Effects of Radioactive 56MnO2 Particle Inhalation on Mouse Lungs: A Comparison between C57BL and BALB/c
Abstract
:1. Introduction
2. Results
2.1. Estimated Absorbed Doses of Internal Irradiation in the Lungs
2.2. Body and Organ Weights
2.3. Histology of the Lung
2.4. Expression of Biodosimetry Marker Genes
2.5. Gene Expression of Aqp1, Aqp5, and Smad7
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Irradiation and Dosimetry
4.3. Measurement of mRNA Levels Using Quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR)
4.4. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Body Weights (g) | Thymus (g/kg bw) | Spleen (g/kg bw) | Lung (g/kg bw) | Heart (g/kg bw) | Liver (g/kg bw) | Kidney (g/kg bw) | Testis (g/kg bw) | |
---|---|---|---|---|---|---|---|---|
Day 3/C57BL | ||||||||
Control | 28.7 ± 1.0 | 1.7 ± 0.27 | 2.9 ± 0.30 | 10.2 ± 1.1 | 5.7 ± 0.12 | 52.5 ± 3.7 | 15.4 ± 0.58 | 6.8 ± 0.28 |
Co60 | 28.8 ± 1.2 | 0.8 ± 0.09 * | 2.8 ± 0.32 | 8.5 ± 0.73 | 5.6 ± 0.37 | 60.5 ± 3.5 | 15.6 ± 0.60 | 7.1 ± 0.64 |
Cold-Mn | 29.0 ± 0.9 | 1.4 ± 0.18 | 3.5 ± 0.30 | 9.4 ± 0.36 | 5.8 ± 0.23 | 51.9 ± 1.0 | 15.2 ± 0.33 | 6.5 ± 0.33 |
Mn56x1 | 29.5 ± 1.4 | 1.3 ± 0.08 | 4.7 ± 0.21 * | 9.0 ± 0.38 | 5.9 ± 0.18 | 55.0 ± 2.3 | 14.8 ± 0.50 | 5.9 ± 0.73 |
Mn56x3 | 29.4 ± 1.2 | 1.4 ± 0.10 | 3.3 ± 0.38 | 9.6 ± 0.55 | 4.8 ± 0.13 * | 55.2 ± 1.5 | 14.8 ± 0.82 | 6.5 ± 0.82 |
Day 3/Balb/c | ||||||||
Control | 26.3 ± 1.6 | 1.4 ± 0.14 | 3.8 ± 0.38 | 9.2 ± 0.70 | 5.6 ± 0.20 | 49.3 ± 2.5 | 17.0 ± 0.70 | 8.8 ± 0.61 |
Co60 | 26.2 ± 1.9 | 0.9 ± 0.09 * | 3.1 ± 0.40 | 8.8 ± 0.51 | 5.6 ± 0.24 | 56.1 ± 1.7 | 18.2 ± 0.52 | 9.2 ± 0.63 |
Cold-Mn | 27.5 ± 1.8 | 1.2 ± 0.11 | 4.8 ± 0.32 | 8.7 ± 0.54 | 5.5 ± 0.24 | 46.9 ± 2.7 | 17.8 ± 0.58 | 9.1 ± 0.58 |
Mn56x1 | 27.0 ± 2.1 | 1.4 ± 0.14 | 4.1 ± 0.23 | 8.8 ± 0.50 | 5.9 ± 0.21 | 52.2 ± 1.6 | 16.2 ± 0.67 | 8.5 ± 0.87 |
Mn56x3 | 27.3 ± 1.9 | 1.0 ± 0.06 * | 4.5 ± 0.53 | 9.0 ± 0.50 | 5.4 ± 0.12 | 56.9 ± 2.1 | 17.4 ± 0.50 | 7.5 ± 0.87 |
Day 65/C57BL | ||||||||
Control | 36.0 ± 1.3 | 1.6 ± 0.09 | 2.9 ± 0.28 | 7.4 ± 0.67 | 5.2 ± 0.25 | 51.1 ± 2.53 | 15.2 ± 0.59 | 5.3 ± 0.23 |
Co60 | 32.6 ± 0.6 | 1.3 ± 0.10 | 3.4 ± 0.33 | 8.3 ± 0.45 | 6.0 ± 0.36 | 48.2 ± 2.44 | 16.0 ± 0.63 | 6.6 ± 0.22 * |
Cold-Mn | 33.6 ± 1.0 | 1.4 ± 0.11 | 3.0 ± 0.29 | 7.2 ± 0.52 | 5.8 ± 0.25 | 49.2 ± 2.42 | 16.4 ± 1.06 | 6.2 ± 0.59 |
Mn56x1 | 35.4 ± 2.1 | 1.3 ± 0.14 | 3.2 ± 0.40 | 9.4 ± 0.77 | 5.5 ± 0.21 | 47.6 ± 2.5 | 14.7 ± 0.80 | 5.9 ± 0.95 |
