Influence of Light of Different Spectral Compositions on Growth Parameters, Photosynthetic Pigment Contents and Gene Expression in Scots Pine Plantlets
Abstract
:1. Introduction
2. Results
2.1. Seed Germination and Development of Plantlets
2.2. Content of Photosynthetic Pigments
2.3. Transcript Levels of Photoreceptor Genes and TFs
2.4. Transcript Levels of Genes for Photosystems and Pigment Biosynthesis
2.5. Expression of Phytohormone Biosynthesis and Signaling Genes
3. Discussion
4. Materials and Methods
4.1. Experimental Design
4.2. Fresh Weight and Water Content
4.3. Morphometric Parameters
4.4. Determination of Photosynthetic Pigments
4.5. RNA Extraction and Quantitative RT–PCR
4.6. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Light | Seed Vigor, % | Seed Germination, % |
---|---|---|
WFL | 73.2 ± 3.3 a | 80.6 ± 4.9 a |
WL | 65.6 ± 4.7 ab | 78.4 ± 5.8 a |
BL | 55.7 ± 2.8 b | 80.2 ± 4.7 a |
RL | 58.9 ± 5.0 ab | 80.8 ± 3.3 a |
FRL | 48.1 ± 7.4 b | 81.6 ± 6.6 a |
Dark | 11.4 ± 4.3 c | 56.6 ± 3.9 b |
Light | Plantlet Length cm | Root Length cm | Hypocotyl Length cm | Cotyledons Length, cm | Plantlet Dry Weight (without Seed Coat), mg | Water Content in Plantlet, % |
---|---|---|---|---|---|---|
WFL | 5.62 ± 0.11 c | 1.37 ± 0.06 c | 2.98 ± 0.09 c | 1.24 ± 0.04 c | 3.78 ± 0.08 ab | 86.40 ± 0.21 b |
WL | 6.58 ± 0.10 b | 1.93 ± 0.08 b | 3.47 ± 0.09 b | 1.14 ± 0.03 c | 3.86 ± 0.10 ab | 87.10 ± 0.24 b |
BL | 7.27 ± 0.12 a | 1.84 ± 0.08 b | 4.03 ± 0.07 a | 1.37 ± 0.04 b | 4.43 ± 0.19 a | 86.33 ± 0.10 b |
RL | 7.42 ± 0.19 a | 2.29 ± 0.14 a | 3.49 ± 0.11 b | 1.59± 0.03 a | 4.64 ± 0.16 a | 86.60 ± 0.23 b |
FRL | 6.60 ± 0.19 b | 1.75 ± 0.08 b | 3.79± 0.13 a | 1.02± 0.04 d | 3.19 ± 0.09 b | 88.68 ± 0.13 a |
Dark | 1.89 ± 0.09 d | 1.42 ± 0.09 c | ND | ND | 3.57 ± 0.37 ab | 65.73 ± 2.08 c |
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Pashkovskiy, P.; Ivanov, Y.; Ivanova, A.; Kreslavski, V.D.; Vereshchagin, M.; Tatarkina, P.; Kuznetsov, V.V.; Allakhverdiev, S.I. Influence of Light of Different Spectral Compositions on Growth Parameters, Photosynthetic Pigment Contents and Gene Expression in Scots Pine Plantlets. Int. J. Mol. Sci. 2023, 24, 2063. https://doi.org/10.3390/ijms24032063
Pashkovskiy P, Ivanov Y, Ivanova A, Kreslavski VD, Vereshchagin M, Tatarkina P, Kuznetsov VV, Allakhverdiev SI. Influence of Light of Different Spectral Compositions on Growth Parameters, Photosynthetic Pigment Contents and Gene Expression in Scots Pine Plantlets. International Journal of Molecular Sciences. 2023; 24(3):2063. https://doi.org/10.3390/ijms24032063
Chicago/Turabian StylePashkovskiy, Pavel, Yury Ivanov, Alexandra Ivanova, Vladimir D. Kreslavski, Mikhail Vereshchagin, Polina Tatarkina, Vladimir V. Kuznetsov, and Suleyman I. Allakhverdiev. 2023. "Influence of Light of Different Spectral Compositions on Growth Parameters, Photosynthetic Pigment Contents and Gene Expression in Scots Pine Plantlets" International Journal of Molecular Sciences 24, no. 3: 2063. https://doi.org/10.3390/ijms24032063