Cyanidin-3-O-glucoside Contributes to Leaf Color Change by Regulating Two bHLH Transcription Factors in Phoebe bournei
Abstract
:1. Introduction
2. Results
2.1. Phenotypic Characteristics of P. bournei Leaves
2.2. Anthocyanin Metabolites in the Red Leaves of P. bournei at Different Developmental Stages
2.3. Identification of Key Metabolites for Leaf Coloration
2.4. Transcriptomic Analysis of Leaves during Development
2.5. DEGs Related to Anthocyanin Biosynthesis
2.6. Integrated Analysis of the Transcriptome and Metabolome
2.7. The Potential Regulatory Roles of PbbHLH1 and PbbHLH2
3. Discussion
3.1. Anthocyanin Characteristics Varied with Leaf Development in P. bournei
3.2. The Genes Involved in Anthocyanin Biosynthesis in Red Leaves of P. bournei
3.3. Transcription Factors Related to Anthocyanin Biosynthesis
4. Materials and Methods
4.1. Plant Materials
4.2. Determination of Plant Pigments
4.2.1. Determination of Total Anthocyanin Content (TAC)
4.2.2. Determination of Photosynthetic Pigments
4.3. Metabolomic Analysis of Anthocyanins
4.4. RNA Extraction, Library Construction, and Sequencing
4.5. RNA-Seq Analysis and Differentially Expressed Gene (DEG) Identification
4.6. Molecular Cloning of PbbHLH1 and PbbHLH2
4.7. Transient Expression Assay Using Nicotiana tabacum
4.8. qPCR Analysis
4.9. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Wang, L.; Wang, Q.; Fu, N.; Song, M.; Han, X.; Yang, Q.; Zhang, Y.; Tong, Z.; Zhang, J. Cyanidin-3-O-glucoside Contributes to Leaf Color Change by Regulating Two bHLH Transcription Factors in Phoebe bournei. Int. J. Mol. Sci. 2023, 24, 3829. https://doi.org/10.3390/ijms24043829
Wang L, Wang Q, Fu N, Song M, Han X, Yang Q, Zhang Y, Tong Z, Zhang J. Cyanidin-3-O-glucoside Contributes to Leaf Color Change by Regulating Two bHLH Transcription Factors in Phoebe bournei. International Journal of Molecular Sciences. 2023; 24(4):3829. https://doi.org/10.3390/ijms24043829
Chicago/Turabian StyleWang, Li, Qiguang Wang, Ningning Fu, Minyan Song, Xiao Han, Qi Yang, Yuting Zhang, Zaikang Tong, and Junhong Zhang. 2023. "Cyanidin-3-O-glucoside Contributes to Leaf Color Change by Regulating Two bHLH Transcription Factors in Phoebe bournei" International Journal of Molecular Sciences 24, no. 4: 3829. https://doi.org/10.3390/ijms24043829