Alanine-Dependent TCA Cycle Promotion Restores the Zhongshengmycin-Susceptibility in Xanthomonas oryzae
Abstract
:1. Introduction
2. Results
2.1. Basic Phenotypes of Z173-S and Z173-RZS
2.2. Metabolomic Analysis of Z173-S and Z173-RZS
2.3. Enrichment Pathway Analysis
2.4. The Depressed TCA Cycle Is Associated with Z173-RZS Resistance to ZSM
2.5. Inhibition of the TCA Cycle Promoted the Survival Rate of Z173-RZS under ZSM Stress
2.6. Exogenous Alanine Elevates the Sensitivity of Z173-RZS to ZSM
3. Discussion
4. Materials and Methods
4.1. Bacterial Strains and Growth Conditions
4.2. Measurement of Growth Curve
4.3. Minimum Inhibitory Concentration Detection (MIC)
4.4. Metabolomic Profiling
4.5. GC-MS Detection and Statistical Analysis
4.6. Antimicrobial Bactericidal Assays
4.7. qRT-PCR
4.8. Enzyme Activity Determination
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Zou, Z.; Lin, M.; Shen, P.; Guan, Y. Alanine-Dependent TCA Cycle Promotion Restores the Zhongshengmycin-Susceptibility in Xanthomonas oryzae. Int. J. Mol. Sci. 2023, 24, 3004. https://doi.org/10.3390/ijms24033004
Zou Z, Lin M, Shen P, Guan Y. Alanine-Dependent TCA Cycle Promotion Restores the Zhongshengmycin-Susceptibility in Xanthomonas oryzae. International Journal of Molecular Sciences. 2023; 24(3):3004. https://doi.org/10.3390/ijms24033004
Chicago/Turabian StyleZou, Zhenyu, Meiyun Lin, Peihua Shen, and Yi Guan. 2023. "Alanine-Dependent TCA Cycle Promotion Restores the Zhongshengmycin-Susceptibility in Xanthomonas oryzae" International Journal of Molecular Sciences 24, no. 3: 3004. https://doi.org/10.3390/ijms24033004