SiMYBS3, Encoding a Setaria italica Heterosis-Related MYB Transcription Factor, Confers Drought Tolerance in Arabidopsis
Abstract
:1. Introduction
2. Results
2.1. Identification of Drought-Resistant and Heterotic Genes
2.2. Analysis of Drought-Resistant and Heterotic Genes
2.3. Phylogenetic Relationship, Expression Patterns, Subcellular Localization, and Transactivation Activity Analysis of SiMYBS3
2.4. Overexpression of SiMYBS3 in Arabidopsis Led to Improved Drought Tolerance
3. Discussion
3.1. RNA-Seq Can Be Used to Mine Drought Resistance and Heterotic Genes
3.2. Functions of SiMYBS3
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. RNA Extraction, cDNA Synthesis, and RNA-Seq
4.3. WGCNA Analysis and Identification of DEGs
4.4. Cis-Acting Elements and Phylogenetic Tree Analysis
4.5. Real-Time Quantitative PCR
4.6. Subcellular Localization and Transcriptional Activity Analysis
4.7. Phenotypic Observations in Arabidopsis
4.8. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Liu, X.; Zhang, S.; Sun, M.; Guo, Y.; Zhao, S.; Zhou, X.; Bai, X.; Dai, K.; Li, H.; Yuan, X.; et al. SiMYBS3, Encoding a Setaria italica Heterosis-Related MYB Transcription Factor, Confers Drought Tolerance in Arabidopsis. Int. J. Mol. Sci. 2023, 24, 5418. https://doi.org/10.3390/ijms24065418
Liu X, Zhang S, Sun M, Guo Y, Zhao S, Zhou X, Bai X, Dai K, Li H, Yuan X, et al. SiMYBS3, Encoding a Setaria italica Heterosis-Related MYB Transcription Factor, Confers Drought Tolerance in Arabidopsis. International Journal of Molecular Sciences. 2023; 24(6):5418. https://doi.org/10.3390/ijms24065418
Chicago/Turabian StyleLiu, Xin, Shuai Zhang, Mengbo Sun, Yurong Guo, Shaoxing Zhao, Xutao Zhou, Xionghui Bai, Keli Dai, Huixia Li, Xiangyang Yuan, and et al. 2023. "SiMYBS3, Encoding a Setaria italica Heterosis-Related MYB Transcription Factor, Confers Drought Tolerance in Arabidopsis" International Journal of Molecular Sciences 24, no. 6: 5418. https://doi.org/10.3390/ijms24065418