Investigation of Glypican-4 and -6 by Infrared Spectral Imaging during the Hair Growth Cycle
Abstract
:1. Introduction
2. Results and Discussion
2.1. IR Correlation Maps Highlight the Spatial Distribution of GPC4 and GPC6 at Different Phases of the Hair Growth Cycle
2.2. Differentiation of Different Phases of the Hair Growth Cycle by GPC4 and GPC6 Expression
3. Materials and Methods
3.1. Preparation of Hair Follicle for Infrared Spectral Acquisition
3.2. Correlation of Infrared Images of HFs Using Representative Mean Spectrum of Glypican-4 and Glypican-6
3.3. Western Blot
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
GAG | glycosaminoglycan |
GPC | glypican |
GPI | glycosylphosphatidylinositol |
HF | hair follicle |
HSPG | heparan sulfate proteoglycan |
IR | infrared |
IRS | inner root sheath |
IRSI | infrared spectral imaging |
ORS | outer root sheath |
PG | proteoglycan |
TA | transit-amplifying |
References
- Malgouries, S.; Thibaut, S.; Bernard, B.A. Proteoglycan Expression Patterns in Human Hair Follicle. Br. J. Dermatol. 2008, 158, 234–242. [Google Scholar] [CrossRef]
- Bayer-Garner, I.B.; Sanderson, R.D.; Smoller, B.R. Syndecan-1 Is Strongly Expressed in the Anagen Hair Follicle Outer Root Sheath and in the Dermal Papilla but Expression Diminishes with Involution of the Hair Follicle. Am. J. Dermatopathol. 2002, 24, 484–489. [Google Scholar] [CrossRef] [PubMed]
- Colin-Pierre, C.; Untereiner, V.; Sockalingum, G.D.; Berthélémy, N.; Danoux, L.; Bardey, V.; Mine, S.; Jeanmaire, C.; Ramont, L.; Brézillon, S. Hair Histology and Glycosaminoglycans Distribution Probed by Infrared Spectral Imaging: Focus on Heparan Sulfate Proteoglycan and Glypican-1 during Hair Growth Cycle. Biomolecules 2021, 11, 192. [Google Scholar] [CrossRef] [PubMed]
- Coulson-Thomas, V.J.; Gesteira, T.F.; Esko, J.; Kao, W. Heparan Sulfate Regulates Hair Follicle and Sebaceous Gland Morphogenesis and Homeostasis. J. Biol. Chem. 2014, 289, 25211–25226. [Google Scholar] [CrossRef] [Green Version]
- Wadstein, J.; Thom, E.; Gadzhigoroeva, A. Integral Roles of Specific Proteoglycans in Hair Growth and Hair Loss: Mechanisms behind the Bioactivity of Proteoglycan Replacement Therapy with Nourkrin® with Marilex® in Pattern Hair Loss and Telogen Effluvium. Dermatol. Res. Pract. 2020, 2020, 8125081. [Google Scholar] [CrossRef] [PubMed]
- Lassus, A.; Jeskanen, L.; Happonen, H.P.; Santalahti, J. Imedeen for the Treatment of Degenerated Skin in Females. J. Int. Med. Res. 1991, 19, 147–152. [Google Scholar] [CrossRef] [PubMed]
- Thom, E. Nourkrin: Objective and Subjective Effects and Tolerability in Persons with Hair Loss. J. Int. Med. Res. 2006, 34, 514–519. [Google Scholar] [CrossRef]
- Kingsley, D.; Thorn, E. Cosmetic Hair Treatments Improve Quality of Life in Women with Female Pattern Hair Loss. J. Appl. Cosmetol. 2012, 30, 49–59. [Google Scholar]
- Karamanos, N.K.; Piperigkou, Z.; Theocharis, A.D.; Watanabe, H.; Franchi, M.; Baud, S.; Brézillon, S.; Götte, M.; Passi, A.; Vigetti, D.; et al. Proteoglycan Chemical Diversity Drives Multifunctional Cell Regulation and Therapeutics. Chem. Rev. 2018, 118, 9152–9232. [Google Scholar] [CrossRef]
- Filmus, J.; Capurro, M.; Rast, J. Glypicans. Genome Biol. 2008, 9, 224. [Google Scholar] [CrossRef] [Green Version]
- McGough, I.J.; Vecchia, L.; Bishop, B.; Malinauskas, T.; Beckett, K.; Joshi, D.; O’Reilly, N.; Siebold, C.; Jones, E.Y.; Vincent, J.-P. Glypicans Shield the Wnt Lipid Moiety to Enable Signalling at a Distance. Nature 2020, 585, 85–90. [Google Scholar] [CrossRef]
- Ayers, K.L.; Gallet, A.; Staccini-Lavenant, L.; Thérond, P.P. The Long-Range Activity of Hedgehog Is Regulated in the Apical Extracellular Space by the Glypican Dally and the Hydrolase Notum. Dev. Cell 2010, 18, 605–620. [Google Scholar] [CrossRef] [Green Version]
- Kreuger, J.; Perez, L.; Giraldez, A.J.; Cohen, S.M. Opposing Activities of Dally-like Glypican at High and Low Levels of Wingless Morphogen Activity. Dev. Cell 2004, 7, 503–512. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Pye, D.A.; Vives, R.R.; Turnbull, J.E.; Hyde, P.; Gallagher, J.T. Heparan Sulfate Oligosaccharides Require 6-O-Sulfation for Promotion of Basic Fibroblast Growth Factor Mitogenic Activity. J. Biol. Chem. 1998, 273, 22936–22942. [Google Scholar] [CrossRef] [Green Version]
