SOCS7-Derived BC-Box Motif Peptide Mediated Cholinergic Differentiation of Human Adipose-Derived Mesenchymal Stem Cells
Abstract
:1. Introduction
2. Results
2.1. Isolation and Proliferation of Adipose-Derived Mesenchymal Stem Cells
2.2. Characterization of ADMSCs with Flowcytometry and Immunocytochemistry
2.3. Neuronal Differentiation with SOCS7-Derived BC-Box Motif Peptide
2.4. Immunocytochemistry for ADMSCs with or without the SOCS7 Peptide Treatment
2.5. Western Blotting
2.6. Electrophysiological Analysis by Use of the Patch-Cramp Configuration
3. Discussion
4. Experimental Section
4.1. Isolation of Adipose-Tissue Derived Mesenchymal Stem Cells from Fat Tissue and Cell Culture
4.2. Flow Cytometry Analysis for Characterization of Isolated Adipose-Derived Mesenchymal Stem Cells
4.3. BC-Box Motif Peptide Design and Synthesis
4.4. Neuronal Induction with BC-Box Motif in SOCS7 Peptide
4.5. Immunocytochemistry
4.6. Western Blotting
4.7. Ubiquitination Assay
4.8. Electrophysiology with Patch-Cramp Configuration
4.9. Statistics
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Kanno, H.; Matsumoto, S.; Yoshizumi, T.; Nakahara, K.; Shinonaga, M.; Kubo, A.; Fujii, S.; Ishizuka, Y.; Tanaka, M.; Ichihashi, M.; et al. SOCS7-Derived BC-Box Motif Peptide Mediated Cholinergic Differentiation of Human Adipose-Derived Mesenchymal Stem Cells. Int. J. Mol. Sci. 2023, 24, 2786. https://doi.org/10.3390/ijms24032786
Kanno H, Matsumoto S, Yoshizumi T, Nakahara K, Shinonaga M, Kubo A, Fujii S, Ishizuka Y, Tanaka M, Ichihashi M, et al. SOCS7-Derived BC-Box Motif Peptide Mediated Cholinergic Differentiation of Human Adipose-Derived Mesenchymal Stem Cells. International Journal of Molecular Sciences. 2023; 24(3):2786. https://doi.org/10.3390/ijms24032786
Chicago/Turabian StyleKanno, Hiroshi, Shutaro Matsumoto, Tetsuya Yoshizumi, Kimihiro Nakahara, Masamichi Shinonaga, Atsuhiko Kubo, Satoshi Fujii, Yasuyuki Ishizuka, Masaki Tanaka, Masamitsu Ichihashi, and et al. 2023. "SOCS7-Derived BC-Box Motif Peptide Mediated Cholinergic Differentiation of Human Adipose-Derived Mesenchymal Stem Cells" International Journal of Molecular Sciences 24, no. 3: 2786. https://doi.org/10.3390/ijms24032786