Three Phages One Host: Isolation and Characterization of Pantoea agglomerans Phages from a Grasshopper Specimen

Zrelovs, Nikita; Jansons, Juris; Kazaka, Tatjana; Kazaks, Andris; Dislers, Andris

doi:10.3390/ijms24031820

Open AccessArticle

Three Phages One Host: Isolation and Characterization of Pantoea agglomerans Phages from a Grasshopper Specimen

by

Nikita Zrelovs

,

Juris Jansons

,

Tatjana Kazaka

,

Andris Kazaks

^* and

Andris Dislers

^*

Latvian Biomedical Research and Study Centre, Ratsupites 1 k-1, LV-1067 Riga, Latvia

^*

Authors to whom correspondence should be addressed.

Int. J. Mol. Sci. 2023, 24(3), 1820; https://doi.org/10.3390/ijms24031820

Submission received: 20 December 2022 / Revised: 6 January 2023 / Accepted: 9 January 2023 / Published: 17 January 2023

(This article belongs to the Special Issue Bacteriophage Biology: From Genomics to Therapy)

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Abstract

The bacterial genus Pantoea comprises species found in a variety of different environmental sources. Pantoea spp. are often recovered from plant material and are capable of both benefitting the plants and acting like phytopathogens. Some species of Pantoea (including P. agglomerans) are considered opportunistic human pathogens capable of causing various infections in immunocompromised subjects. In this study, a strain of P. agglomerans (identified by 16S rRNA gene sequencing) was isolated from a dead specimen of an unidentified Latvian grasshopper species. The retrieved strain of P. agglomerans was then used as a host for the potential retrieval of phages from the same source material. After rounds of plaque purification and propagation, three high-titer lysates corresponding to putatively distinct phages were acquired. Transmission electron microscopy revealed that one of the phages was a myophage with an unusual morphology, while the two others were typical podophages. Whole-genome sequencing (WGS) was performed for each of these isolated phages. Genome de novo assembly and subsequent functional annotation confirmed that three different strictly lytic phages were isolated. Elaborate genomic characterization of the acquired phages was performed to elucidate their place within the so-far-uncovered phage diversity.

Keywords: Pantoea agglomerans; phages; insect-associated phages; podophages; myophage; whole-genome sequencing; Autographiviridae; Lietduovirus; Eracentumvirus

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MDPI and ACS Style

Zrelovs, N.; Jansons, J.; Kazaka, T.; Kazaks, A.; Dislers, A. Three Phages One Host: Isolation and Characterization of Pantoea agglomerans Phages from a Grasshopper Specimen. Int. J. Mol. Sci. 2023, 24, 1820. https://doi.org/10.3390/ijms24031820

AMA Style

Zrelovs N, Jansons J, Kazaka T, Kazaks A, Dislers A. Three Phages One Host: Isolation and Characterization of Pantoea agglomerans Phages from a Grasshopper Specimen. International Journal of Molecular Sciences. 2023; 24(3):1820. https://doi.org/10.3390/ijms24031820

Chicago/Turabian Style

Zrelovs, Nikita, Juris Jansons, Tatjana Kazaka, Andris Kazaks, and Andris Dislers. 2023. "Three Phages One Host: Isolation and Characterization of Pantoea agglomerans Phages from a Grasshopper Specimen" International Journal of Molecular Sciences 24, no. 3: 1820. https://doi.org/10.3390/ijms24031820

APA Style

Zrelovs, N., Jansons, J., Kazaka, T., Kazaks, A., & Dislers, A. (2023). Three Phages One Host: Isolation and Characterization of Pantoea agglomerans Phages from a Grasshopper Specimen. International Journal of Molecular Sciences, 24(3), 1820. https://doi.org/10.3390/ijms24031820

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Three Phages One Host: Isolation and Characterization of Pantoea agglomerans Phages from a Grasshopper Specimen

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