Dendrobine Alleviates Cellular Senescence and Osteoarthritis via the ROS/NF-κB Axis
Abstract
:1. Introduction
2. Results
2.1. The Effect of DEN on the Viability of Chondrocytes
2.2. DEN Inhibited Extracellular Matrix (ECM) Degradation and Increased ECM Synthesis in IL-1β-Treated Chondrocytes
2.3. DEN Inhibited the SASP Factors Expression and Senescence Phenotype in IL-1β-Treated Chondrocytes
2.4. DEN Improved Mitochondrial Function and Reduced the Production of Intracellular ROS in IL-1β-Treated Chondrocytes
2.5. DEN Inhibited the ROS/NF-κB Pathway in IL-1β-Treated Chondrocytes
2.6. DEN Alleviates the Progression of OA in an Anterior Cruciate Ligament Transection (ACLT) Rat Model
3. Discussion
4. Materials and Methods
4.1. Chemicals and Reagents
4.2. Extraction and Culture of Chondrocytes and Cell Viability Assay
4.3. Total RNA Extraction and RT-qPCR
4.4. WB
4.5. SA-β-Gal Staining
4.6. Cellular Immunofluorescence Staining
4.7. Intracellular ROS
4.8. Mitochondrial Membrane Potential
4.9. Rat Model of OA
4.10. X-ray Images
4.11. Micro-CT
4.12. Histopathological Analysis
4.13. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
References
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Gene | Forward Primer | Reverse Primer | Annealing |
---|---|---|---|
Col2a1 | GAGTGGAAGAGCGGAGACTACTG | CTCCATGTTGCAGAAGACTTTCA | 60 °C |
Aggrecan | CTAGCTGCTTAGCAGGGATAACG | TGACCCGCAGAGTCACAAAG | 58 °C |
IL-6 | GCCAGAGTCATTCAGAGCAAT | CTTGGTCCTTAGCCACTCCT | 60 °C |
TNF-α | ACCTTATCTACTCCCAGGTTCT | GGCTGACTTTCTCCTGGTATG | 60 °C |
ADAMTS5 | GCAACAAAGTGGGACTACA | GAGAGAATGCATCCCTTAGC | 60 °C |
MMP-13 | GCAGCTCCAAAGGCTACAACTT | GTAATGGCATCAAGGGATAGGG | 60 °C |
GAPDH | GCAAGTTCAACGGCACAG | GCCAGTAGACTCCACGACA | 60 °C |
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Chen, H.; Tu, M.; Liu, S.; Wen, Y.; Chen, L. Dendrobine Alleviates Cellular Senescence and Osteoarthritis via the ROS/NF-κB Axis. Int. J. Mol. Sci. 2023, 24, 2365. https://doi.org/10.3390/ijms24032365
Chen H, Tu M, Liu S, Wen Y, Chen L. Dendrobine Alleviates Cellular Senescence and Osteoarthritis via the ROS/NF-κB Axis. International Journal of Molecular Sciences. 2023; 24(3):2365. https://doi.org/10.3390/ijms24032365
Chicago/Turabian StyleChen, Haitao, Ming Tu, Siyi Liu, Yinxian Wen, and Liaobin Chen. 2023. "Dendrobine Alleviates Cellular Senescence and Osteoarthritis via the ROS/NF-κB Axis" International Journal of Molecular Sciences 24, no. 3: 2365. https://doi.org/10.3390/ijms24032365