Proteomic Analysis of Proteins Related to Defense Responses in Arabidopsis Plants Transformed with the rolB Oncogene
Abstract
:1. Introduction
2. Results
2.1. AtB-1 Line
2.2. General Description of Proteomic Analysis
2.3. Defense Reactions and Plant Immunity
2.4. Primary Metabolism
2.5. Chaperones
2.6. Photosynthesis
2.7. RACK1-Associated Proteins
2.8. CERK1-Associated Proteins
2.9. Gene Expression
3. Discussion
3.1. Defense Response
3.2. ROS Metabolism and Abiotic Stress Response
4. Materials and Methods
4.1. Plant Material
4.2. 2D-Gel Electrophoresis
4.3. Quantification of Protein Expression
4.4. Experimental Design and Statistical Rationale
4.5. MALDI-TOF Mass Spectrometry and Protein Identification
4.6. RNA Isolation, cDNA Synthesis, and Real-Time PCR
4.6.1. RNA Isolation and cDNA Synthesis
4.6.2. Quantitative Real-Time PCR
4.6.3. Absolute Quantification
4.6.4. Statistical Analyses
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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UniProtKB Code | Name of the Protein/Short Name | Protein Function 1 | Activation, Folds 2 | |
---|---|---|---|---|
Chaperones | ||||
1 | Q38867 | Peptidyl-prolyl cis-trans isomerase CYP19-3/ROC2 | Protein peptidyl-prolyl isomerization | 3.8 |
2 | P34790 | Peptidyl-prolyl cis-trans isomerase CYP18-3/ROC1 | Protein peptidyl-prolyl isomerization | 2.0 |
3 | Q42406 | Peptidyl-prolyl cis-trans isomerase CYP18-4/ROC5 | Protein peptidyl-prolyl isomerization | 1.7 |
4 | Q38900 | Peptidyl-prolyl cis-trans isomerase CYP19-1/ROC3 | Protein peptidyl-prolyl isomerization | 1.5 |
5 | Q9LKR3 Q39043 | Heat shock 70 kDa protein BIP1/AtHsp70-11 Heat shock 70 kDa protein BIP2/AtHsp70-12 | Protein refolding; required for pollen development and pollen tube growth | 2.8 |
Plant defense | ||||
1 | P43082 | Hevein-like preproprotein/PR-4/ HEL | Defense response to bacterium, fungus, and virus; systemic acquired resistance | 10 |
2 | P50700 | Osmotin-like protein OSM34 | Defense response to fungus | 10 |
3 | Q9LJR2 | Lectin-like protein LEC | Defense response to fungus, triggered by jasmonate, ethylene and chitin | 10 |
4 | O49195 | Vegetative storage protein 1 | Defense response, response to jasmonic acid | 3.4 |
5 | O82122 | Vegetative storage protein 2 | Defense response to insects; response to wounding, oxidative stress, and jasmonic acid | 6.7 |
6 | Q9SR37 | Beta-glucosidase 23/ BGLU23/PYK10 | Major component of the ER body; glucosinolate catabolic process | 10 |
7 | Q9C5C2 | Myrosinase 2/Beta-glucosidase 37/ BGLU37 | Defense response to insect; glucosinolate catabolic process; role in abscisic acid and methyl jasmonate signaling | 2.0 |
8 | Q9SE50 | Beta-D-glucopyranosyl abscisate beta-glucosidase/BGLU18 | Defense response to fungus and insect; response to salt stress and water deprivation | 2.0 |
9 | Q9LJG3 | GDSL esterase/lipase ESM1 | Response to insect; glucosinolate catabolic process | 1.7 |
10 | O04314 | PYK10-binding protein 1 (Jacalin-related lectin 30)/PBP1 | Regulates the correct polymerization of BGLU23/PYK10 upon tissue damage | 10 |
12 | O80948 | Jacalin-related lectin 23/JAL23 | Polymerization and activation of BGLU23/PYK10 upon tissue damage | 3.3 |
13 | O04309 | Jacalin-related lectin 35/JAL35 | Component of the PYK10 complex; JA-responsive protein | 3.0 |
14 | Q9LK72 | Lectin-like protein At3g16530 | Response to oomycetes | 3.2 |
15 | Q9LNN2 | Lectin-like protein At1g53070 | May be involved in response to insect | 2.8 |
16 | Q93XW5 | Nitrile-specifier protein 5/NSP5 | Glucosinolate catabolic process, nitrile biosynthetic process | 4.5 |
