SARS-CoV-2 Lysate Stimulation Impairs the Release of Platelet-like Particles and Megakaryopoiesis in the MEG-01 Cell Line
Abstract
:1. Introduction
2. Results
2.1. SARS-CoV-2 Lysate Induces Immature MEG-01 to Release Platelet-like Particles
2.2. SARS-CoV-2 Lysate Interferes with Platelet-like Particle Activation but Not with Cell Viability
2.3. SARS-CoV-2 Lysate Impairs Megakaryopoiesis through STATs Signaling and AMPK Activity
2.4. The Conditioned Medium from SARS-CoV-2-Treated MEG-01 Predisposes Macrophages to Acquire an Anti-Inflammatory Phenotype
3. Discussion
4. Materials and Methods
4.1. SARS-CoV-2 Lysate Production
4.2. Cell Line and Culture Condition
4.3. Platelets Count
4.4. Cytokine Detection
4.5. Flow Cytometry Analysis
4.5.1. Antibodies
4.5.2. Apoptosis Assay
4.5.3. Mitochondrial Activity
4.6. Western Blotting
4.7. Statistical Analysis
Author Contributions
Funding
Conflicts of Interest
References
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Lopardo, V.; Montella, F.; Esposito, R.M.; Zannella, C.; Aliberti, S.M.; Capunzo, M.; Franci, G.; Puca, A.A.; Ciaglia, E. SARS-CoV-2 Lysate Stimulation Impairs the Release of Platelet-like Particles and Megakaryopoiesis in the MEG-01 Cell Line. Int. J. Mol. Sci. 2023, 24, 4723. https://doi.org/10.3390/ijms24054723
Lopardo V, Montella F, Esposito RM, Zannella C, Aliberti SM, Capunzo M, Franci G, Puca AA, Ciaglia E. SARS-CoV-2 Lysate Stimulation Impairs the Release of Platelet-like Particles and Megakaryopoiesis in the MEG-01 Cell Line. International Journal of Molecular Sciences. 2023; 24(5):4723. https://doi.org/10.3390/ijms24054723
Chicago/Turabian StyleLopardo, Valentina, Francesco Montella, Roberta Maria Esposito, Carla Zannella, Silvana Mirella Aliberti, Mario Capunzo, Gianluigi Franci, Annibale Alessandro Puca, and Elena Ciaglia. 2023. "SARS-CoV-2 Lysate Stimulation Impairs the Release of Platelet-like Particles and Megakaryopoiesis in the MEG-01 Cell Line" International Journal of Molecular Sciences 24, no. 5: 4723. https://doi.org/10.3390/ijms24054723