Comparative Transcriptomic Analysis Revealed the Suppression and Alternative Splicing of Kiwifruit (Actinidia latifolia) NAP1 Gene Mediating Trichome Development
Abstract
:1. Introduction
2. Results
2.1. Morphologic Observation of Trichomes in Two Kiwifruit Species
2.2. The Third- and Second-Generation Transcriptome Analysis
2.3. Alternative Splicing of AlNAP1 mRNA
2.4. Complementation of Arabidopsis nap1 Mutant Was Achieved by AlNAP1-FL but Not by AlNAP1-AS1
3. Discussion
4. Materials and Methods
4.1. Plant Material and Growth Conditions
4.2. RNA Extraction and qRT−PCR Assays
4.3. Cloning of AlNAP1 and AeNAP1
4.4. Vector Construction and Plant Transformation
4.5. Sequence Alignment and Protein Domain Analysis
4.6. Third- and Second-Generation Transcriptome Sequencing
4.7. Protein Extraction and Western Blotting
4.8. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Samples | Number of Consensus Isoforms | Average Consensus Isoforms Read Length | Number of Polished High-Quality Isoforms | Number of Polished Low-Quality Isoforms | Percent of Polished High-Quality Isoforms (%) | Non-Redundant Transcript Sequences |
---|---|---|---|---|---|---|
Al-q | 135,307 | 1844 | 135,286 | 21 | 99.98% | 126,102 |
Ae-q | 156,765 | 1522 | 156,752 | 13 | 99.99% | 93,226 |
Al_vs_Ae | Total Number | Up-Regulated | Down-Regulated |
---|---|---|---|
Differentially expressed transcripts (DETs) | 88,270 | 39,879 | 48,391 |
Differentially expressed genes (DEGs) | 12,950 | 5851 | 7099 |
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Miao, T.; Bao, H.; Ling, H.; Li, P.; Zhang, Y.; He, Y.; Hu, X.; Ling, C.; Liu, Y.; Tang, W.; et al. Comparative Transcriptomic Analysis Revealed the Suppression and Alternative Splicing of Kiwifruit (Actinidia latifolia) NAP1 Gene Mediating Trichome Development. Int. J. Mol. Sci. 2023, 24, 4481. https://doi.org/10.3390/ijms24054481
Miao T, Bao H, Ling H, Li P, Zhang Y, He Y, Hu X, Ling C, Liu Y, Tang W, et al. Comparative Transcriptomic Analysis Revealed the Suppression and Alternative Splicing of Kiwifruit (Actinidia latifolia) NAP1 Gene Mediating Trichome Development. International Journal of Molecular Sciences. 2023; 24(5):4481. https://doi.org/10.3390/ijms24054481
Chicago/Turabian StyleMiao, Tonghao, Huaxu Bao, Hui Ling, Pengwei Li, Yiling Zhang, Yan He, Xufan Hu, Chengcheng Ling, Yunyan Liu, Wei Tang, and et al. 2023. "Comparative Transcriptomic Analysis Revealed the Suppression and Alternative Splicing of Kiwifruit (Actinidia latifolia) NAP1 Gene Mediating Trichome Development" International Journal of Molecular Sciences 24, no. 5: 4481. https://doi.org/10.3390/ijms24054481