17 | Q9M5J9 | Polygalacturonase inhibitor 1/PGIP-1 | Inhibitor of fungal polygalacturonase. An important factor for plant resistance to phytopathogenic fungi | 5.0 |
18 | O81862 | Class V chitinase | Hydrolyzes glycol chitin and chitin oligosaccharides; response to abscisic acid and jasmonic acid; response to salt stress | 5.1 |
19 | Q9MBH2 | Protein AIG2 B / Avirulence-induced gene 2 protein B | Response to bacterium | 4.7 |
20 | Q96262 | Plasma membrane-associated cation-binding protein 1/PCaP1 | Response to oligogalacturonides and flagellin; restricts viral cell-to-cell movement; response to cold, salt, and water deprivation | 1.7 |
21 | P32962 | Nitrilase 2/NIT2 | Involved in plant defense and R gene-mediated resistant responses to microbial pathogens | 2.0 |
Response to abiotic stress | ||||
1 | Q9SYT0 | Annexin D1/ANN1 | Response to multiple stresses, including water deprivation | 3.8 |
2 | Q0PGJ6 | NADPH-dependent aldo-keto reductase, chloroplastic | Response to cold, salt, and water deprivation | 10 |
3 | O80944 | Aldo-keto reductase family 4 member C8 | Response to cold, salt, and water deprivation | 1.5 |
4 | O49629 | Probable plastid-lipid-associated protein 2, chloroplastic/AtPap2 | Probably involved in light/cold stress-related jasmonate biosynthesis | 3.8 |
5 | P46422 | Glutathione S-transferase F2 | Regulation of binding and transport of defense-related compounds during plant stress; binds auxin, flavonoids and camalexin | 2.8 |
6 | O80852 | Glutathione S-transferase F9 | Stress response; detoxification | 2.3 |
7 | P42761 | Glutathione S-transferase F10 | Response to water deprivation | 2.0 |
8 | Q9S7E4 | Formate dehydrogenase, chloroplastic/mitochondrial/FDH | Oxidoreductase; cell stress response | 1.7 |
9 | Q9LZ66 | Assimilatory sulfite reductase (ferredoxin), chloroplastic/AtSiR | Sulfate assimilation; response to cold | 2.5 |
Photosynthesis | ||||
1 | Q9M0V6 | Ferredoxin--NADP reductase, root isozyme 1, chloroplastic /RFNR 1 | Photosynthesis; ferredoxin-NADP+ reductase activity | 4.9 |
2 | Q9LHH7 | Bifunctional protein FolD 2 | Photorespiration; one-carbon metabolic process | 2.3 |
Oxidative stress and redox homeostasis | ||||
1 | Q9SMU8 | Peroxidase 34 | Hydrogen peroxide catabolic process; response to oxidative stress; defense response to bacterium and fungus | 7.2 |
2 | Q9XEX2 | Peroxiredoxin-2B | Cell redox homeostasis, hydrogen peroxide catabolic process | 2.3 |
3 | Q9M7T0 | Peroxiredoxin-2F, mitochondrial | Cell redox homeostasis; hydrogen peroxide catabolism | 1.6 |
4 | Q949U7 | Peroxiredoxin-2E, chloroplastic | Cell redox homeostasis, hydrogen peroxide catabolic process | 1.6 |
5 | Q9FWR4 | Glutathione S-transferase DHAR1, mitochondrial | Key component of the ascorbate recycling system; involved in the redox homeostasis, especially in ROS scavenging under oxidative stress | 1.8 |
6 | Q05431 | L-ascorbate peroxidase 1, cytosolic | Key role in hydrogen peroxide removal; lignin biosynthetic process; phenylpropanoid biosynthetic process | 2.0 |
7 | P42760 | Glutathione S-transferase F6 | Response to oxidative stress; defense response to bacterium; involved in camalexin biosynthesis; response to water deprivation | 5.0 |
8 | Q9ZRW8 | Glutathione S-transferase U19 | Response to oxidative stress Response to water deprivation | 2.0 |
9 | O78310 | Superoxide dismutase [Cu-Zn] 2, chloroplastic/CSD2 | Removal of superoxide radicals; response to light intensity, UV-B; response to salt stress | 2.2 |