- Li, N.; Spetz, M.R.; Ho, M. The Role of Glypicans in Cancer Progression and Therapy. J. Histochem. Cytochem. Off. J. Histochem. Soc. 2020, 68, 841–862. [Google Scholar] [CrossRef] [PubMed]
- Ohyama, M.; Terunuma, A.; Tock, C.L.; Radonovich, M.F.; Pise-Masison, C.A.; Hopping, S.B.; Brady, J.N.; Udey, M.C.; Vogel, J.C. Characterization and Isolation of Stem Cell-Enriched Human Hair Follicle Bulge Cells. J. Clin. Investig. 2006, 116, 249–260. [Google Scholar] [CrossRef]
- Kloepper, J.E.; Tiede, S.; Brinckmann, J.; Reinhardt, D.P.; Meyer, W.; Faessler, R.; Paus, R. Immunophenotyping of the Human Bulge Region: The Quest to Define Useful in Situ Markers for Human Epithelial Hair Follicle Stem Cells and Their Niche. Exp. Dermatol. 2008, 17, 592–609. [Google Scholar] [CrossRef] [PubMed]
- Inoue, K.; Aoi, N.; Sato, T.; Yamauchi, Y.; Suga, H.; Eto, H.; Kato, H.; Araki, J.; Yoshimura, K. Differential Expression of Stem-Cell-Associated Markers in Human Hair Follicle Epithelial Cells. Lab. Investig. 2009, 89, 844–856. [Google Scholar] [CrossRef] [Green Version]
- Purba, T.S.; Haslam, I.S.; Poblet, E.; Jiménez, F.; Gandarillas, A.; Izeta, A.; Paus, R. Human Epithelial Hair Follicle Stem Cells and Their Progeny: Current State of Knowledge, the Widening Gap in Translational Research and Future Challenges. BioEssays 2014, 36, 513–525. [Google Scholar] [CrossRef] [PubMed]
- Panteleyev, A.A. Functional Anatomy of the Hair Follicle: The Secondary Hair Germ. Exp. Dermatol. 2018, 27, 701–720. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Venero Galanternik, M.; Lush, M.E.; Piotrowski, T. Glypican4 Modulates Lateral Line Collective Cell Migration Non Cell-Autonomously. Dev. Biol. 2016, 419, 321–335. [Google Scholar] [CrossRef] [PubMed]
- Fico, A.; De Chevigny, A.; Egea, J.; Bösl, M.R.; Cremer, H.; Maina, F.; Dono, R. Modulating Glypican4 Suppresses Tumorigenicity of Embryonic Stem Cells While Preserving Self-Renewal and Pluripotency. Stem Cells 2012, 30, 1863–1874. [Google Scholar] [CrossRef] [PubMed]
- Hagihara, K.; Watanabe, K.; Chun, J.; Yamaguchi, Y. Glypican-4 Is an FGF2-Binding Heparan Sulfate Proteoglycan Expressed in Neural Precursor Cells. Dev. Dyn. Off. Publ. Am. Assoc. Anat. 2000, 219, 353–367. [Google Scholar] [CrossRef]
- Galli, A.; Roure, A.; Zeller, R.; Dono, R. Glypican 4 Modulates FGF Signalling and Regulates Dorsoventral Forebrain Patterning in Xenopus Embryos. Dev. Camb. Engl. 2003, 130, 4919–4929. [Google Scholar] [CrossRef] [Green Version]
- Müller-Röver, S.; Foitzik, K.; Paus, R.; Handjiski, B.; van der Veen, C.; Eichmüller, S.; McKay, I.A.; Stenn, K.S. A Comprehensive Guide for the Accurate Classification of Murine Hair Follicles in Distinct Hair Cycle Stages. J. Investig. Dermatol. 2001, 117, 3–15. [Google Scholar] [CrossRef] [Green Version]
- Capurro, M.; Izumikawa, T.; Suarez, P.; Shi, W.; Cydzik, M.; Kaneiwa, T.; Gariepy, J.; Bonafe, L.; Filmus, J. Glypican-6 Promotes the Growth of Developing Long Bones by Stimulating Hedgehog Signaling. J. Cell Biol. 2017, 216, 2911–2926. [Google Scholar] [CrossRef] [Green Version]
- Perrot, G.; Colin-Pierre, C.; Ramont, L.; Proult, I.; Garbar, C.; Bardey, V.; Jeanmaire, C.; Mine, S.; Danoux, L.; Berthélémy, N.; et al. Decreased Expression of GPC1 in Human Skin Keratinocytes and Epidermis during Ageing. Exp. Gerontol. 2019, 126, 110693. [Google Scholar] [CrossRef]
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Colin-Pierre, C.; Untereiner, V.; Sockalingum, G.D.; Ramont, L.; Brézillon, S. Investigation of Glypican-4 and -6 by Infrared Spectral Imaging during the Hair Growth Cycle. Int. J. Mol. Sci. 2023, 24, 4291. https://doi.org/10.3390/ijms24054291
Colin-Pierre C, Untereiner V, Sockalingum GD, Ramont L, Brézillon S. Investigation of Glypican-4 and -6 by Infrared Spectral Imaging during the Hair Growth Cycle. International Journal of Molecular Sciences. 2023; 24(5):4291. https://doi.org/10.3390/ijms24054291
Chicago/Turabian StyleColin-Pierre, Charlie, Valérie Untereiner, Ganesh D. Sockalingum, Laurent Ramont, and Stéphane Brézillon. 2023. "Investigation of Glypican-4 and -6 by Infrared Spectral Imaging during the Hair Growth Cycle" International Journal of Molecular Sciences 24, no. 5: 4291. https://doi.org/10.3390/ijms24054291