CoNiCrMo Particles, but Not TiAlV Particles, Activate the NLRP3 Inflammasome in Periprosthetic Cells
Abstract
:1. Introduction
2. Results
2.1. Inflammasome Activation in Periprosthetic Tissue of Patients with CoCrMo Alloy Implants Increased Compared to TiAlV Implants
2.2. Characterization of Particles Used for the In Vitro Experiments
2.3. Intracellular Uptake of Particles Did Not Influence the Cell Viability
2.4. Increased ASC-Speck Formation upon Stimulation with CoNiCrMo Particles
2.5. CoNiCrMo Particles Induced Inflammasome-Dependent Signaling in MG63 Cells
3. Discussion
4. Materials and Methods
4.1. Patient Samples
4.2. Immunohistochemistry of ASC
4.3. Cell Culture
4.4. Particle Analysis
4.5. WST-Assay
4.6. Cytoskeleton Staining
4.7. Immunocytochemistry
4.8. Western Blot
4.9. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Brunken, F.; Senft, T.; Herbster, M.; Relja, B.; Bertrand, J.; Lohmann, C.H. CoNiCrMo Particles, but Not TiAlV Particles, Activate the NLRP3 Inflammasome in Periprosthetic Cells. Int. J. Mol. Sci. 2023, 24, 5108. https://doi.org/10.3390/ijms24065108
Brunken F, Senft T, Herbster M, Relja B, Bertrand J, Lohmann CH. CoNiCrMo Particles, but Not TiAlV Particles, Activate the NLRP3 Inflammasome in Periprosthetic Cells. International Journal of Molecular Sciences. 2023; 24(6):5108. https://doi.org/10.3390/ijms24065108
Chicago/Turabian StyleBrunken, Fenna, Tristan Senft, Maria Herbster, Borna Relja, Jessica Bertrand, and Christoph H. Lohmann. 2023. "CoNiCrMo Particles, but Not TiAlV Particles, Activate the NLRP3 Inflammasome in Periprosthetic Cells" International Journal of Molecular Sciences 24, no. 6: 5108. https://doi.org/10.3390/ijms24065108