10 | P21276 | Superoxide dismutase [Fe] 1, chloroplastic | Removal of superoxide radicals. Response to light intensity | 1.8 |
11 | P52577 | Isoflavone reductase homolog P3 | Response to oxidative stress | 3.4 |
12 | Q9LSQ5 | NAD(P)H dehydrogenase (quinone) FQR1 | Response to oxidative stress | 2.4 |
13 | Q9SV68 | Chloroplast envelope quinone oxidoreductase homolog/ceQORH | Reduces toxic stress-related oxidized lipids produced under oxidative stress conditions | 1.8 |
Development | ||||
1 | Q9FGY1 | Beta-D-xylosidase 1 | Seed coat development | 4.4 |
2 | O65351 | Subtilisin-like protease SBT1.7 | Seed coat development | 4.7 |
3 | O64530 | Thiosulfate/3-mercaptopyruvate sulfurtransferase 1, mitochondrial/MST1 | Embryo development ending in seed dormancy | 3.9 |
4 | P24806 | Xyloglucan endotransglucosylase/hydrolase protein 24 | Cell wall biogenesis | 4.6 |
5 | P31265 | Translationally-controlled tumor protein 1/TCTP1 | General regulator required for the development of the entire plant | 1.5 |
Metabolism | ||||
1 | Q9STT3 | Aldose 1-epimerase | Carbohydrate metabolism; glucose metabolic process | 10 |
2 | A0A097PMS4 | Galactose mutarotase-like superfamily protein | Carbohydrate metabolism | 5.0 |
3 | Q9SPK5 | Formate--tetrahydrofolate ligase | One-carbon metabolism | 4.0 |
4 | Q9LYG3 | NADP-dependent malic enzyme 2 | Malate and pyruvate metabolic processes; pentose-phosphate shunt, oxidative branch | 3.0 |
5 | Q9SK66 | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial | Ubiquinone-6 biosynthetic process; electron transport | 2.9 |
6 | Q9SMN0 | Probable carboxylesterase 12 | Carboxylic ester hydrolase activity | 3.1 |
7 | Q56WD9 | 3-ketoacyl-CoA thiolase 2, peroxisomal/ PED1/KAT2 | Fatty acid biosynthesis; links fatty acid beta-oxidation with redox regulation; required for the accumulation of benzoylated glucosinolates | 3.4 |
8 | O48917 | UDP-sulfoquinovose synthase, chloroplastic | Glycolipid and sulfolipid biosynthetic processes | 3.0 |
9 | Q8VY84 | Probable UMP-CMP kinase1/UMK1 | Pyrimidine biosynthesis | 2.7 |
10 | P34066 | Proteasome subunit alpha type-1-A/PAF1 | Ubiquitin-dependent protein catabolic process | 2.5 |
11 | Q9XI05 | Proteasome subunit beta type-3-A/PBC1 | Protein catabolic process | 1.7 |
12 | Q56WN1 | Glutamine synthetase cytosolic isozyme 1-1 | Glutamine biosynthetic process | 2.6 |
13 | P47999 | Cysteine synthase, chloroplastic/chromoplastic/OASB | A major cysteine synthase | 2.2 |
14 | Q9LK23 | Glucose-6-phosphate 1-dehydrogenase 5, cytoplasmic | Pentose-phosphate shunt, oxidative branch | 2.1 |
15 | Q9FIE8 | UDP-glucuronic acid decarboxylase 3 | UDP-D-xylose biosynthetic process | 1.9 |
16 | Q9S9W2 | Short-chain dehydrogenase/reductase SDRA | Fatty acid metabolic process | 1.6 |
17 | Q9T034 | Phenylalanine--tRNA ligase alpha subunit, cytoplasmic | Protein biosynthesis | 1.5 |
18 | Q8L934 | Nucleoid DNA-binding-like protein | Proteolysis | 10 |
Other proteins | ||||
1 | Q9ZSK4 | Actin-depolymerizing factor 3/ADF-3 | Actin filament depolymerization | 4.7 |
2 | Q29Q34 | NAD(P)-binding Rossmann-fold superfamily protein / At5g19440 | Oxidoreductase activity; response to Agrobacterium tumefaciens | 1.5 |
3 | O23016 | Probable voltage-gated potassium channel subunit beta | Potassium ion transport | 1.9 |
4 | Q9XI10 | DPP6 N-terminal domain-like protein | Unknown function | 4.7 |
5 | Q8GYZ3 | RmlC-like jelly roll fold protein | Unknown function | 3.1 |
6 | Q9SSK5 | MLP-like protein 43 | Unknown function | 3.0 |
UniProtKB Code | Name of the Protein | Protein Function 1 | Inhibition, Folds 2 | |
---|---|---|---|---|
