CCP1, a Regulator of Tubulin Post-Translational Modifications, Potentially Plays an Essential Role in Cerebellar Development
Abstract
:1. Introduction
2. Results
2.1. Ams Mutations and Deletions of CCP1 Alleles Lead to Morphological Changes during the Development of the Mouse Cerebellum
2.2. CCP1 Protein Expression during Cerebellar Development in the Two Mutant Mouse Strains
2.3. Ultrastructural Alterations on PCs Caused by CCP1 Gene Mutation and Knockout
3. Discussion
4. Materials and Methods
4.1. Mice
4.2. Mouse Genotyping
4.3. Tissue Preparation and Morphological Analysis
4.4. Western Blotting
4.5. Transmission Electron Microscope
4.6. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Name | Forward | Reverse |
---|---|---|
Nna1 exon17 | 5′-AATCGGCACAATCCTC-3′ | 5′-TGAACTGACAGATATGTTCATAG-3′ |
Nna1-LOX | 5′-TTACAGACTCCTGCACCTGG-3′ | 5′-GAGTCTGACGCATTACCCAC-3′ |
B2 | 5′-CTTGTACAAAGTGGCCCGAC-3′ |
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Pang, B.; Araki, A.; Zhou, L.; Takebayashi, H.; Harada, T.; Kadota, K. CCP1, a Regulator of Tubulin Post-Translational Modifications, Potentially Plays an Essential Role in Cerebellar Development. Int. J. Mol. Sci. 2023, 24, 5335. https://doi.org/10.3390/ijms24065335
Pang B, Araki A, Zhou L, Takebayashi H, Harada T, Kadota K. CCP1, a Regulator of Tubulin Post-Translational Modifications, Potentially Plays an Essential Role in Cerebellar Development. International Journal of Molecular Sciences. 2023; 24(6):5335. https://doi.org/10.3390/ijms24065335
Chicago/Turabian StylePang, Bo, Asuka Araki, Li Zhou, Hirohide Takebayashi, Takayuki Harada, and Kyuichi Kadota. 2023. "CCP1, a Regulator of Tubulin Post-Translational Modifications, Potentially Plays an Essential Role in Cerebellar Development" International Journal of Molecular Sciences 24, no. 6: 5335. https://doi.org/10.3390/ijms24065335