Characterization and Genomic Analysis of a Novel Lytic Phage DCp1 against Clostridium perfringens Biofilms
Abstract
:1. Introduction
2. Results
2.1. Morphology of Phage DCp1
2.2. Host Range and EOP
2.3. The Optimal MOI and One-Step Growth Curve of Phage DCp1
2.4. Thermal and pH Stability of Phage DCp1
2.5. In Vitro Bactericidal Activity of Phage DCp1
2.6. Genomic Characteristics of Phage DCp1
2.7. Comparative Analysis
2.8. Phylogenetic Analysis
2.9. Effects of Phage DCp1 on the Biofilm of C. perfringens
3. Discussion
4. Materials and Methods
4.1. Strains and Conditions
4.2. Sample Enrichment and Bacteriophage Isolation
4.3. Electron Microscopy
4.4. Host Range and Efficiency of Plating (EOP) Measurement
4.5. Optimal Multiplicity of Infection (MOI)
4.6. One-Step Growth Curve
4.7. Thermal and pH Stability
4.8. In Vitro Bactericidal Activity
4.9. Genome Extraction, Sequencing, and Bioinformatics Analysis
4.10. Effects of Phage DCp1 on C. perfringens Biofilm
4.10.1. Biofilm Assay
4.10.2. Inhibitory Effect of Phage DCp1 on Biofilm Formation
4.10.3. Eradication of C. perfringens Biofilms by Phage DCp1
4.11. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Genome Characteristics | Phage | ||||||
---|---|---|---|---|---|---|---|
DCp1 | vB_CP_ qdyz_P5 | Susfortuna | CPD7 | phi24R | CPS1 | LPCPA6 | |
Genome size (bp) | 18,555 | 18,888 | 19,046 | 18,958 | 18,919 | 19,089 | 18,554 |
G + C (%) | 28.20 | 28.80 | 29.22 | 29.12 | 27.80 | 28.26 | 30.56 |
Predicted ORFs | 25 | 27 | 27 | 26 | 21 | 26 | 25 |
tRNAs | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Similarity with DCp1 (%) | 100 | 96.95 | 86.23 | 86.04 | 77.64 | 75.13 | 73.63 |
Coverage with DCp1 (%) | 100 | 85 | 74 | 74 | 0 | 1 | 21 |
Accession no. | OP256049 | OP894055.1 | NC_048712 | MK017820 | JN800508 | NC_048661 | OM638104 |
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Tang, Z.; Li, X.; Wang, X.; Zhang, C.; Zou, L.; Ren, H.; Liu, W. Characterization and Genomic Analysis of a Novel Lytic Phage DCp1 against Clostridium perfringens Biofilms. Int. J. Mol. Sci. 2023, 24, 4191. https://doi.org/10.3390/ijms24044191
Tang Z, Li X, Wang X, Zhang C, Zou L, Ren H, Liu W. Characterization and Genomic Analysis of a Novel Lytic Phage DCp1 against Clostridium perfringens Biofilms. International Journal of Molecular Sciences. 2023; 24(4):4191. https://doi.org/10.3390/ijms24044191
Chicago/Turabian StyleTang, Zhaohui, Xiaojing Li, Xinwei Wang, Can Zhang, Ling Zou, Huiying Ren, and Wenhua Liu. 2023. "Characterization and Genomic Analysis of a Novel Lytic Phage DCp1 against Clostridium perfringens Biofilms" International Journal of Molecular Sciences 24, no. 4: 4191. https://doi.org/10.3390/ijms24044191