1 | O24456 | Receptor for activated C kinase 1A/ RACK1A | Major component of the RACK1 regulatory proteins that play a role in multiple signal transduction pathways | 1.5 |
2 | Q9C4Z6 | Receptor for activated C kinase 1B/RACK1B | Minor component of the RACK1 regulatory proteins | 3.8 |
3 | Q9LV28 | Receptor for activated C kinase 1C /RACK1C | Minor component of the RACK1 regulatory proteins | 4.2 |
Chaperones | ||||
1 | Q9LF37 | Chaperone protein ClpB3, chloroplastic | Molecular chaperone essential for chloroplast development and seedling viability; response to heat | 3.0 |
2 | P42730 | Chaperone protein ClpB1/Heat shock protein 101 | Molecular chaperone that plays an important role in thermotolerance | 3.0 |
3 | Q9FI56 | Chaperone protein ClpC1, chloroplastic | Regulation of chlorophyll biosynthetic process; chloroplast organization | 3.1 |
4 | Q9SAR5 | Ankyrin repeat domain-containing protein 2A | Chaperone; protein targeting to chloroplast | 2.2 |
5 | Q03250 | Glycine-rich RNA-binding protein 7/AtGR-RBP7/ GRP7 | Chaperone; innate immunity; plant defense; target of the Pseudomonas syringae type III effector HopU1 | 2.0 |
6 | P42763 | Dehydrin ERD14 | Chaperone, protein stabilization; response to cold and water deprivation | 1.9 |
7 | Q9LNB6 | Hsp70-Hsp90 organizing protein 1/AtHop1 | Mediates the association of the molecular chaperones HSP70 and HSP90; stress response | 1.5 |
Plant defense | ||||
1 | Q9FN05 | Probable glucan 1,3-alpha-glucosidase | Defense response to bacterium; required for sustained activation of EFR-mediated signaling | 3.1 |
2 | Q9FXA2 | Polyadenylate-binding protein 8/PABP-8 | Host-virus interaction | 1.7 |
3 | Q9ZUU4 | RNA-binding protein CP29B, chloroplastic | Innate immune response; potential target of HopU1 | 1.7 |
4 | Q9FGS0 | RNA-binding protein CP31B, chloroplastic | Innate immune response; potential target of HopU1 | 1.7 |
Response to stress | ||||
1 | P42759 | Dehydrin ERD10 | Protein stabilization; cold acclimation; response to abscisic acid and water deprivation | 3.9 |
2 | P54887 | Delta-1-pyrroline-5-carboxylate synthase A | Plays a key role in proline biosynthesis. Response to abscisic acid, cold, desiccation, salt stress, and oxidative stress | 2.4 |
3 | Q9FLT0 | Ribonuclease TUDOR 2 | Cytoprotective ribonuclease, which is essential for resistance to abiotic stress | 2.3 |
4 | O23523 | RGG repeats nuclear RNA binding protein A | Promotes stomata closure in drought conditions; involved in resistance to salt and drought | 1.5 |
Photosynthesis | ||||
1 | Q9SW18 | Magnesium protoporphyrin IX methyltransferase, chloroplastic | Chlorophyll biosynthesis | 3.0 |
2 | Q9LR75 | Coproporphyrinogen-III oxidase 1, chloroplastic | Chlorophyll biosynthesis | 5.0 |
3 | O22886 | Uroporphyrinogen decarboxylase 2, chloroplastic | Chlorophyll biosynthesis | 2.8 |
4 | P21218 | Protochlorophyllide reductase B, chloroplastic | Chlorophyll biosynthesis | 2.4 |
5 | O48741 | Protochlorophyllide reductase C, chloroplastic | Chlorophyll biosynthesis | 2.4 |
6 | Q9FMD5 | Protein TIC 40, chloroplastic | Chloroplast organization, protein import into chloroplast stroma | 2.3 |
7 | Q9FKW6 | Ferredoxin--NADP reductase, leaf isozyme 1, chloroplastic/FNR 1 | Photosynthetic electron transport chain | 2.0 |
8 | Q8W493 | Ferredoxin--NADP reductase, leaf isozyme 2, chloroplastic /FNR 2 | Regulates photosynthetic electron flow during the transition from dark to light | 1.7 |
9 | Q01908 | ATP synthase gamma chain 1, chloroplastic/ATPC1 | ATP biosynthetic process; photosynthetic electron transport in photosystem II | 1.8 |
