Pseudomonas aeruginosa Dps (PA0962) Functions in H2O2 Mediated Oxidative Stress Defense and Exhibits In Vitro DNA Cleaving Activity
Abstract
:1. Introduction
2. Results and Discussion
2.1. Pa Dps Oligomerization in Solution
2.2. Pa Dps X-ray Crystal Structure
2.3. Pa Dps in Complex with Mn2+
2.4. Pa Catalyzes the Oxidation of Fe2+ When the Oxidant Is H2O2
2.5. A Network of Tyr Residues Traps Hydroxyl Radical within the Protein Shell
2.6. Pa Dps Protects P. aeruginosa Cells from H2O2-Mediated Oxidative Stress
2.7. Pa Dps Cleaves DNA In Vitro
2.8. Pa Dps Does Not Contribute to the Utilization of DNA as a Nutrient Source
3. Materials and Methods
3.1. Chemicals, Bacterial Strains, and Media
3.2. Expression and Purification of Pa Dps
3.3. Titration of Pa Dps with Fe2+ under Aerobic and Anaerobic Conditions
3.4. Proteomics Analysis of Di-Tyrosine Crosslinked Pa Dps
3.5. Crystallization and Data Collection
3.6. Structure Solution and Refinement
3.7. Tolerance of P. aeruginosa PAO1 and Δdps Strains to H2O2
3.8. Utilization of DNA as a Nutrient Source
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Rajapaksha, N.; Soldano, A.; Yao, H.; Donnarumma, F.; Kashipathy, M.M.; Seibold, S.; Battaile, K.P.; Lovell, S.; Rivera, M. Pseudomonas aeruginosa Dps (PA0962) Functions in H2O2 Mediated Oxidative Stress Defense and Exhibits In Vitro DNA Cleaving Activity. Int. J. Mol. Sci. 2023, 24, 4669. https://doi.org/10.3390/ijms24054669
Rajapaksha N, Soldano A, Yao H, Donnarumma F, Kashipathy MM, Seibold S, Battaile KP, Lovell S, Rivera M. Pseudomonas aeruginosa Dps (PA0962) Functions in H2O2 Mediated Oxidative Stress Defense and Exhibits In Vitro DNA Cleaving Activity. International Journal of Molecular Sciences. 2023; 24(5):4669. https://doi.org/10.3390/ijms24054669
Chicago/Turabian StyleRajapaksha, Nimesha, Anabel Soldano, Huili Yao, Fabrizio Donnarumma, Maithri M. Kashipathy, Steve Seibold, Kevin P. Battaile, Scott Lovell, and Mario Rivera. 2023. "Pseudomonas aeruginosa Dps (PA0962) Functions in H2O2 Mediated Oxidative Stress Defense and Exhibits In Vitro DNA Cleaving Activity" International Journal of Molecular Sciences 24, no. 5: 4669. https://doi.org/10.3390/ijms24054669