Changes in the Mechanical Properties of Fast and Slow Skeletal Muscle after 7 and 21 Days of Restricted Activity in Rats
Abstract
:1. Introduction
2. Results
2.1. Muscle Weight and Mechanical Characteristics
2.2. Collagen Content and mRNA Expression
2.3. MyHC mRNA Transcription
2.4. Cytoskeleton Protein Content and mRNA Transcription
3. Discussion
4. Materials and Methods
4.1. Experimental Design
4.2. Ex Vivo Muscle Mechanical Properties
4.3. Collagen Assessment
4.4. Nucleic Acids Analysis
4.5. Titin and Nebulin Electrophoresis
4.6. MyHC Immunostaining
4.7. Western Blotting
4.8. Statistcal Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene | Sequence |
---|---|
α-actinin-2 | 5′-CCGGGACTATCGTCGTAAGC-3′ |
5′-CCAGCAATGTCCGACACCAT-3′ | |
α-actinin-3 | 5′-ACTTTGACCGGAAGCGGAAT |
5′-ACCATGGTCATGATCCGAGC-3′ | |
desmin | 5′-TCTCAAGGGCACCAACGAC-3′ |
5′-GGGTGTGACATCCGAGAGTG-3′ | |
Tcap (telethonin) | 5′-CCTTCTGGGCTGAGTGGAAA-3′ |
5′-CTGCCGGTGGTAGGTCTCAT-3′ | |
collagen 1 | 5′-ATCAGCCCAAACCCCAAGGAGA-3′ |
5′-CGCAGGAAGGTCAGCTGGATAG-3′ | |
collagen 3 | 5′-TGATGGGATCCAATGAGGGAGA-3′ |
5′-GAGTCTCATGGCCTTGCGTGTTT-3′ | |
Myh7 (MyHC I) | 5′-ACAGAGGAAGACAGGAAGAACCTAC-3′ |
5′-GGGCTTCACAGGCATCCTTAG-3′ | |
Myh2 (MyHC IIa) | 5′-TATCCTCAGGCTTCAAGATTTG-3′ |
5′-TAAATAGAATCACATGGGGACA-3′ | |
Myh4 (MyHC IIb) | 5′-CTGAGGAACAATCCAACGTC-3′ |
5′-TTGTGTGATTTCTTCTGTCACCT-3′ | |
Myh1 (MyHC IId/x) | 5′-CGCGAGGTTCACACCAAA-3′ |
5′-TCCCAAAGTCGTAAGTACAAAATGG-3′ |
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Tyganov, S.A.; Belova, S.P.; Turtikova, O.V.; Vikhlyantsev, I.M.; Nemirovskaya, T.L.; Shenkman, B.S. Changes in the Mechanical Properties of Fast and Slow Skeletal Muscle after 7 and 21 Days of Restricted Activity in Rats. Int. J. Mol. Sci. 2023, 24, 4141. https://doi.org/10.3390/ijms24044141
Tyganov SA, Belova SP, Turtikova OV, Vikhlyantsev IM, Nemirovskaya TL, Shenkman BS. Changes in the Mechanical Properties of Fast and Slow Skeletal Muscle after 7 and 21 Days of Restricted Activity in Rats. International Journal of Molecular Sciences. 2023; 24(4):4141. https://doi.org/10.3390/ijms24044141
Chicago/Turabian StyleTyganov, Sergey A., Svetlana P. Belova, Olga V. Turtikova, Ivan M. Vikhlyantsev, Tatiana L. Nemirovskaya, and Boris S. Shenkman. 2023. "Changes in the Mechanical Properties of Fast and Slow Skeletal Muscle after 7 and 21 Days of Restricted Activity in Rats" International Journal of Molecular Sciences 24, no. 4: 4141. https://doi.org/10.3390/ijms24044141