Effect of Low Protein Diets Supplemented with Sodium Butyrate, Medium-Chain Fatty Acids, or n-3 Polyunsaturated Fatty Acids on the Growth Performance, Immune Function, and Microbiome of Weaned Piglets
Abstract
:1. Introduction
2. Results
2.1. Growth Gerformance and Diarrhea Incidence
2.2. Plasma Immunity, Inflammatory Factors, and Jejunal Lymphocytes
2.3. Anti-Oxidation Capacity
2.4. The mRNA Expression of TLR4-IKKa and Keap1-Nrf2
2.5. Colonic Microbiome
3. Discussion
4. Materials and Methods
4.1. Experimental Design and Management
4.2. Recording and Sample Collection
4.3. Chemical Analysis
4.4. The Gene Expression in Colonic Mucosa
4.5. The Microbiome in the Colon
4.6. Statistical Analyses
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Items | Treatments 2 | SEM | p-Value | ||||
---|---|---|---|---|---|---|---|
CON | LP | LP + SB | LP + MCT | LP + PUFA | |||
Initial BW (kg) | 7.93 | 7.93 | 7.93 | 7.93 | 7.93 | 0.05 | 1.00 |
Final BW (kg) | 16.9 a | 14.1 b | 13.7 b | 13.3 b | 13.5 b | 0.27 | <0.01 |
ADG, g/d | 332 a | 229 b | 215 b | 199 b | 205 b | 10.31 | <0.01 |
ADFI, g/d | 523 a | 496 c | 517 ab | 497 c | 499 bc | 6.72 | 0.02 |
F/G, g/g | 1.58 a | 2.18 b | 2.43 b | 2.54 b | 2.48 b | 0.11 | <0.01 |
Items | Treatments 2 | SEM | p-Value | ||||
---|---|---|---|---|---|---|---|
CON | LP | LP + SB | LP + MCT | LP + PUFA | |||
Ig A (pg/mL) | 68.0 | 51.8 | 40.7 | 46.0 | 56.6 | 17.6 | 0.85 |
Ig G (pg/mL) | 4.17 | 1.79 | 1.45 | 2.32 | 3.60 | 1.18 | 0.43 |
Ig M (pg/mL) | 0.98 | 0.72 | 0.69 | 0.70 | 0.77 | 0.27 | 0.94 |
IL-6 (pg/mL) | 26.2 | 12.4 | 12.8 | 12.8 | 10.6 | 3.85 | 0.07 |
IL-8 (pg/mL) | 2.23 ab | 2.45 a | 1.14 bc | 1.25 bc | 0.85 c | 0.38 | 0.03 |
IL-10 (pg/mL) | 1.13 | 2.41 | 2.29 | 2.36 | 5.65 | 1.17 | 0.13 |
IL-17 (pg/mL) | 0.28 | 0.39 | 0.32 | 0.20 | 0.74 | 0.15 | 0.15 |
TGF-β (pg/mL) | 22.2 | 20.7 | 21.4 | 20.8 | 20.4 | 1.04 | 0.74 |
IFN-γ (pg/mL) | 2.13 | 1.19 | 1.23 | 0.92 | 0.73 | 0.35 | 0.09 |
Lymphocytes goblet cell amount | 60.8 ab | 50.6 b | 40.2 b | 86.6 a | 50.6 b | 9.35 | 0.02 |
Items | Treatments 2 | SEM | p-Value | ||||
---|---|---|---|---|---|---|---|
CON | LP | LP + SB | LP + MCT | LP + PUFA | |||
T-AOC (mM) | 1.59 | 1.56 | 1.56 | 1.53 | 1.54 | 0.03 | 0.42 |
CAT (U/mL) | 4.27 | 2.29 | 3.50 | 4.97 | 4.18 | 0.67 | 0.10 |
T-SOD (U/mL) | 37.2 | 37.9 | 38.6 | 26.6 | 28.0 | 4.67 | 0.21 |
GSH-Px (U/mL) | 522 c | 645 c | 1014 b | 1351 a | 1281 ab | 102 | <0.001 |
TNOS (U/mL) | 16.2 | 17.0 | 16.0 | 16.5 | 16.7 | 1.04 | 0.96 |
iNOS (U/mL) | 4.30 | 5.40 | 4.59 | 5.01 | 4.59 | 0.65 | 0.78 |
Items | Treatments 2 | SEM | p-Value | ||||
---|---|---|---|---|---|---|---|
CON | LP | LP + SB | LP + MCT | LP + PUFA | |||
ACE | 560 | 577 | 581 | 542 | 527 | 21.0 | 0.36 |
Chao1 | 563 | 576 | 585 | 556 | 532 | 21.6 | 0.50 |
Simpson | 0.97 | 0.98 | 0.98 | 0.97 | 0.96 | 0.004 | 0.14 |
Shannon | 6.61 | 6.81 | 6.85 | 6.54 | 6.35 | 0.14 | 0.13 |
Items (%) | Treatments 2 | SEM | p-Value | ||||