10 | P42699 | Plastocyanin major isoform, chloroplastic | Electron transfer between P700 and the cytochrome b6-f complex in photosystem I | 3.0 |
11 | Q9ZR03 | Cytochrome b6-f complex iron-sulfur subunit, chloroplastic | Electron transfer between photosystem II and photosystem I | 1.5 |
12 | P37107 | Signal recognition particle 54 kDa protein, chloroplastic | Required for light-harvesting chlorophyll a/b-binding protein integration into thylakoids | 4.3 |
13 | P27140 | Beta carbonic anhydrase 1, chloroplastic/AtbCA1 | Photosynthesis; carbon utilization | 3.6 |
14 | O80796 | Membrane-associated protein VIPP1, chloroplastic | Thylakoid membrane organization | 1.9 |
General metabolism | ||||
1 | P42737 | Beta carbonic anhydrase 2, chloroplastic/AtbCA2 | Reversible hydration of carbon dioxide; plays an important role in amino acid biosynthesis | 9.8 |
2 | Q9FYE3 | Alpha carbonic anhydrase 3/AtaCA3 | Reversible hydration of carbon dioxide | 2.5 |
3 | Q9LR30 | Glutamate--glyoxylate aminotransferase 1 | Glycine biosynthetic process | 2.2 |
4 | Q56YA5 | Serine-glyoxylate aminotransferase | Glycine biosynthetic process | 1.9 |
5 | Q94B78 | Glycine dehydrogenase (decarboxylating) 1, mitochondrial | Glycine catabolic process | 2.8 |
6 | Q8W593 | Probable lactoylglutathione lyase, chloroplastic | Methylglyoxal catabolic process; response to cold | 3.3 |
7 | O50008 | 5-methyltetrahydropteroyltriglutamate homocysteine methyltransferase 1 | Methionine biosynthesis | 3.0 |
8 | Q9SYM5 | Trifunctional UDP-glucose 4,6-dehydratase/UDP-4-keto-6-deoxy-D-glucose 3,5-epimerase/UDP-4-keto-L-rhamnose-reductase RHM1 | UDP-rhamnose biosynthetic process; plays a major role in supplying UDP-rhamnose for flavonol biosynthesis | 1.7 |
9 | P56757 | ATP synthase subunit alpha, chloroplastic | ATP biosynthesis; response to cold | 3.0 |
10 | Q9C7N5 | GDSL esterase/lipase At1g29660 | Lipid metabolism | 3.0 |
11 | O23553 | Beta-amylase 3, chloroplastic | Carbohydrate metabolism; response to cold | 3.0 |
12 | Q84TF0 | Aldo-keto reductase family 4-member C10 | Metabolism of ketosteroids and aldehydes | 1.5 |
13 | Q9LD43 | Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha, chloroplastic | Fatty acid biosynthesis | 2.5 |
14 | P52410 | 3-oxoacyl-[acyl-carrier-protein] synthase I, chloroplastic | Fatty acid biosynthesis | 1.5 |
15 | P42734 | Probable cinnamyl alcohol dehydrogenase 9 | Lignin biosynthesis | 1.7 |
16 | O64767 | AICARFT/IMPCHase bienzyme family protein | Purine biosynthesis | 1.5 |
17 | Q96533 | Alcohol dehydrogenase class-3 | Ethanol oxidation; formaldehyde catabolic process | 1.6 |
18 | Q949Y0 | Ubiquitin carboxyl-terminal hydrolase 6 | Proteasome-mediated ubiquitin-dependent protein catabolic process | 3.4 |
19 | Q8H0S9 | Puromycin-sensitive aminopeptidase/PSA | Proteolysis; essential for cell growth and viability | 3.0 |
20 | Q1EBV4 | Protein DNA-DAMAGE INDUCIBLE 1 | Proteolysis | 2.3 |
Protein biosynthesis | ||||
1-2 | P49227 Q8LBI1 | 60S ribosomal protein L5-2 60S ribosomal protein L5-1 | Protein biosynthesis; leaf morphogenesis; root morphogenesis | 2.3 |
3-4 | Q9SIP7 Q9FJA6 | 40S ribosomal protein S3-1 40S ribosomal protein S3-3 | Translation | 2.3 |
5 | Q93VC7 | 30S ribosomal protein S1, chloroplastic | Translation; required for optimal plastid performance in terms of photosynthesis | 1.8 |
6 | O50061 | 50S ribosomal protein L4, chloroplastic | Translation | 1.7 |
7 | P36210 | 50S ribosomal protein L12-1, chloroplastic | Translation | 1.6 |
8 | P51412 | 50S ribosomal protein L21, chloroplastic | Translation; embryo development ending in seed dormancy | 1.8 |
