Ant-Neointimal Formation Effects of SLC6A6 in Preventing Vascular Smooth Muscle Cell Proliferation and Migration via Wnt/β-Catenin Signaling
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animal Experiments
2.2. Cell Culture
2.3. SLC6A6 Knockdown or Overexpression In Vitro
2.4. RNA Extraction and Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR)
2.5. Western Blotting
2.6. VSMC Proliferation Assay
2.7. VSMC Migration Assay
2.8. ROS Mearsurement
2.9. Human Artery Samples Collection
2.10. Histological and Morphometric Analyses
2.11. Immunohistochemical and Immunofluorescence Staining
2.12. Statistical Analysis
3. Results
3.1. SLC6A6 Decreased during VSMCs Dedifferentiation
3.2. SLC6A6 Downregulated during Neointimal Formation
3.3. SLC6A6 Decreased in Atherosclerosis
3.4. SLC6A6 Knockdown Promoted VSMCs Dedifferentiation, Proliferation, and Migration
3.5. SLC6A6 Overexpression Reduced PDGF-BB-Induced VSMC Proliferation and Migration and Dedifferentiation
3.6. SLC6A6 Regulated the Functions of VSMCs via β-Catenin Pathway
3.7. SLC6A6 Ameliorated Neointimal Formation after Vascular Injury
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Rong, Z.; Li, F.; Zhang, R.; Niu, S.; Di, X.; Ni, L.; Liu, C. Ant-Neointimal Formation Effects of SLC6A6 in Preventing Vascular Smooth Muscle Cell Proliferation and Migration via Wnt/β-Catenin Signaling. Int. J. Mol. Sci. 2023, 24, 3018. https://doi.org/10.3390/ijms24033018
Rong Z, Li F, Zhang R, Niu S, Di X, Ni L, Liu C. Ant-Neointimal Formation Effects of SLC6A6 in Preventing Vascular Smooth Muscle Cell Proliferation and Migration via Wnt/β-Catenin Signaling. International Journal of Molecular Sciences. 2023; 24(3):3018. https://doi.org/10.3390/ijms24033018
Chicago/Turabian StyleRong, Zhihua, Fengshi Li, Rui Zhang, Shuai Niu, Xiao Di, Leng Ni, and Changwei Liu. 2023. "Ant-Neointimal Formation Effects of SLC6A6 in Preventing Vascular Smooth Muscle Cell Proliferation and Migration via Wnt/β-Catenin Signaling" International Journal of Molecular Sciences 24, no. 3: 3018. https://doi.org/10.3390/ijms24033018