The EphA1 and EphA2 Signaling Modulates the Epithelial Permeability in Human Sinonasal Epithelial Cells and the Rhinovirus Infection Induces Epithelial Barrier Dysfunction via EphA2 Receptor Signaling
Abstract
:1. Introduction
2. Results
3. Discussion
4. Materials and Methods
4.1. Patients and Sampling
4.2. Epithelial Cell Culture
4.3. RV Propagation and Infection
4.4. Effect of RV Infection on Expression of EphrinA1 and EphA2 Receptor
4.5. Effect of EphrinA1/EphA2 Pathway on the Maintenance of the Epithelial Barrier Function
4.6. Small Interfering RNA (siRNA) Transfection Experiments
4.7. Western Blot
4.8. Confocal Microscopy
4.9. Statistical Analyses
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Shin, J.M.; Han, M.S.; Park, J.H.; Lee, S.H.; Kim, T.H.; Lee, S.H. The EphA1 and EphA2 Signaling Modulates the Epithelial Permeability in Human Sinonasal Epithelial Cells and the Rhinovirus Infection Induces Epithelial Barrier Dysfunction via EphA2 Receptor Signaling. Int. J. Mol. Sci. 2023, 24, 3629. https://doi.org/10.3390/ijms24043629
Shin JM, Han MS, Park JH, Lee SH, Kim TH, Lee SH. The EphA1 and EphA2 Signaling Modulates the Epithelial Permeability in Human Sinonasal Epithelial Cells and the Rhinovirus Infection Induces Epithelial Barrier Dysfunction via EphA2 Receptor Signaling. International Journal of Molecular Sciences. 2023; 24(4):3629. https://doi.org/10.3390/ijms24043629
Chicago/Turabian StyleShin, Jae Min, Moon Soo Han, Jae Hyung Park, Seung Hyeok Lee, Tae Hoon Kim, and Sang Hag Lee. 2023. "The EphA1 and EphA2 Signaling Modulates the Epithelial Permeability in Human Sinonasal Epithelial Cells and the Rhinovirus Infection Induces Epithelial Barrier Dysfunction via EphA2 Receptor Signaling" International Journal of Molecular Sciences 24, no. 4: 3629. https://doi.org/10.3390/ijms24043629