9 | Q9LY66 | 50S ribosomal protein L1, chloroplastic | Translation | 1.5 |
10 | Q9SI75 | Elongation factor G, chloroplastic | Protein biosynthesis; post-embryonic development | 2.3 |
11 | Q9ASR1 | Elongation factor 2 | Protein biosynthesis; cold acclimation | 2.1 |
12 | Q8VZW6 | Elongation factor P (EF-P) family protein | Protein biosynthesis | 2.0 |
Other proteins | ||||
1 | Q941D3 | Probable plastid-lipid-associated protein 8, chloroplastic | Unknown function | 2.4 |
2 | Q8L606 | Tetratricopeptide repeat (TPR)-like superfamily protein | Unknown function | 2.9 |
3 | Q9LVT8 | RGG repeats nuclear RNA binding protein C | Unknown function | 1.5 |
UniProtKB Code | Protein | Function or Biological Process | Reference |
---|---|---|---|
Upregulated | |||
Q42406 | Peptidyl-prolyl cis-trans isomerase CYP18-4/Rotamase cyclophilin-5/ROC5 | The closest analogue AtCYP18-3 is involved in plant-pathogen interactions | [56] |
Q9ZRW8 | Glutathione S-transferase U19, cytosolic | Response to oxidative stress | [57] |
O49195 | Vegetative storage protein1/VSP1 | Defense response. Induced by mechanical wounding, jasmonic acid (JA), insect herbivory, osmotic and nutritional stresses | [40] |
Q93XW5 | Nitrile-specifier protein 5 | Defense against herbivory and pathogen attacks; component of the glucosinolate-myrosinase system | [58] |
Q9LK72 | Lectin-like protein At3g16530 | Plant defense response; homologs are induced by fungal elicitors | [59] |
Q9SR37 | Beta-glucosidase 23/PYK10 | Defense against pests and fungi | [33] |
Q9S7E4 | Formate dehydrogenase, chloroplastic/mitochondrial | Formaldehyde metabolism; induced by various stresses | [60] |
P21276 | Superoxide dismutase [Fe] 1, chloroplastic/FSD1 | Response to oxidative stress: response to light intensity | [61] |
Q9M5J9 | Polygalacturonase inhibitor 1/ PGIP1 | Restricts the growth of invasive fungal pathogens; defense against cyst nematodes | [41,42] |
Q05431 | L-ascorbate peroxidase 1, cytosolic | Cellular response to oxidative stress; phenylpropanoid biosynthetic process; lignin biosynthesis | [62] |
P24806 | Xyloglucan endotransglucosylase/ hydrolase protein 24 | Cell wall biogenesis | [63] |
Downregulated | |||
P27140 | Beta carbonic anhydrase 1, chloroplastic | Photosynthesis; stomatal development; defense response | [64] |
P36210 | 50S ribosomal protein L12-1, chloroplastic | Translation | UniProtKB |
Q9LVT8 | RGG repeats nuclear RNA binding protein C | mRNA and RNA binding | UniProtKB |
O80796 | Membrane-associated protein VIPP1, chloroplastic | Thylakoid membrane organization and vesicle organization | [65] |
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Vereshchagina, Y.V.; Mironova, A.A.; Bulgakov, D.V.; Bulgakov, V.P. Proteomic Analysis of Proteins Related to Defense Responses in Arabidopsis Plants Transformed with the rolB Oncogene. Int. J. Mol. Sci. 2023, 24, 1880. https://doi.org/10.3390/ijms24031880
Vereshchagina YV, Mironova AA, Bulgakov DV, Bulgakov VP. Proteomic Analysis of Proteins Related to Defense Responses in Arabidopsis Plants Transformed with the rolB Oncogene. International Journal of Molecular Sciences. 2023; 24(3):1880. https://doi.org/10.3390/ijms24031880
Chicago/Turabian StyleVereshchagina, Yulia V., Anastasiya A. Mironova, Dmitry V. Bulgakov, and Victor P. Bulgakov. 2023. "Proteomic Analysis of Proteins Related to Defense Responses in Arabidopsis Plants Transformed with the rolB Oncogene" International Journal of Molecular Sciences 24, no. 3: 1880. https://doi.org/10.3390/ijms24031880