---|---|---|---|---|---|---|---|
CON | LP | LP + SB | LP + MCT | LP + PUFA | |||
Ruminococcaceae | 0.193 | 0.268 | 0.194 | 0.238 | 0.207 | 0.027 | 0.27 |
Muribaculaceae | 0.162 | 0.103 | 0.122 | 0.15 | 0.095 | 0.027 | 0.37 |
Lachnospiraceae | 0.099 | 0.118 | 0.088 | 0.077 | 0.149 | 0.024 | 0.28 |
Prevotellaceae | 0.132 | 0.079 | 0.104 | 0.085 | 0.091 | 0.018 | 0.29 |
Lactobacillaceae | 0.113 | 0.098 | 0.077 | 0.091 | 0.088 | 0.029 | 0.93 |
Rikenellaceae | 0.061 | 0.044 | 0.069 | 0.041 | 0.055 | 0.016 | 0.73 |
Spirochaetaceae | 0.042 | 0.037 | 0.06 | 0.032 | 0.064 | 0.021 | 0.74 |
Erysipelotrichaceae | 0.025 b | 0.064 a | 0.036 b | 0.038 b | 0.043 b | 0.007 | 0.01 |
Clostridiaceae-1 | 0.009 | 0.016 | 0.04 | 0.023 | 0.03 | 0.01 | 0.30 |
Christensenellaceae | 0.013 | 0.023 | 0.027 | 0.013 | 0.037 | 0.008 | 0.21 |
Acidaminococcaceae | 0.019 | 0.021 | 0.006 | 0.034 | 0.025 | 0.014 | 0.76 |
Veillonellaceae | 0.018 | 0.015 | 0.012 | 0.046 | 0.011 | 0.015 | 0.48 |
Streptococcaceae | 0.03 | 0.004 | 0.044 | 0.001 | 0.002 | 0.021 | 0.49 |
Uncultured bacterium-o- Mollicutes RF39 | 0.009 | 0.016 | 0.02 | 0.01 | 0.017 | 0.004 | 0.29 |
Family XIII | 0.01 | 0.011 | 0.013 | 0.014 | 0.013 | 0.003 | 0.90 |
Peptostreptococcaceae | 0.007 | 0.006 | 0.018 | 0.008 | 0.011 | 0.004 | 0.34 |
Tannerellaceae | 0.012 | 0.011 | 0.008 | 0.014 | 0.006 | 0.005 | 0.86 |
Items (%) | Treatments 2 | SEM | p-Value | ||||
---|---|---|---|---|---|---|---|
CON | LP | LP + SB | LP + MCT | LP + PUFA | |||
Uncultured bacterium-f-Muribaculaceae | 0.162 | 0.103 | 0.122 | 0.150 | 0.095 | 0.027 | 0.37 |
Lactobacillus | 0.113 | 0.098 | 0.077 | 0.091 | 0.088 | 0.029 | 0.93 |
Rikenellaceae-RC9-gut-group | 0.059 | 0.043 | 0.068 | 0.039 | 0.053 | 0.015 | 0.69 |
Treponema-2 | 0.038 | 0.033 | 0.055 | 0.030 | 0.060 | 0.020 | 0.75 |
Prevotellaceae-NK3B31-group | 0.045 | 0.026 | 0.040 | 0.028 | 0.036 | 0.009 | 0.58 |
Uncultured bacterium-f-Lachnospiraceae | 0.025 | 0.026 | 0.029 | 0.025 | 0.032 | 0.007 | 0.93 |
Ruminococcaceae-UCG-014 | 0.021 | 0.048 | 0.026 | 0.017 | 0.022 | 0.008 | 0.09 |
Faecalibacterium | 0.035 | 0.047 | 0.008 | 0.022 | 0.018 | 0.016 | 0.47 |
[Eubacterium]-coprostanoligenes_group | 0.021 | 0.029 | 0.019 | 0.036 | 0.021 | 0.004 | 0.08 |
Ruminococcaceae-UCG-005 | 0.013 | 0.033 | 0.027 | 0.023 | 0.029 | 0.007 | 0.39 |
Items | Treatments 1 | ||||
---|---|---|---|---|---|
CON | LP | LP + SB | LP + MCT | LP + PUFA | |
Ingredients, % | |||||
Corn | 61.45 | 69.37 | 69.23 | 69.23 | 69.23 |
Soybean meal | 12.90 | 8.30 | 8.28 | 8.28 | 8.28 |
Puffed soybean | 12.14 | 8.20 | 8.18 | 8.18 | 8.18 |
Fish meal | 4.80 | 4.70 | 4.69 | 4.69 | 4.69 |
Soybean oil | 1.80 | 1.90 | 1.90 | 1.90 | 1.90 |
Whey powder | 2.50 | 2.50 | 2.50 | 2.50 | 2.50 |
CaHPO4 | 1.10 | 1.24 | 1.24 | 1.24 | 1.24 |
Limestone powder | 0.80 | 0.80 | 0.80 | 0.80 | 0.80 |
Salt | 0.30 | 0.30 | 0.30 | 0.30 | 0.30 |
L-lysine HCl | 0.66 | 0.91 | 0.91 | 0.91 | 0.91 |
Methionine | 0.25 | 0.32 | 0.32 | 0.32 | 0.32 |
