehensive Analysis of NAC Transcription Factors Reveals Their Evolution in Malvales and Functional Characterization of AsNAC019 and AsNAC098 in Aquilaria sinensis
Abstract
:1. Introduction
2. Results
2.1. Identification of the NAC Gene in A. sinensis and Eleven Other Species
2.2. Comparative Evolutionary Analysis of the NAC Family in Malvales
2.3. Interspecific Synteny Analysis of AsNAC and NAC from Ten Other Species
2.4. Characterization, Chromosomal Localization and Intraspecific Syntenic Analysis of the AsNAC Genes
2.5. Phylogenetic Analysis of AsNAC and AtNAC
2.6. Gene Structure and Conserved Motif Analysis of the AsNAC Gene Family
2.7. Expression Pattern of AsNAC Genes under Agarwood Inducer Treatment
2.8. Expression of AsNAC Genes under Injury Stress
2.9. Subcellular Localization of AsNAC019, AsNAC068 and AsNAC098
2.10. Transcriptional Activation of AsPKS07 by Interaction with AsNAC019, AsNAC068 and AsNAC098
3. Discussion
3.1. Identification and Evolution of NAC Transcription Factors
3.2. Potential Function of NAC Genes in A. sinensis
3.3. Possible Role of NAC Genes in Secondary Metabolite Synthesis in A. sinensis
4. Materials and Methods
4.1. Plant Material and Treatment
4.2. Identification of NAC Gene Family in the Genomes of A. sinensis and 11 Other Plants
4.3. Expansion and Contraction of NAC Genes
4.4. Identification of Syntenic Genes and Calculation of Ka, Ks and Ka/Ks Values
4.5. Phylogenetic Analysis and Chromosomal Localization of the AsNAC Transcription Factors
4.6. Gene Structure and Conserved Motif Analyses
4.7. Real-Time Quantitative PCR (RT-qPCR) Analysis
4.8. Gene Cloning and Subcellular Localization of AsNAC Genes
4.9. Yeast One-Hybrid (Y1H) Assay
4.10. Dual-Luciferase Assay
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Species | Duplication Type | Total | ||||
---|---|---|---|---|---|---|
Singleton | Dispersed | Proximal | Tandem | WGD | ||
A. trichopoda * | 44 | / | / | / | / | 44 |
V. vinifera | 1 (1%) | 36 (43%) | 6 (7%) | 6 (7%) | 35(42%) | 84 |
A. thaliana | 3 (3%) | 38 (34%) | 5 (4%) | 27 (24%) | 40 (35%) | 113 |
A. sinensis | 2 (2%) | 53 (48%) | 5 (5%) | 16 (14%) | 35 (32%) | 111 |
H. hainanensis | / | 18 (14%) | 11 (9%) | / | 98 (77%) | 127 |
D. turbinatus | / | 22 (17%) | 8 (6%) | 3 (2%) | 93 (74%) | 126 |
C. olitorius | / | 47 (58%) | 6 (7%) | 5 (6%) | 23 (28%) | 81 |
C. capsularis | 1 (1%) | 49 (57%) | 6 (7%) | 5 (6%) | 25 (29%) | 86 |
T. cacao | 2 (2%) | 40 (38%) | 23 (22%) | 12 (11%) | 28 (27%) | 105 |
D. zibethinus | 1 (1%) | 18 (8%) | 4 (2%) | 70 (31%) | 136 (59%) | 229 |
G. raimondii | 1 (1%) | 40 (26%) | 7 (5%) | 10 (7%) | 95 (62%) | 153 |
H. cannabinus | / | 38 (16%) | 23 (9%) | 21 (9%) | 161 (66%) | 243 |
Total | 55 | 399 | 104 | 175 | 769 | 1502 |
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Yang, Z.; Mei, W.; Wang, H.; Zeng, J.; Dai, H.; Ding, X. Comprehensive Analysis of NAC Transcription Factors Reveals Their Evolution in Malvales and Functional Characterization of AsNAC019 and AsNAC098 in Aquilaria sinensis. Int. J. Mol. Sci. 2023, 24, 17384. https://doi.org/10.3390/ijms242417384
Yang Z, Mei W, Wang H, Zeng J, Dai H, Ding X. Comprehensive Analysis of NAC Transcription Factors Reveals Their Evolution in Malvales and Functional Characterization of AsNAC019 and AsNAC098 in Aquilaria sinensis. International Journal of Molecular Sciences. 2023; 24(24):17384. https://doi.org/10.3390/ijms242417384
Chicago/Turabian StyleYang, Zhuo, Wenli Mei, Hao Wang, Jun Zeng, Haofu Dai, and Xupo Ding. 2023. "Comprehensive Analysis of NAC Transcription Factors Reveals Their Evolution in Malvales and Functional Characterization of AsNAC019 and AsNAC098 in Aquilaria sinensis" International Journal of Molecular Sciences 24, no. 24: 17384. https://doi.org/10.3390/ijms242417384