Transcriptome Mechanisms of Tomato Seedlings Induced by Low-Red to Far-Red Light Ratio under Calcium Nitrate Stress
Abstract
:1. Introduction
2. Results
2.1. Response to Calcium Nitrate Stress
2.2. Sequencing and Transcriptome Assembly
2.3. WGCNA Analysis
2.4. RT-qPCR Verification of RNA-Seq Data
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Treatment Design
4.2. Determination of Morphological Indexes
4.2.1. Determination of Proline Metabolism, Malondialdehyde Content, and Antioxidant Enzyme Activity
4.2.2. Chlorophyll Fluorescence Parameters Determination
4.3. Transcriptome Analysis
4.4. Weighted Gene Co-Expression Network Analysis
4.5. RT-qPCR Validation
4.6. Data Processing and Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Treatment | Nutrient Solution | Light Source |
---|---|---|
CK | 1/2 Hoagland’s solution | white LED (R:FR = 4.2) |
L | 1/2 Hoagland’s solution | white LED + far-red LED (R:FR = 0.7) |
S | 1/2 Hoagland’s solution Including 160 mM of Ca(NO3)2 | white LED (R:FR = 4.2) |
SL | 1/2 Hoagland’s solution Including 160 mM of Ca(NO3)2 | white LED + far-red LED (R:FR = 0.7) |
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Zhou, X.; Huang, J.; Gan, Y.; Li, Z.; Su, L.; He, Z.; Yang, J.; Wang, Z.; Jiang, C.; Huang, Z.; et al. Transcriptome Mechanisms of Tomato Seedlings Induced by Low-Red to Far-Red Light Ratio under Calcium Nitrate Stress. Int. J. Mol. Sci. 2023, 24, 3738. https://doi.org/10.3390/ijms24043738
Zhou X, Huang J, Gan Y, Li Z, Su L, He Z, Yang J, Wang Z, Jiang C, Huang Z, et al. Transcriptome Mechanisms of Tomato Seedlings Induced by Low-Red to Far-Red Light Ratio under Calcium Nitrate Stress. International Journal of Molecular Sciences. 2023; 24(4):3738. https://doi.org/10.3390/ijms24043738
Chicago/Turabian StyleZhou, Xiaoting, Jia Huang, Yirong Gan, Zelin Li, Lihong Su, Zhongqun He, Junwei Yang, Zhihui Wang, Chengyao Jiang, Zhi Huang, and et al. 2023. "Transcriptome Mechanisms of Tomato Seedlings Induced by Low-Red to Far-Red Light Ratio under Calcium Nitrate Stress" International Journal of Molecular Sciences 24, no. 4: 3738. https://doi.org/10.3390/ijms24043738