Mn56x3 | 33.9 ± 1.5 | 1.6 ± 0.14 | 2.8 ± 0.31 | 8.3 ± 0.38 | 5.7 ± 0.33 | 43.7 ± 1.74 | 15.5 ± 0.46 | 6.9 ± 0.61 * |
Day 65/Balb/c | ||||||||
Control | 29.8 ± 2.5 | 1.2 ± 0.17 | 3.6 ± 0.22 | 8.9 ± 0.85 | 6.8 ± 1.02 | 49 ± 2.5 | 18.0 ± 0.57 | 8.7 ± 0.68 |
Co60 | 32.1 ± 2.3 | 1.2 ± 0.11 | 3.1 ± 0.28 | 7.3 ± 0.42 | 5.6 ± 0.20 | 45.9 ± 3.6 | 17.0 ± 0.94 | 7.0 ± 0.68 |
Cold-Mn | 28.6 ± 1.7 | 1.1 ± 0.09 | 3.2 ± 0.33 | 8.4 ± 0.47 | 5.6 ± 0.24 | 44.9 ± 2.1 | 17.8 ± 0.49 | 9.1 ± 0.36 |
Mn56x1 | 28.4 ± 1.6 | 1.2 ± 0.09 | 3.9 ± 0.54 | 9.7 ± 0.59 | 5.9 ± 0.31 | 49.2 ± 3.3 | 18.4 ± 0.88 | 7.1 ± 1.07 |
Mn56x3 | 27.5 ± 1.6 | 1.2 ± 0.1 | 4.0 ± 0.33 | 9.1 ± 0.8 | 6.6 ± 0.74 | 48 ± 1.4 | 19.1 ± 0.74 | 9.1 ± 0.99 |
Gene | GenBank Accession # | Q-PCR Primer Sequences (5′ -> 3′) | |
---|---|---|---|
Forward | Reverse | ||
Bax | NM_007527 | CGTGGACACGGACTCCCCCC | TGATCAGCTCGGGCACTTTA |
Phlda3 | NM_013750 | AAGCCGTGGAGTGCGTAGAG | GTCTGGATGGCCTGTTGATTC |
Faim3 | NM_026976 | TTCATGAGCAAAGGACACGC | CACCAGTCCCAAAAGAACCAG |
Aqp1 | NM_007472 | ACCTGCTGGCGATTGACTACA | CATAGATGAGCACTGCCAGGG |
Aqp5 | NM_009701 | CTCCCCAGCCTTATCCATTG | CACGATCGGTCCTACCCAGA |
Smad7 | AF015260 | TTGCTGTGAATCTTACGGGAAG | GGTTTGAGAAAATCCATTGGGT |
Actb | NM_007393.5 | CTGTCCCTGTATGCCTCTGGTC | TGAGGTAGTCCGTCAGGTCCC |
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Abishev, Z.; Ruslanova, B.; Apbassova, S.; Shabdarbayeva, D.; Chaizhunussova, N.; Dyusupov, A.; Azhimkhanov, A.; Zhumadilov, K.; Stepanenko, V.; Ivanov, S.; et al. Effects of Radioactive 56MnO2 Particle Inhalation on Mouse Lungs: A Comparison between C57BL and BALB/c. Int. J. Mol. Sci. 2023, 24, 17605. https://doi.org/10.3390/ijms242417605
Abishev Z, Ruslanova B, Apbassova S, Shabdarbayeva D, Chaizhunussova N, Dyusupov A, Azhimkhanov A, Zhumadilov K, Stepanenko V, Ivanov S, et al. Effects of Radioactive 56MnO2 Particle Inhalation on Mouse Lungs: A Comparison between C57BL and BALB/c. International Journal of Molecular Sciences. 2023; 24(24):17605. https://doi.org/10.3390/ijms242417605
Chicago/Turabian StyleAbishev, Zhaslan, Bakhyt Ruslanova, Saulesh Apbassova, Dariya Shabdarbayeva, Nailya Chaizhunussova, Altai Dyusupov, Almas Azhimkhanov, Kassym Zhumadilov, Valeriy Stepanenko, Sergey Ivanov, and et al. 2023. "Effects of Radioactive 56MnO2 Particle Inhalation on Mouse Lungs: A Comparison between C57BL and BALB/c" International Journal of Molecular Sciences 24, no. 24: 17605. https://doi.org/10.3390/ijms242417605