The Formation of Morphologically Stable Lipid Nanocarriers for Glioma Therapy
Abstract
:1. Introduction
2. Results
2.1. Cerasomes Obtained via Ethanol Sol Injection Method
2.2. Cerasomes Obtained via Thin Film Method
2.3. Evaluation of Cerasome Toxicity
2.4. Cerasome Uptake by T98G Cells
2.5. Analysis of Cerasome Compatibility with Blood
2.6. In Vivo Assessment of Cerasome Ability to Cross the BBB
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Cerasome Preparation
4.3. Cerasome Characterization
4.4. Cell Culture
4.5. Cytotoxicity Assessment via MTT-Assay
4.6. Induction of Apoptotic Effects
4.7. Flow Cytometry Measurement of Cell Association
4.8. Fluorescence Microscopy
4.9. In Vivo Analysis of BBB Penetration Using Confocal Microscopy of Brain Slises
4.10. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Sample Composition | Dh, nm | PdI | Zeta Potential, mV |
---|---|---|---|
100% CFL16 0% PC | 333 ± 20 | 0.300 | −20 ± 0.3 |
90% CFL16 10% PC | 252 ± 16 | 0.263 | +16.1 ± 0.7 * |
80% CFL16 20% PC | 216 ± 8 | 0.275 | −15.6 ± 0.2 |
70% CFL16 30% PC | 203 ± 2 | 0.259 | −14.6 ± 0.4 |
60% CFL16 40% PC | 191 ± 8 | 0.265 | −13.1 ± 0.2 |
50% CFL16 50% PC | 153 ± 1 | 0.262 | −12.2 ± 0.1 |
25% CFL16 75% PC | 88 ± 3 | 0.245 | −7.6 ± 0.4 |
0% CFL16 100% PC | 89 ± 8 | 0.268 | −7.0 ± 0.5 |
50% CFL16 50% PC + 1/35 14-6-14(Et) | 161 ± 8 | 0.268 | 40.7 ± 0.8 |
Sample Composition | Dh, nm | Intercept | ||||
---|---|---|---|---|---|---|
Initial | 1 h | 24 h | Initial | 1 h | 24 h | |
80% CFL16 20% PC | 216 | 188 | 258 | 0.741 | 0.743 | 0.519 |
70% CFL16 30% PC | 203 | 184 | 222 | 0.750 | 0.763 | 0.370 |
60% CFL16 40% PC | 191 | 144 | 184 | 0.706 | 0.778 | 0.490 |
50% CFL16 50% PC | 153 | 117 | 129 | 0.789 | 0.793 | 0.719 |
25% CFL16 75% PC | 88 | 102 | 190 | 0.739 | 0.537 | 0.158 |
0% CFL16 100% PC | 89 | 73 | 52,212 | 0.664 | 0.492 | 0.189 |
Sample Composition | IC50 (CFL), μM | IC50 (PTX), μM |
---|---|---|
CFL 1 mM PC 1 mM | 354.8 ± 32.7 | |
CFL 1 mM PC 1 mM Tw80 0.2 mM | 320.6 ± 29.0 | |
CFL 1 mM PC 1 mM +14-6-14(Et) | 341.2 ± 9.6 | |
PTX | 8.8 ± 0.98 | |
CFL 1 mM PC 1 mM + PTX | 2.9 ± 0.07 * | 0.24 ± 0.1 ** |
CFL 1 mM PC 1 mM Tw80 0.2 mM + PTX | 3.3 ± 0.37 * | 0.26 ± 0.04 ** |
CFL 1 mM PC 1 mM +14-6-14(Et) + PTX | 4.6 ± 0.20 * | 0.4 ± 0.04 ** |
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Pavlov, R.; Romanova, E.; Kuznetsov, D.; Lyubina, A.; Amerhanova, S.; Voloshina, A.; Buzyurova, D.; Babaev, V.; Zueva, I.; Petrov, K.; et al. The Formation of Morphologically Stable Lipid Nanocarriers for Glioma Therapy. Int. J. Mol. Sci. 2023, 24, 3632. https://doi.org/10.3390/ijms24043632
Pavlov R, Romanova E, Kuznetsov D, Lyubina A, Amerhanova S, Voloshina A, Buzyurova D, Babaev V, Zueva I, Petrov K, et al. The Formation of Morphologically Stable Lipid Nanocarriers for Glioma Therapy. International Journal of Molecular Sciences. 2023; 24(4):3632. https://doi.org/10.3390/ijms24043632
Chicago/Turabian StylePavlov, Rais, Elvira Romanova, Denis Kuznetsov, Anna Lyubina, Syumbelya Amerhanova, Alexandra Voloshina, Daina Buzyurova, Vasily Babaev, Irina Zueva, Konstantin Petrov, and et al. 2023. "The Formation of Morphologically Stable Lipid Nanocarriers for Glioma Therapy" International Journal of Molecular Sciences 24, no. 4: 3632. https://doi.org/10.3390/ijms24043632