Tryptophan | 0.05 | 0.09 | 0.09 | 0.09 | 0.09 |
Threonine | 0.25 | 0.37 | 0.37 | 0.37 | 0.37 |
Premix 2 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 |
SB | 0.00 | 0.00 | 0.20 | 0.00 | 0.00 |
MCT | 0.00 | 0.00 | 0.00 | 0.20 | 0.00 |
n-3 PUFA | 0.00 | 0.00 | 0.00 | 0.00 | 0.20 |
Total | 100.00 | 100.00 | 100.00 | 100.00 | 100.00 |
Chemical composition 3 | |||||
Digestive energy DE (MJ/kg) | 14.67 | 14.58 | 14.59 | 14.59 | 14.60 |
Crude protein | 20.45 | 16.75 | 16.54 | 16.68 | 16.15 |
Ca | 0.80 | 0.89 | 0.88 | 0.87 | 0.82 |
Total P | 0.69 | 0.74 | 0.74. | 0.77 | 0.73 |
Lysine | 1.56 | 1.55 | 1.55 | 1.55 | 1.55 |
Methionine | 0.58 | 0.61 | 0.61 | 0.61 | 0.61 |
Cystine | 0.31 | 0.27 | 0.26 | 0.26 | 0.26 |
Threonine | 0.96 | 0.96 | 0.96 | 0.96 | 0.96 |
Tryptophan | 0.26 | 0.26 | 0.26 | 0.26 | 0.26 |
Arginine | 1.15 | 0.92 | 0.92 | 0.92 | 0.92 |
Histidine | 0.45 | 0.39 | 0.39 | 0.39 | 0.39 |
Isoleucine | 0.72 | 0.60 | 0.60 | 0.60 | 0.60 |
Leucine | 1.60 | 1.41 | 1.40 | 1.40 | 1.40 |
Phenylalanine | 0.81 | 0.68 | 0.67 | 0.67 | 0.67 |
Tyrosine | 0.56 | 0.49 | 0.49 | 0.49 | 0.49 |
Valine | 0.83 | 0.70 | 0.70 | 0.70 | 0.70 |
Genes 1 | Primer Sequences (5′→3′) Primer Sequence (5′→3′) | Serial Number | Tm Value (°C) | Length of PCR (bp) |
---|---|---|---|---|
TLR4 | F: CTGCCTGTGCTGAGTTTCAGGAACG R: CCTCACCCAGTCTTCGTC | NM-001293316.1 | 66 57 | 219 |
IKKα | F: AATCTGCTTCGGAACAACA R: GTCAATCTGGATGCTGGTT | XM-021077172.1 | 55 55 | 111 |
Keap1 | F: AGCAGCGGCGTTTCTACGT R: TGGGCTTGTGCAGAGTGAGC | XM_021076667.1 | 63 63 | 168 |
Nrf2 | F: TCAGACCCACCACTAGCCTT R: GTGATGCCAGCAGACCTCTT | XM_021075133.1 | 50 50 | 138 |
β-actin | F: TGCGGCATCCACCAAACTA R: CGTAGAGGTCCTTGCGGATGT | XM_021086047.1 | 57 62 | 70 |
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Li, W.; Lan, T.; Ding, Q.; Ren, Z.; Tang, Z.; Tang, Q.; Peng, X.; Xu, Y.; Sun, Z. Effect of Low Protein Diets Supplemented with Sodium Butyrate, Medium-Chain Fatty Acids, or n-3 Polyunsaturated Fatty Acids on the Growth Performance, Immune Function, and Microbiome of Weaned Piglets. Int. J. Mol. Sci. 2023, 24, 17592. https://doi.org/10.3390/ijms242417592
Li W, Lan T, Ding Q, Ren Z, Tang Z, Tang Q, Peng X, Xu Y, Sun Z. Effect of Low Protein Diets Supplemented with Sodium Butyrate, Medium-Chain Fatty Acids, or n-3 Polyunsaturated Fatty Acids on the Growth Performance, Immune Function, and Microbiome of Weaned Piglets. International Journal of Molecular Sciences. 2023; 24(24):17592. https://doi.org/10.3390/ijms242417592
Chicago/Turabian StyleLi, Wenxue, Tianyi Lan, Qi Ding, Zhongxiang Ren, Zhiru Tang, Qingsong Tang, Xie Peng, Yetong Xu, and Zhihong Sun. 2023. "Effect of Low Protein Diets Supplemented with Sodium Butyrate, Medium-Chain Fatty Acids, or n-3 Polyunsaturated Fatty Acids on the Growth Performance, Immune Function, and Microbiome of Weaned Piglets" International Journal of Molecular Sciences 24, no. 24: 17592. https://doi.org/10.3390/ijms242417592