Transcriptomic Analysis Reveals the Detoxification Mechanism of Chilo suppressalis in Response to the Novel Pesticide Cyproflanilide
Abstract
:1. Introduction
2. Results
2.1. Bioassay Results
2.2. Field Experiment Results
2.3. Transcriptome Analysis
2.3.1. Sample Quality Control and Correlation Analysis of 6 Samples
2.3.2. GO and KEGG Analysis of DEGs
2.3.3. Transcriptome Profiling Reveals Detoxification Genes Associated with Cyproflanilide
2.4. Analysis Detoxification Gene Expression Level by qRT-PCR
2.5. Sensitivity to Pesticides after Gene Silencing
3. Discussion
4. Materials and Methods
4.1. Insect and Insecticide
4.2. Bioassay
4.3. Evaluting and Verifying Field Efficacy
4.4. RNA Sequencing and Annotation of Unigenes
4.5. Quantitative Real Time PCR
4.6. RNAi Experiment
4.7. Data Processing and Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Insecticide | Degree of Freedom | χ2 | LD10 Value (95% Confidence Interval) | LD30 Value (95% Confidence Interval) | LD50 value (95% Confidence Interval) |
---|---|---|---|---|---|
Cyproflanilide solution | 5 | 5.42 | 1.7 ng/per larva (1.034–1.8306) | 5.2612 ng/per larva (4.3203–6.4241) | 13.02 ng/per larva (10.3713–17.1871) |
Treatment Groups | Active Ingredient (g/hectare) | Control Effect after 21 Days (%) | Significance of Difference (5%) |
---|---|---|---|
20% Cyproflanilide SC | 16.2 | 77.38 ± 2.54 | b |
32.4 | 84.6 ± 5.09 | ab | |
48.6 | 87.26 ± 3.58 | a | |
64.8 | 91.24 ± 2.72 | a | |
200 g/L Chlorantraniliprole® | 30.0 | 35.17 ± 1.36 | c |
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Zhou, J.; Qiu, L.; Liang, Q.; Zhou, Y.; Sun, J.; Gao, Q.; He, H.; Ding, W.; Xue, J.; Li, Y. Transcriptomic Analysis Reveals the Detoxification Mechanism of Chilo suppressalis in Response to the Novel Pesticide Cyproflanilide. Int. J. Mol. Sci. 2023, 24, 5461. https://doi.org/10.3390/ijms24065461
Zhou J, Qiu L, Liang Q, Zhou Y, Sun J, Gao Q, He H, Ding W, Xue J, Li Y. Transcriptomic Analysis Reveals the Detoxification Mechanism of Chilo suppressalis in Response to the Novel Pesticide Cyproflanilide. International Journal of Molecular Sciences. 2023; 24(6):5461. https://doi.org/10.3390/ijms24065461
Chicago/Turabian StyleZhou, Jungang, Lin Qiu, Qiyao Liang, Yi Zhou, Jinjun Sun, Qiao Gao, Hualiang He, Wenbing Ding, Jin Xue, and Youzhi Li. 2023. "Transcriptomic Analysis Reveals the Detoxification Mechanism of Chilo suppressalis in Response to the Novel Pesticide Cyproflanilide" International Journal of Molecular Sciences 24, no. 6: 5461. https://doi.org/10.3390/ijms24065461