Overexpression of Potential Markers of Regulatory and Exhausted CD8+ T Cells in the Peripheral Blood Mononuclear Cells of Patients with B-Acute Lymphoblastic Leukemia
Abstract
:1. Introduction
2. Results
2.1. Treg/CD8 Exhaustion Marker Overexpression in Patients with B-ALL
2.2. Correlation of Treg/CD8 Exhaustion Marker Overexpression with Higher Leukemic Cell Proliferation Rate
2.3. Treg Phenotypes of Cells Expressing Treg/CD8 Exhaustion Markers
2.4. Treg/CD8 Exhaustion Marker Expression by Exhausted CD8+ T Cells
3. Discussion
4. Materials and Methods
4.1. Data Source, Tumor Type, and Controls
4.2. Genes of Interest
4.3. Use of T Cell Signature to Normalize Gene Expression
4.4. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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CD39 | CTLA-4 | TIGIT | TIM-3 | TNFR2 | |
---|---|---|---|---|---|
FoxP3 | 0.66 | 0.73 | 0.86 | 0.56 | ns |
Helios | ns | ns | 0.66 | ns | ns |
IL-10 | 0.62 | 0.47 | 0.52 | 0.51 | 0.43 |
TGF-β | ns | ns | ns | 0.40 | 0.66 |
TIM-3 | CD39 | TIGIT | CTLA-4 | TNFR2 | |
---|---|---|---|---|---|
mean of CD8α chain + CD8β chain | 0.50 | 0.50 | 0.56 | 0.61 | 0.58 |
mean of Granzyme B + Granulysin | −0.55 | −0.58 | −0.76 | −0.58 | ns |
(mean of CD8α chain + CD8β chain)—(mean of Granzyme B + Granulysin) | 0.68 | 0.67 | 0.81 | 0.73 | ns |
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Naghavi Alhosseini, M.; Palazzo, M.; Cari, L.; Ronchetti, S.; Migliorati, G.; Nocentini, G. Overexpression of Potential Markers of Regulatory and Exhausted CD8+ T Cells in the Peripheral Blood Mononuclear Cells of Patients with B-Acute Lymphoblastic Leukemia. Int. J. Mol. Sci. 2023, 24, 4526. https://doi.org/10.3390/ijms24054526
Naghavi Alhosseini M, Palazzo M, Cari L, Ronchetti S, Migliorati G, Nocentini G. Overexpression of Potential Markers of Regulatory and Exhausted CD8+ T Cells in the Peripheral Blood Mononuclear Cells of Patients with B-Acute Lymphoblastic Leukemia. International Journal of Molecular Sciences. 2023; 24(5):4526. https://doi.org/10.3390/ijms24054526
Chicago/Turabian StyleNaghavi Alhosseini, Mahdieh, Marianna Palazzo, Luigi Cari, Simona Ronchetti, Graziella Migliorati, and Giuseppe Nocentini. 2023. "Overexpression of Potential Markers of Regulatory and Exhausted CD8+ T Cells in the Peripheral Blood Mononuclear Cells of Patients with B-Acute Lymphoblastic Leukemia" International Journal of Molecular Sciences 24, no. 5: 4526. https://doi.org/10.3390/ijms24054526