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Volume 24
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10.3390/ijms24043867
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Article

Neutralising Effects of Different Antibodies on Clostridioides difficile Toxins TcdA and TcdB in a Translational Approach

by
Georg Csukovich
1,
Nina Kramer
1,
Barbara Pratscher
1,
Ivana Gotic
2,†,
Patricia Freund
1,
Rainer Hahn
3,
Gottfried Himmler
2,
Sabine Brandt
4 and
Iwan Anton Burgener
1,*
1
Small Animal Internal Medicine, Department for Companion Animals and Horses, Vetmeduni, 1210 Vienna, Austria
2
The Antibody Lab GmbH, 1210 Vienna, Austria
3
Department for Biotechnology, University of Natural Resources and Life Sciences Vienna, 1190 Vienna, Austria
4
Research Group Oncology (RGO), Clinical Unit of Equine Surgery, Department for Companion Animals and Horses, Vetmeduni, 1210 Vienna, Austria
*
Author to whom correspondence should be addressed.
†
Current address: Alterras Therapeutics GmbH, 1030 Vienna, Austria.
Int. J. Mol. Sci. 2023, 24(4), 3867; https://doi.org/10.3390/ijms24043867
Submission received: 19 December 2022 / Revised: 30 January 2023 / Accepted: 13 February 2023 / Published: 15 February 2023
(This article belongs to the Special Issue Organoids: The New 3D-Frontier to Model Different Diseases In Vitro)
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Abstract

Given the high prevalence of intestinal disease in humans and animals, there is a strong need for clinically relevant models recapitulating gastrointestinal systems, ideally replacing in vivo models in accordance with the principles of the 3R. We established a canine organoid system and analysed the neutralising effects of recombinant versus natural antibodies on Clostridioides difficile toxins A and B in this in vitro system. Sulforhodamine B cytotoxicity assays in 2D and FITC-dextran barrier integrity assays on basal-out and apical-out organoids revealed that recombinant, but not natural antibodies, effectively neutralised C. difficile toxins. Our findings emphasise that canine intestinal organoids can be used to test different components and suggest that they can be further refined to also mirror complex interactions between the intestinal epithelium and other cells.
Keywords: organoids; Clostridioides difficile; sIgA; neutralisation; toxin organoids; Clostridioides difficile; sIgA; neutralisation; toxin
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MDPI and ACS Style

Csukovich, G.; Kramer, N.; Pratscher, B.; Gotic, I.; Freund, P.; Hahn, R.; Himmler, G.; Brandt, S.; Burgener, I.A. Neutralising Effects of Different Antibodies on Clostridioides difficile Toxins TcdA and TcdB in a Translational Approach. Int. J. Mol. Sci. 2023, 24, 3867. https://doi.org/10.3390/ijms24043867

AMA Style

Csukovich G, Kramer N, Pratscher B, Gotic I, Freund P, Hahn R, Himmler G, Brandt S, Burgener IA. Neutralising Effects of Different Antibodies on Clostridioides difficile Toxins TcdA and TcdB in a Translational Approach. International Journal of Molecular Sciences. 2023; 24(4):3867. https://doi.org/10.3390/ijms24043867

Chicago/Turabian Style

Csukovich, Georg, Nina Kramer, Barbara Pratscher, Ivana Gotic, Patricia Freund, Rainer Hahn, Gottfried Himmler, Sabine Brandt, and Iwan Anton Burgener. 2023. "Neutralising Effects of Different Antibodies on Clostridioides difficile Toxins TcdA and TcdB in a Translational Approach" International Journal of Molecular Sciences 24, no. 4: 3867. https://doi.org/10.3390/ijms24043867

APA Style

Csukovich, G., Kramer, N., Pratscher, B., Gotic, I., Freund, P., Hahn, R., Himmler, G., Brandt, S., & Burgener, I. A. (2023). Neutralising Effects of Different Antibodies on Clostridioides difficile Toxins TcdA and TcdB in a Translational Approach. International Journal of Molecular Sciences, 24(4), 3867. https://doi.org/10.3390/ijms24043867

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